Ultrastructure of the myotendinous junction of the medial pterygoid muscle of adult and aged Wistar rats

Ciena, Adriano Polican; Luques, Isabela Ugo; Días, Fernando José; Almeida, Sonia Regina Yokomizo de; Iyomasa, Mamie Mizusaki; Watanabe, Ii-sei

doi:10.1016/j.micron.2010.04.006

Cited by 29 publications

(24 citation statements)

References 24 publications

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“…In addition, it acts directly on joint stabilization and strength (Kannus, Jozsa, Kvist, Lehto, & Järvinen, 1992). Ciena et al (2010) revealed similar morphological adaptations when they described the MTJ morphology of the medial pterygoid of elderly rats. Meng and Yu (2010) state that one of the several causes of sarcopenia may be characterized by hormonal changes, inflammation, and physical inactivity.…”

Section: Morphometrymentioning

confidence: 74%

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Sierra

Fávaro

Cerri

et al. 2018

Microscopy Res & Technique

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The study aims to describe the tissue plasticity of MTJ through the morphological analysis of MTJ soleus in ovariectomized aged female Wistar rats submitted to aquatic training. Forty aged Wistar rats, 1 year and 2 months of age, were divided into four groups: sedentary (S), trained (T), ovariectomized (O), and trained/ovariectomized (OT). Employing the transmission electron microscopy, the ultrastructural and morphometric elements were revealed. In the S group, changes in morphological characteristics as a consequence of the aging process were seen, demonstrated by the conical shape of the muscle cell extremity, a large area with collagen deposit, and misalignment of sarcomeres in series. The T group presented ample adjustments when revealed the organization of MTJ, through the increase of the contact area and greater lengths of sarcoplasmatic invaginations and evaginations. The O group revealed extensive tissue disorganization with muscle atrophy, reduction of MTJ contact area, and consequently, changes in sarcoplasmatic invaginations and evaginations. The OT group demonstrated extensive remodeling with restructuring MTJ through the increase of tissue contact area, extensive organization, parallel arrangement, and increased length of sarcoplasmatic invaginations and evaginations. The distal sarcomeres presented higher lengths compared to the proximal sarcomeres in both the groups. We conclude that aquatic training was effective in the organization and structural remodeling of the myotendinous interface of ovariectomized aged rats. There was a greater area of contact, and consequently, greater resistance in the myotendinous interface promoting a lower predisposition to injuries.

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Section: Morphometrymentioning

confidence: 74%

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Sierra

Fávaro

Cerri

et al. 2018

Microscopy Res & Technique

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“…For light microscopy analysis, animals were anesthetized intraperitoneally with urethane overdose (3 g/kg, i.p.) (Ciena et al,2010). TMJs were removed in a block containing the skull base, the mandible, and adjacent soft tissues and fixed in Bouin's solution.…”

Section: Methodsmentioning

confidence: 98%

Biophysical characterization of polymyxin b interaction with LPS aggregates and membrane model systems

Domingues¹,

Inácio²,

Raimundo³

et al. 2012

Biopolymers

120

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Antibiotic resistance is an increasingly severe health problem. Antimicrobial peptides (AMPs) are being developed in order to overcome this problem, due lower bacterial resistance. Polymyxin B is an AMP with bactericidal effect on Gram-negative bacteria due to its high affinity for lipopolysaccharide (LPS). The objective of this work was to unravel the polymyxin B mechanisms of LPS neutralization and bactericidal activity. Using dynamic light scattering, it was observed that polymyxin B induces LPS aggregation in a concentration-dependent manner. The peptide increases the surface charge of LPS and membrane model systems, as revealed by zeta-potential measurements. The higher zeta-potential variations were detected in the presence of the negatively charged POPG membranes. This higher interaction with negatively charged membranes, made of POPG, was followed at higher peptide concentration by membrane permeabilization. Also, for zwitterionic POPC membranes a higher membrane leakage was detected. The peptide promotion of LPS aggregation may be related with the clearance of LPS from the bloodstream, eventually by facilitating macrophage phagocytosis and/ or blocking the binding of LPS to its receptor. Our data indicate that polymyxin B mechanism of action at the molecular level involves a first step of electrostatic approach toward LPS; then, it may be internalized and bind to the bacterial phosphatidylglycerol-rich membrane leaflets, inducing leakage at higher peptide concentrations.

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“…These sections were contrasted using a 4% uranyl acetate saturated solution and a 0.4% lead citrate aqueous solution (Ciena et al. ; Pícoli et al. ).…”

Section: Methodsmentioning

confidence: 99%

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

et al. 2013

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The agouti (Dasyprocta aguti Linnaeus, 1766) is a wild rodent belonging to the family Dasyproctidae that is found throughout Brazil and feeds on fruits and seeds. The aim of the present study was to describe the following features of the tongue of agouti: its morphological structures, the three-dimensional characteristics of the lingual papillae surface, the connective tissue cores (CTCs) and the epithelial cell ultrastructure. Four types of papillae were observed on the dorsal surface of the tongue with a triangular shape: filiform, fungiform, foliate and vallate. Filiform papillae were distributed throughout the tongue surface, and removal of the epithelial surface revealed conical CTCs and multifilaments. Fungiform papillae were observed in the rostral and middle regions, whereas foliate papillae developed in pairs on the lateral margin of the caudal region. Removal of the epithelium in these regions revealed CTCs with parallel laminar conformation. Vallate papillae were arranged in a V-shape in the caudal region, and their CTCs ranged in shape from elongate to ovoid. The ultrastructural components of the dorsal epithelium were the basal, spinous, granular and keratinised layers. A broad area with cytoplasmic projections was identified in the interface region between the lamina propria and the basal layer. Flattened cells with intermediate filaments were observed in the transitional region between spinous and granular layers. The keratinised layer was composed of superimposed epithelial cells where desmosomes and cell-surface microridges were observed. These structural features, including the three-dimensional aspects of the lingual papillae, the CTCs and the epithelial ultrastructure, indicate that when compared with other animals, particularly other rodent species, the morphological features of the tongue of agouti are relatively well developed, especially regarding foliate and vallate papillae.

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Ultrastructure of the myotendinous junction of the medial pterygoid muscle of adult and aged Wistar rats

Cited by 29 publications

References 24 publications

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Biophysical characterization of polymyxin b interaction with LPS aggregates and membrane model systems

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

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