Ultrastrukturanalyse oberflächenmodifizierter Intraokularlinsen mit Hilfe der Rasterkraftmikroskopie

Ettl, Armin; Pum, Dietmar; Schmid, E. D.; Daxer, Albert; Göttinger, W.

doi:10.1007/978-3-642-50185-2_60

Cited by 2 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…scanning electron microscopy, which allows visualization of aggregates of short-chained heparin molecules as globular structures. Ettl et al [24] using high magnifications in HSM IOLs found small areas (diameter: approximately 50-150 nm) not showing the usual globular structure. Mechanical artifacts explaining these defects in scanning electron microscopy could not be ruled out.…”

Section: Discussionmentioning

confidence: 99%

A New Method for Direct Detection of Heparin on Surface-Modified Intraocular Lenses

Dick¹,

Eisenmann³

et al. 1997

Ophthalmologica

View full text Add to dashboard Cite

Background: Examination of surface-bound heparin on synthetic polymers can only be performed by few staining methods. These methods are limited by an only approximate detection of heparin visible at high magnification. Other methods only measure heparin quantitatively (per square dimension) and are rather sensitive to artifacts. Due to its homogeneous staining pattern, the modified toluidine blue staining technique, using a non-protein-based substance, allows examination and analysis of the homogeneity of the monomolecular heparin layer even under critical conditions like scanning electron microscopy. Materials and Methods: For critical examination of the heparin layer, this method was used for 5 sterile heparin surface-modified (HSM) monofocal (Pharmacia, 809C, 5 mm optical zone), 2 multifocal PMMA (Pharmacia, 81 IE, 6 mm optical zone) and 1 standard PMMA intraocular lenses (IOLs; Pharmacia, 809P, 5 mm optical zone). A special mixture containing a sodium borate buffer was used to avoid cross-reactions of toluidine with phenylimines, which permit a covalent surface linkage of heparin to synthetic polymeric materials via reductive amination. This resulted in less coarse-grained complex agglutination. After light and spectral microscopy of the surface of stained IOLs, scanning electron microscopy was performed to investigate the reliability and validity of this modified staining method. Results: All HSM IOLs showed a homogeneous heparin structure and coating, which could be demonstrated even under critical photographic circumstances. Conclusions: The modification of the original toluidine blue staining method introduced by Jaques in 1943 is a reliable and reproducible technique for the detailed in vitro analysis of surface-bound heparin with a low artifact rate. Because of the detailed detection of heparin on polymeric surfaces, this staining method is recommended for special problems, e.g. in cataract surgery.

show abstract

Section: Discussionmentioning

confidence: 99%