Ultrathin Phthalocyanine-Conjugated Polymer Nanosheet-Based Electrochemical Platform for Accurately Detecting H₂O₂ in Real Time

Abstract

As a vital biological mediator and a widely used industrial oxidant, the accurate detection of hydrogen peroxide (H 2 O 2 ) is of significance for both academic purpose and practical applications. Herein, we report a novel approach for the development of a high-performance electrochemical H 2 O 2 sensor constructed by iron phthalocyanine (FePc)based diyne-linked conjugated polymeric nanosheets (NSs), FePc-CP NSs. The FePc-CP NSs were delaminated from the bulk material via a defect-and disorder-induced synthetic strategy. By the quasi-Langmuir−Shafer method, the prepared FePc-CP NSs were self-assembled into multilayer films with controllable thickness on electrodes. Owing to the highly exposed active centers on the surfaces, the FePc-CP NS filmmodified electrodes exhibited excellent H 2 O 2 determination performance with a wide linear detection range (0.1−1000 μM), a short response time (the response current approached the maximum value within 0.1 s), a low limit of detection (0.017 μM), and excellent sensitivity (97 μA cm −2 mM −1 ), which are comparable to the best results reported so far for electrochemical H 2 O 2 sensors. In addition, the fabricated electrochemical H 2 O 2 sensor also displayed satisfactory stability, reproducibility, and selectivity. Furthermore, the obtained FePc-CP NS film sensor can be applied in real-time monitoring of H 2 O 2 in commercial orange juice and beer as well as H 2 O 2 secreted from A549 live cells, revealing its application potential toward the accurate detection of H 2 O 2 in real-sample analysis.

Mixed Co‐Mn Spinel Oxides Based Electrocatalysts for Amperometric Determination of Hydrogen Peroxide

“…Hydrogen peroxide (H 2 O 2 ), an indispensable chemical, plays significant roles in various industries, such as, textile, papermaking, food manufacturing, and pharmaceuticals [1–3] . However, in the food industries, the overuse of H 2 O 2 is detrimental for the health of human‐beings due to its toxicity according to GB 2760‐86 (hygienic standard of food additives of China).…”

“…Hydrogen peroxide (H 2 O 2 ), an indispensable chemical, plays significant roles in various industries, such as, textile, papermaking, food manufacturing, and pharmaceuticals. [1][2][3] However, in the food industries, the overuse of H 2 O 2 is detrimental for the health of human-beings due to its toxicity according to GB 2760-86 (hygienic standard of food additives of China). In addition, H 2 O 2 has been identified to be involved in many physiological processes, where the high-level secretion of H 2 O 2 from living organisms always associates with some severe illness, like diabetes, cancer, atherosclerosis, Parkinson's disease.…”

Non‐covalent Immobilization of Iron‐triazole (Fe(Htrz)3) Molecular Mediator in Mesoporous Silica Films for the Electrochemical Detection of Hydrogen Peroxide

“…They include protein‐based electrochemical biosensors and nonenzymatic sensors involving the use of either charge transfer mediators or nanomaterials , or a combination of both , or even nanocomposites (such as Pt nanoparticles and polyaniline hosted in mesoporous silica film ), in order to lower the high overpotentials usually associated to the electrochemical transformation of H 2 O 2 . Widely used mediators for H 2 O 2 sensing are Prussian Blue and related derivatives as well as organic or organometallic compounds , often deposited onto electrode surfaces as polymeric coatings or as covalently attached moieties . Free molecular organic redox mediators are much less used because the resulting devices may suffer from poor long‐term stability.…”

Graphitic Carbon Nitride/Poly(3-hexylthiophene) Nanocomposites for the Photoelectrochemical Detection of H2O2 in Living Cells

“…Hydrogen peroxide (H 2 O 2 ), one of the most stable active O species, is involved in many important vital movements in organisms, such as cell proliferation, differentiation, senescence, and carcinogenesis. , Abnormalities in H 2 O 2 levels are detrimental to proteins, lipids, and nucleic acids, bring strong cytotoxicity, and thus are associated with many diseases, such as Parkinson’s, Alzheimer’s, cancer, and cardiovascular diseases . Therefore, the sensitive and selective detection of H 2 O 2 , especially secreted from living cells as an important biomarker for these diseases, is essential for early diagnosis.…”

“…1,2 Abnormalities in H 2 O 2 levels are detrimental to proteins, lipids, and nucleic acids, bring strong cytotoxicity, and thus are associated with many diseases, such as Parkinson's, Alzheimer's, cancer, and cardiovascular diseases. 3 Therefore, the sensitive and selective detection of H 2 O 2 , especially secreted from living cells as an important biomarker for these diseases, is essential for early diagnosis. Many methods have been proposed to detect H 2 O 2 , such as fluorescence, colorimetry, chemiluminescence, electrochemistry, and photoelectrochemistry (PEC) methods.…”