Ultratrace DNA Detection Based on the Condensing‐Enrichment Effect of Superwettable Microchips

Xu, Li-Ping; Chen, Yanxia; Gao, Yang; Shi, Wanxin; Dai, Bing; Li, Guannan; Cao, Yanhua; Wen, Yongqiang; Zhang, Xueji; Wang, Shutao

doi:10.1002/adma.201502982

Cited by 135 publications

(97 citation statements)

References 37 publications

(7 reference statements)

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“…Until now, SPW had been extensively explored as an effective high‐throughput platform for bioassay and biosensing . For example, Wang group developed SPW for various biosensing, such as sensitive nucleic acid detection based on condensing‐enrichment effect, microgravity biosensing and electrochemical detection toward cancer biomarkers . Noticeably, the specific micro‐/nanostructure of the (super)wetting domains can be helpful for the designed detections.…”

Section: Applicationsmentioning

confidence: 99%

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Duan

Zheng

Zhao

et al. 2019

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Understanding the relationship between liquid manipulation and micro‐/nanostructured interfaces has gained much attention due to the wide potential applications in many fields, such as chemical and biomedical assays, environmental protection, industry, and even daily life. Much work has been done to construct various materials with interfacial liquid manipulation abilities, leading to a range of interesting applications. Herein, different fabrication methods from the top‐down approach to the bottom‐up approach and subsequent surface modifications of micro‐/nanostructured interfaces are first introduced. Then, interactions between the surface and liquid, including liquid wetting, liquid transportation, and a number of corresponding models, together with the definition of hydrophilic/hydrophobic, oleophilic/olephobic, the definition and mechanism of superwetting, including superhydrophobicity, superhydrophilicity, and superoleophobicity, are presented. The micro‐/nanostructured interface, with major applications in self‐cleaning, antifogging, anti‐icing, anticorrosion, drag‐reduction, oil–water separation, water collection, droplet (micro)array, and surface‐directed liquid transport, is summarized, and the mechanisms underlying each application are discussed. Finally, the remaining challenges and future perspectives in this area are included.

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Section: Applicationsmentioning

confidence: 99%

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Duan

Zheng

Zhao

et al. 2019

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“…Rough 3D nanostructured surfaces have garnered much interest for microfluidics and biosensor development . For the same apparent surface area, the 3D nanostructured surfaces provide more binding sites for capture probes and targets, generate different surface properties (e.g., superhydrophobicity) from the flat surface, enhance detection signals, and supply topological and morphological features to encourage the interaction between device surface and nanoscale objects, such as proteins, nucleic acid molecules, and the microfilament/pseudopodium of cells . Meanwhile, the diffusion distance is efficiently reduced for antibodies and pathogens within the nanostructures, which accelerates the diffusion‐dependent steps in pathogen capture and detection.…”

Section: Introductionmentioning

confidence: 99%

A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection

Xia

Tang

et al. 2017

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Rapid and simultaneous detection of multiple potential pathogens by portable devices can facilitate early diagnosis of infectious diseases, and allow for rapid and effective implementation of disease prevention and treatment measures. The development of a ZnO nanorod integrated microdevice as a multiplex immunofluorescence platform for highly sensitive and selective detection of avian influenza virus (AIV) is described. The 3D morphology and unique optical property of the ZnO nanorods boost the detection limit of the H5N2 AIV to as low as 3.6 × 10 EID mL (EID : 50% embryo infectious dose), which is ≈22 times more sensitive than conventional enzyme-linked immunosorbent assay. The entire virus capture and detection process could be completed within 1.5 h with excellent selectivity. Moreover, this microfluidic biosensor is capable of detecting multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 AIV can be released by simply dissolving ZnO nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

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“…Sensing microchips show great application promises in modern chemistry, biology, and medical science. Especially, sensing microchips are suitable for on‐the‐site analysis such as screening of infectious diseases, point‐of‐care testing of biomarkers, and rapid detection of chemical and biological warfare agents …”

Section: Introductionmentioning

confidence: 99%

“…This hydrophilic–hydrophobic pattern (wettability pattern) not only retains the ability of superhydrophobic surface to prevent liquid spread and limit the size of dried solute, but also avoids the problem of poor reproducibility caused by drop shift. Wang and co‐workers designed a superwettable pattern for ultratrace DNA detection . In their work, superhydrophilic microwells were fabricated on a superhydrophobic hierarchical silica coating, which was then adapted as a substrate for fluorescence‐based DNA detection.…”

Section: Introductionmentioning

confidence: 99%

Paper‐Based Hydrophobic/Lipophobic Surface for Sensing Applications Involving Aggressive Liquids

Zhang

Ren

et al. 2016

Adv Materials Inter

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Biological and chemical sensing microchips based on surface wettability (or wettability difference) attract much interest due to associated advantages such as high sensitivity, ease of use, and time saving. Wettability is the wetting behavior of liquid on solid surface, which is usually evaluated by liquid contact angles on solid surface. Superhydrophobic (extreme nonwetting) behavior is generally defined by high apparent contact angle (>150°) and low sliding angle (<10°). [3] It is found that water solution dropped on superhydrophobic surface forms round bead shape. As water vapors, the solution would be condensed allowing the solute enrichment on the contact area between water drop and solid surface. Ling group fabricated a superhydrophobic SERS (surfaceenhanced Raman scattering) platform by assembling Ag nanocubes that support strong surface plasmon for trace detection. [4] This design integrates the intense electromagnetic field confinement generated by Ag nanocubes with a superhydrophobic surface capable of analyte concentration. This strategy demonstrates a detection limit down to 10 −16 m with sample volume of just 1 μL. Alam group developed a fast, non-faradaic impedance sensing method for synthetic DNA by employing adhesive hydrophobic surface to allow robust pinning of analyte droplet to the sensor surface and concentrate DNA target on the contact zone. [5] The detection limit of this method is improved by five orders of magnitude.Basically, due to the low adhesion (low sliding angle) of superhydrophobic surface, round-bead shaped water drops easily roll off or shift on superhydrophobic surface. [3] Therefore, superhydrophobic surface is generally not beneficial for accurate analysis and exquisite reactions requiring multiple operations. In order to conquer this problem, researchers fabricated hydrophilic micropatterns on superhydrophobic surfaces to pin liquid drops. This hydrophilic-hydrophobic pattern (wettability pattern) not only retains the ability of superhydrophobic surface to prevent liquid spread and limit the size of dried solute, but also avoids the problem of poor reproducibility caused by drop shift. Wang and co-workers designed a superwettable pattern for ultratrace DNA detection. [2] In their work, superhydrophilic microwells were fabricated on a superhydrophobic hierarchical silica coating, which was then adapted as a substrate for fluorescence-based DNA detection. Benefitting from the superwettability difference between the microwell and the surrounding substrate, the analytes were enriched from A paper-based hydrophobic/lipophobic surface with high liquid drop adhesion capable of carrying out sensitive biochemical assays directly is reported. Hydrophobic sol-gel derived fluoroalkyl-silica is used to develop the hydrophobic/lipophobic surface, which demonstrates superior confining abilities. The reported hydrophobic/lipophobic surface could retain aggressive liquids such as organic solvents and surfactant solutions whereas the methyl-silica based surface fails to function. Because of t...

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Ultratrace DNA Detection Based on the Condensing‐Enrichment Effect of Superwettable Microchips

Cited by 135 publications

References 37 publications

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection

Paper‐Based Hydrophobic/Lipophobic Surface for Sensing Applications Involving Aggressive Liquids

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