Ultrastructure of the Human Stratum corneum

Corcuff, P.; Fiat, F.; Minondo, A.-M.

doi:10.1159/000056383

Cited by 22 publications

(9 citation statements)

References 13 publications

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“…These loose portions seem to constitute a minor barrier to water loss, but do represent a considerable mass when removed. This agrees with the heterogeneous nature of the SC structure [18][19][20][21]; where the SC compactum evolves into the SC disjunctum as the corneocytes migrate towards the surface, and progressively loose their corneodesmosome links. The cells of the SC disjunctum do not contribute significantly to the water barrier function of the SC.…”

Section: Resultssupporting

confidence: 83%

“…(3) yields an initial plateau followed by a declining straight line. It could be hypothesized that this initial plateau corresponds to the removal of the stratum corneum disjunctum; the looser outer stratum corneum layer [18][19][20][21] which does not provide a significant contribution to the barrier to water loss. It follows that its removal would contribute a significant mass change without a corresponding increase in TEWL.…”

Section: Introductionmentioning

confidence: 99%

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The determination of stratum corneum thickness An alternative approach

Russell¹,

Wiedersberg²,

Delgado‐Charro³

2008

European Journal of Pharmaceutics and Biopharmaceutics

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The individual thickness of the stratum corneum is required to normalize drug permeation profiles in dermatopharmacokinetic studies. The thickness is often estimated using tape-striping combined with transepidermal water loss measurements. A linear transformation of Fick's first law is used to relate the progressively thinner barrier with the corresponding increase in transepidermal water loss and to estimate the thickness by linear regression. However, the data from an important subset of subjects are poorly fitted to this linear model. This is typically due to the removal of loose outer layers of stratum corneum, which do not contribute significantly to barrier function. This work proposes two alternative non-linear models. All three models were used to fit data from 31 in vivo tape-striping experiments and their outcomes and goodness-of-fit compared. The results suggest that the linear model may overestimate the stratum corneum thickness and is open to subjectivity regarding the selection of data points to be fitted. The non-linear models satisfactorily fitted all the data, including all data points. No significant differences were found between the thicknesses derived from the two non-linear models. However, the analysis of the goodness-of-fit of the models to the data suggests a preference for a baseline-corrected approach.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

The determination of stratum corneum thickness An alternative approach

Russell¹,

Wiedersberg²,

Delgado‐Charro³

2008

European Journal of Pharmaceutics and Biopharmaceutics

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“…These beadlike structures are probably similar to the humplike structures described in a previous study (18) and may correspond to corneodesmosome or similar adhesive structures on the surface of corneocytes. This is supported by the fact that in transmission electron microscopy experiments, similar beadlike structures were found and attributed to intercellular junctions (33, 34). Corneodesmosomes are progressively degraded during the process of desquamation and thus are ultimately smaller in size at the surface – thus the smaller beadlike structures observed in the unstripped SC samples may correspond to partially degraded corneodesmosmes in the outer layers of the SC.…”

Section: Discussionmentioning

confidence: 55%

Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy

Rankl

Zhu

Luengo

et al. 2010

Experimental Dermatology

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Corneodesmosin, a protein known to be present in the stratum corneum (SC), plays an important role in its physical integrity. Here, a specific antibody to corneodesmosin was tethered via a flexible linker to an atomic force microscopy tip, and the interaction forces between this tip and the surface of the SC were successfully measured. Using the recently developed technique of simultaneous topography and recognition imaging, we were able to map the distribution of corneodesmosin on the surface of the SC at the nanoscale.

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“…Such studies have been previously carried out on various occasions using scanning electron microscopy (SEM) [7][8][9]. Despite the demands for highest quality, usually electron microscopic techniques show some deficiencies for the characterisation of the corneocyte surface structures [10].…”

Section: Introductionmentioning

confidence: 99%

Surface Ultra-Structure and Size of Human Corneocytes from Upper Stratum Corneum Layers of Normal and Diabetic Subjects with Discussion of Cohesion Aspects

Licht¹,

Heise²

2015

J Diabetes Metab

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Background: During the final stage of differentiation of cornified squamous epithelia like the human skin epidermis, anucleated corneocytes are formed. Formation of the horny layer and its ongoing desquamation are fundamental processes leading to the formation of an efficient epidermal barrier. Materials and methods:For a better understanding of the desquamation process, the role of corneocyte surface ultra-structure has been investigated using a special preparation technique for scanning electron microscopy (SEM). Human morphologically different corneocytes from the stratum corneum of the fingertip, the thenar eminence (thick skin), and the wrist below the carpus (thin skin) of normal and diabetic subjects were obtained by adhesive tape stripping. Results:The inside surface structure of corneocytes from thick skin shows prominent nubs, which are broader and more extended than those of thin skin. Towards their outside, corneocytes were flat with cavities as indentations of the nubs from neighboured cells providing clues on the mechanical strength of the 'intercellular stickiness'. As the size of thin skin corneocytes for diabetic subjects was also studied, it was found that their area was slightly nonlinearly dependent on age.Conclusion: Accordingly to a reduced proliferation and differentiation rate, as postulated for diabetic persons, differences in size were as expected but statistically not significant, compared with corneocytes under normal homeostasis conditions. For discussion is a model, for which the interwoven cellular connectivity provides additional mechanical strength for the stratum corneum in thick skin.

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Ultrastructure of the Human Stratum corneum

Cited by 22 publications

References 13 publications

The determination of stratum corneum thickness An alternative approach

The determination of stratum corneum thickness An alternative approach

Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy

Surface Ultra-Structure and Size of Human Corneocytes from Upper Stratum Corneum Layers of Normal and Diabetic Subjects with Discussion of Cohesion Aspects

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