Ultrastructure of the major ampullate gland of the black widow spider, Latrodectus hesperus

Casem, Merri Lynn; Tran, Lan Phuong P; Moore, Anne

doi:10.1016/s0040816602000836

Cited by 25 publications

(23 citation statements)

References 32 publications

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“…It has been reported that most Araneidae spiders (Tillinghast & Townley 1987; Moon 1998; Park & Moon 2002; Moon & An 2005) as well as Theridiidae spiders (Moon et al. 1998; Casem et al. 2002; Moon & Tillinghast 2002) use silk from the two kinds of ampullate glands for web building.…”

Section: Discussionmentioning

confidence: 99%

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Microstructure of the silk apparatus of the comb‐footed spider, Achaearanea tepidariorum (Araneae: Theridiidae)

Moon

2006

Entomological Research

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The microstructural organization of the silk‐spinning apparatus of the comb‐footed spider, Achaearanea tepidariorum, was observed by using a field emission scanning electron microscope. The silk glands of the spider were classified into six groups: ampullate, tubuliform, flagelliform, aggregate, aciniform and pyriform glands. Among these, three types of silk glands, the ampullate, pyriform and aciniform glands, occur only in female spiders. One (adult) or two (subadult) pairs of major ampullate glands send secretory ductules to the anterior spinnerets, and another pair of minor ampullate glands supply the median spinnerets. Three pairs of tubuliform glands in female spiders send secretory ductules to the median (one pair) and posterior (two pairs) spinnerets. Furthermore, one pair of flagelliform glands and two pairs of aggregate glands together supply the posterior spinnerets, and form a characteristic spinning structure known as a “triad” spigot. In male spiders, this combined apparatus of the flagelliform and the aggregate spigots for capture thread production is not apparent, instead only a non‐functional remnant of this triad spigot is present. In addition, the aciniform glands send ductules to the median (two pairs) and the posterior spinnerets (12–16 pairs), and the pyriform glands feed silk into the anterior spinnerets (90–100 pairs in females and 45–50 pairs in males).

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Section: Discussionmentioning

confidence: 99%

“…1972; Maretic 1987), whereas the silk of theridiids has been studied only in a few limited cases (Kovoor 1987; Moon et al. 1998; Casem et al. 2002; Moon & Tillinghast 2002).…”

Section: Introductionmentioning

confidence: 99%

Microstructure of the silk apparatus of the comb‐footed spider, Achaearanea tepidariorum (Araneae: Theridiidae)

Moon

2006

Entomological Research

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“…This leads us to suggest that shear forces are likely to play a greater role than salt effects during the fiber spinning process in vivo ( Casem et al, 2002, Knight and Vollrath, 2002, Perez-Rigueiro et al, 2001aand Perez-Rigueiro et al, 2001b.…”

Section: Figure Optionsmentioning

confidence: 99%

The role of salt and shear on the storage and assembly of spider silk proteins

Eisoldt

Hardy

Heim

et al. 2010

Journal of Structural Biology

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Major ampullate silk fibers of orb web-weaving spiders have impressive mechanical properties due to the fact that the underlying proteins partially fold into helical/amorphous structures, yielding relatively elastic matrices that are toughened by anisotropic nanoparticulate inclusions (formed from stacks of β-sheets of the same proteins). In vivo the transition from soluble protein to solid fibers involves a combination of chemical and mechanical stimuli (such as ion exchange, extraction of water and shear forces). Here we elucidate the effects of such stimuli on the in vitro aggregation of engineered and recombinantly produced major ampullate silk-like proteins (focusing on structurefunction relationships with respect to their primary structures), and discuss their relevance to the storage and assembly of spider silk proteins in vivo.

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“…[c]Spider silk shrin k to half its orig inal length & doubles in diameter (when in contact with water) called super contraction [22].…”

Section: Spinning Environment Control Materials Properties Of Fi Nal Smentioning

confidence: 99%

Spinning and Applications of Spider Silk

Singha¹,

Maity²,

Singha³

2012

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Spider silk is specially produced from the different species (sp.) of spider throughout the globe, specially the most fine and mechanically sound silk produced fro m nephila sp. The fibriller parallel model of spider silk gives higher mechanical properties with better Young's modulus and lower stress than normal silk coming fro m the silk-moth. In this review work we dicuss about the collection, composition, spinning and molecular structure, mechanical properties with its application of the spider silk.

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Ultrastructure of the major ampullate gland of the black widow spider, Latrodectus hesperus

Cited by 25 publications

References 32 publications

Microstructure of the silk apparatus of the comb‐footed spider, Achaearanea tepidariorum (Araneae: Theridiidae)

Microstructure of the silk apparatus of the comb‐footed spider, Achaearanea tepidariorum (Araneae: Theridiidae)

The role of salt and shear on the storage and assembly of spider silk proteins

Spinning and Applications of Spider Silk

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