Ultrathin films of a metal-organic framework prepared from 2-methylimidazole, manganese(II) and cobalt(II) with strong oxidase-mimicking activity for colorimetric determination of glutathione and glutathione reductase activity

Zhang, Yi; Dai, Changlian; Liu, Wei; Wang, Yanying; Ding, Fang; Zou, Ping; Wang, Xianxiang; Zhao, Qingbiao; Rao, Hanbing

doi:10.1007/s00604-019-3434-5

Cited by 30 publications

(7 citation statements)

References 33 publications

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“…Table 1 showed the analytical results of GSH concentration and GR activity in the spiked serum samples. According to the obtained calibration curves, the concentrations of GSH and GR in human serum sample were calculated to be 0.34 mM and 25.5 mU/mL, respectively, and the results were consistent with the previous reports [ 27 , 28 ]. Moreover, the recoveries were measured between 98.77% and 108.86% with the relative standard deviation (RSD) values lower than 9.27%, indicating that the colorimetric sensing system showed good anti-interference capacity for the detection of GSH and GR activity in human serum samples.…”

Section: Resultssupporting

confidence: 91%

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Lai

Liao

et al. 2021

Molecules

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Abnormal levels of reduced glutathione (GSH) and glutathione reductase (GR) are usually related to a variety of diseases, so it is of great significance to determine the GSH concentration and GR activity. We herein develop a smartphone-assisted colorimetric biosensor for the detection of GSH and GR activity in human serum and mouse liver using hemin/G-quadruplex DNAzyme. Firstly, an obvious color change from colorless to green can be observed, owing to the high peroxidase-like activity of hemin/G-quadruplex DNAzyme toward 2,2′-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS). With the addition of GSH or GR, the H2O2-mediated oxidation of ABTS catalyzed by hemin/G-quadruplex DNAzyme is significantly inhibited, resulting in remarkable color fading. Therefore, the detection of GSH and GR activity can be achieved by observing the color transition or measuring the absorbance at 420 nm. The detection limit was estimated to be as low as 0.1 μM and 10 μU/mL for GSH and GR, respectively. More interestingly, the RGB values of the sensing system can be identified by the smartphone application (APP, color collect), which makes it an ideal format for on-site determination and point-of-care testing (POCT). In addition, the proposed method shows excellent selectivity and acceptable applicability for the determination of GSH concentration and GR activity in human serum samples and mouse liver tissues, which might hold great application potential in clinical diagnosis and drug screening.

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Section: Resultssupporting

confidence: 91%

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Lai

Liao

et al. 2021

Molecules

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“…A negatively charged surface is favorable for a-BiVO 4 interacting with TMB, which increases the catalytic efficiency because it facilitates charge transfer . It has been reported that nanozymes, including Co 2 V 2 O 7, Mn–Co NS, Co 3 O 4 /CuO, and NiCo 2 S 4 –rGO, need a suitable temperature to maintain their activities. As shown in Figure S7, a-BiVO 4 still has high oxidase-like activity at 5 °C.…”

Section: Resultsmentioning

confidence: 99%

Oxygen Vacancy-Rich Amorphous BiVO₄ Nanoparticles for Colorimetric Sensing

Chen

et al. 2023

ACS Appl. Nano Mater.

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Defects in crystalline nanozymes play a critical role in enhancing their performances and related catalytic events. However, creating a high concentration of active and robust defects in well-explored crystalline metal oxides to achieve high-performance single-enzymatic activity is challenging. Herein, amorphous BiVO4 beyond traditional crystalline nanozymes is prepared via a facile solution-processed coprecipitation method favorable to large-scale manufacturing to mimic enzymatic properties. As expected, the specially designed amorphous material without any post-treatments has sufficient and robust oxygen vacancies, which are highly active in converting oxygen to reactive radicals (O2 –•). Simultaneously, the negatively charged surface has a high affinity for the substrate, facilitating charge transfer between materials and substrates and, in turn, providing higher catalytic efficiency compared to the crystalline counterparts. Most importantly, the sample can only mimic oxidase-like activity rather than the multienzyme-like activity generally observed in most reported crystalline metal oxides, which is a step forward in the development of nanozymes. This amorphous BiVO4-based system is also evaluated for the sensitive and selective detection of l-cysteine. The study provides insight into nanozyme development and highlights the great potential of amorphous materials in addition to amorphous BiVO4.

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“…Fe–N–C acts as the catalyst, organic substrates act as electron donors, molecular oxygen or H 2 O 2 acts as the electron acceptor and is reduced according to the nature of enzyme catalysis. 30 In this system, Fe( ii ) on the surface of Fe–N–C plays a leading role in reacting with H 2 O 2 to produce ˙OH. The process can be described in the following reactions: 31 Fe III + H 2 O 2 → Fe II + ˙OOH + H + Fe III + ˙OOH → Fe II + O 2 + H + Fe II + H 2 O 2 → Fe III + ˙OH + OH − ˙OH + TMB → ox-TMB + H + 2 O…”

Section: Resultsmentioning

confidence: 99%

Magnetic Fe–N–C nanoparticles as a dual nanozyme for label-free colorimetric detection of antibiotics

Wen

Liu

et al. 2023

Environ. Sci.: Adv.

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Ultrathin films of a metal-organic framework prepared from 2-methylimidazole, manganese(II) and cobalt(II) with strong oxidase-mimicking activity for colorimetric determination of glutathione and glutathione reductase activity

Cited by 30 publications

References 33 publications

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Oxygen Vacancy-Rich Amorphous BiVO₄ Nanoparticles for Colorimetric Sensing

Magnetic Fe–N–C nanoparticles as a dual nanozyme for label-free colorimetric detection of antibiotics

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Ultrathin films of a metal-organic framework prepared from 2-methylimidazole, manganese(II) and cobalt(II) with strong oxidase-mimicking activity for colorimetric determination of glutathione and glutathione reductase activity

Cited by 30 publications

References 33 publications

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Smartphone-Assisted Colorimetric Detection of Glutathione and Glutathione Reductase Activity in Human Serum and Mouse Liver Using Hemin/G-Quadruplex DNAzyme

Oxygen Vacancy-Rich Amorphous BiVO4 Nanoparticles for Colorimetric Sensing

Magnetic Fe–N–C nanoparticles as a dual nanozyme for label-free colorimetric detection of antibiotics

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Product

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Oxygen Vacancy-Rich Amorphous BiVO₄ Nanoparticles for Colorimetric Sensing