Ultrastrukturelle Aspekte des supraoptikohypophysären Systems in der hypophysektomierten Ratte

An immunohistological and electron microscopic investigation of neurosecretory neuronal regeneration following hypophysial stalk transection in animals subjected to chronic intermittent salt loading

“…Retrograde degeneration occurs in the magnocellular cells in the SON and PVN after axotomy of the HNT [47,124,125,173,174,195]. During the so-called obstructive phase, beginning 1 or 2 days after hypophysectomy and lasting for 10 days [195], accumulated neurosecretory material spreads back firom the proximal transection site to the cell bodies [195].…”

“…The nuclei and cytoplasm become progressively more dense. The cytoplasm contains autophagic vacuoles and dense bodies [173,174]. Between 6 and 9 postoperative days, the degenerating cells become even more shrunk and spherical, and contain more dense lamellar bodies.…”

“…Ultrastructurally, the reaction changes in axotomized neurons which are not degenerating are first recognized one day after axotomy, appearing as a few NGVs and dense bodies in the cell bodies [124,125]. After three days, they become more conspicuous and are characterized by an extensive rER which is either fragmented or in a peripheral location [123,124,173,174,195]. These cells also contain many ribosomes and NGVs which are most abimdantly accumulated in the periphery and are intermingled with dense bodies and multivesicular bodies [173,174].…”

“…After three days, they become more conspicuous and are characterized by an extensive rER which is either fragmented or in a peripheral location [123,124,173,174,195]. These cells also contain many ribosomes and NGVs which are most abimdantly accumulated in the periphery and are intermingled with dense bodies and multivesicular bodies [173,174]. Between 9 to 12 postoperative days, the surviving cells are enlarged and contain more NGVs, dense bodies and multivesicular bodies [47].…”

“…These enlarged neurons have extensive rER and Golgi apparatus [195]. The NGVs are no longer accimiulated in the periphery and dense bodies are smaller and less nimierous [173,174].…”