Ultrastructure of Tuta absoluta parasitized eggs and the reproductive potential of females after parasitism by Metarhizium anisopliae

Pires, Laurici Maria; Marques, Edmilson Jacinto; Wanderley‐Teixeira, Valéria; Teixeira, Álvaro Aguiar Coelho; Alves, Luiz Cláudio Pinto de Sá; Alves, Elza

doi:10.1016/j.micron.2008.07.008

Cited by 27 publications

(23 citation statements)

References 17 publications

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“…There are no registers of the effects of entomopathogens on P. viburni, however, it is possible to make comparisons with other insects. Similar results to this study were observed in the reproductive output of Tutta absoluta (Pires et al, 2008), using doses of 10 7 conidia mL -1 of M. anisopliae and Diuraphis noxia (Wang and Knudsen, 1993) using B. bassiana. Other studies have shown positive results on this topic, for example Cylas puncticollis (Ondiaka et al, 2008), Anoplophora glabripennis (Hajek et al, 2008), Megalurothrips sjostedti (Ekesi and Maniania, 2000), among others.…”

Section: Virulencesupporting

confidence: 90%

“…This could be due to sample heterogeneity, evidenced by the high coefficient of variation (CV= 77%) not being possible to detect statistical differences. The distribution of the number of eggs laid throughout the female life span does not change among treatments when exposed to M. anisopliae (Figure 2), which differs to other studies where insects exposed to entomopathogenic fungi have shown higher initial number of eggs than the control, however, without statistical differences (Arthurs and Thomas, 2000;Pires et al, 2008). The development of a disease produced by a pathogen could be affected by biotic factors on the host, such as reproductive properties characteristics of the insect, population characteristics (individual susceptibility), and insect habits, among others (Alves and Lecuona, 1998).…”

Section: Virulencecontrasting

confidence: 79%

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Virulence and Pre-Lethal Reproductive Effects of Metarhizium anisopliae var. anisopliae on Pseudococcus viburni (Hemiptera: Pseudococcidae)

Pereira¹,

Casals²,

Salazar

et al. 2011

Chilean J. Agric. Res.

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Obscure mealybug, Pseudococcus viburni (Signoret), is a cosmopolitan pest that causes damage by suction of vascular juices and the production of honeydew, as well as for being a quarantine insect. Within control options, entomopathogenic fungi are a good alternative, nevertheless, more research is needed. In this research, the Metarhizium anisopliae var. anisopliae (Metschnikoff) isolate Qu-M984 was evaluated on P. viburni under laboratory conditions. Virulence was evaluated by lethal dose 50 (LD 50 ) and lethal time 50 (LT 50 ), for each of the four life stages of the female. The doses tested were 10 5 , 10 6 , 10 7 and 10 8 conidia mL -1 . Also fecundity, egg size, fertility, and longevity of adult females were evaluated at doses of 10 5 and 10 6 conidia mL -1 , the evaluations were made every 2 d throughout the insect life time. The LD 50 and LT 50 obtained were variable for each life stage, although without statistical differences among life stages (P > 0.05), ranging from LD 50 = 7.3 × 10 5 to 4.9 × 10 9 conidia mL -1 and LT 50 = 7.74 to 9.97 d at 10 8 conidia mL -1 . Significant differences (P ≤ 0.05) were observed on longevity at 10 6 conidia mL -1 . Longevity was 29% less compared to the control. This result on longevity at relatively low dose is relevant due to the fact that decrease possibilities to find live quarantine insects at the moment of harvest. Fertility, fecundity and egg size showed no differences (P > 0.05).

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Section: Virulencesupporting

confidence: 90%

Section: Virulencecontrasting

confidence: 79%

Virulence and Pre-Lethal Reproductive Effects of Metarhizium anisopliae var. anisopliae on Pseudococcus viburni (Hemiptera: Pseudococcidae)

Pereira¹,

Casals²,

Salazar

et al. 2011

Chilean J. Agric. Res.

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“…In all cases, the cumulative mortality remained stable from the 15th day after inoculation, which implied that the parasitic relationship of these strains with C. nucum larvae was unstable. In some previous studies, M. anisopliae has been found to attach to and germinate on the host cuticle within a few hours after inoculation [21,22]. However, our SEM results indicated that M. anisopliae var.…”

Section: Discussioncontrasting

confidence: 77%

Evaluation of Pathogenicity of the Fungi Metarhizium anisopliae and Beauveria bassiana in Hazelnut Weevil (Curculio nucum L., Coleoptera, Curculionidae) Larvae

et al. 2016

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The nut weevil (Curculio nucum) is one of the most important and widespread pests in hazelnut orchards. In order to screen entomopathogenic fungal strains with high virulence against C. nucum, the growth rate, sporulation, and cumulative mortality of different Metarhizium anisopliae and Beauveria bassiana strains were investigated, and the process by which M. anisopliae CoM 02 infects C. nucum larvae was observed using scanning electron microscopy. The results indicated that the growth rate and sporulation of different fungal strains significantly differed. Thirteen days after inoculation with M. anisopliae CoM 02, the cumulative mortality of C. nucum larvae reached 100 %, which was considerably higher than that of the other five strains. As the most virulent of the six test strains, the cadaver rate, LT 50 , and LT 90 of M. anisopliae CoM 02 were 93.4 %, 7.05 and 11.90 days, respectively. Analysis of the infection process by scanning electron microscopy showed that the spore attachment, hyphal germination, hyphal rapid growth, and sporulation of M. anisopliae CoM 02 occurred on the 3rd, 5th, 7th, and 11th day after inoculation, respectively, indicating that the infection cycle takes approximately 11 days. This finding suggests that the highly virulent M. anisopliae plays an important role in the biocontrol of C. nucum in China.

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“…Vestergaard et al (1999) observed M. anisopliae conidia frequently on the wings and legs of Frankliniella occidentalis. Conidia attachment, however, has been found in different regions on Tuta absoluta (Pires et al 2009) or on Heterotermes tenuis (Moino et al 2002) by M. anisopliae. These results indicate a great variation in the density and germination of the conidia in different parts of the insect body, which are primarily found next to the natural openings or in soft tissues (Kirkland et al 2004).…”

Section: Discussionmentioning

confidence: 98%

Virulence of the entomopathogenic fungus Metarhizium anisopliae using soybean oil formulation for control of the cotton stainer bug, Dysdercus peruvianus

Santi

Silva

Beys‐da‐Silva

et al. 2011

World J Microbiol Biotechnol

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The cotton stainer bug Dysdercus peruvianus (Hemiptera: Pyrrhocoridae) is an insect pest that causes heavy losses in cotton plantations. The need to reduce the use of insecticides for control of this pest has increased steadily, and Metarhizium anisopliae (Ascomycota: Clavicipitaceae) could be an important biopesticide candidate to control this pest. The effect of M. anisopliae on D. peruvianus nymphs and adults using formulations with soybean oil and Agral Ò was evaluated. Formulation using 10% soybean oil added to 10 8 conidia mL -1 (grown on used and reused rice) was the most effective for nymph and adult, causing 100% mortality 6 and 7 days after exposure, respectively. The SEM analysis of infected insects showed that M. anisopliae conidia were able to adhere anywhere on the exoskeleton, but were more abundant between the joints. Using the same rice for two batches of growth may be an option for improving commercial conidial production of M. anisopliae and may reduce overall costs. Its effect on D. peruvianus adults opens a new possibility for using this fungus as an alternative to chemical pesticides and the use of M. anisopliae in association with integrate pest management.

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Ultrastructure of Tuta absoluta parasitized eggs and the reproductive potential of females after parasitism by Metarhizium anisopliae

Cited by 27 publications

References 17 publications

Virulence and Pre-Lethal Reproductive Effects of Metarhizium anisopliae var. anisopliae on Pseudococcus viburni (Hemiptera: Pseudococcidae)

Virulence and Pre-Lethal Reproductive Effects of Metarhizium anisopliae var. anisopliae on Pseudococcus viburni (Hemiptera: Pseudococcidae)

Evaluation of Pathogenicity of the Fungi Metarhizium anisopliae and Beauveria bassiana in Hazelnut Weevil (Curculio nucum L., Coleoptera, Curculionidae) Larvae

Virulence of the entomopathogenic fungus Metarhizium anisopliae using soybean oil formulation for control of the cotton stainer bug, Dysdercus peruvianus

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