Ultrastructure of the fresh water cyanobacterium<i>Anabaena variabilis</i>SPU 003 and its application for oxygen-free hydrogen production

Shah, Vishal; Garg, Nikki; Madamwar, Datta

doi:10.1111/j.1574-6968.2001.tb09448.x

Cited by 38 publications

References 13 publications

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Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges

Krishnan

Qian

Ananyev

et al. 2018

Synthetic Biology of Cyanobacteria

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With the demand for renewable energy growing, hydrogen (H) is becoming an attractive energy carrier. Developing H production technologies with near-net zero carbon emissions is a major challenge for the "H economy." Certain cyanobacteria inherently possess enzymes, nitrogenases, and bidirectional hydrogenases that are capable of H evolution using sunlight, making them ideal cell factories for photocatalytic conversion of water to H. With the advances in synthetic biology, cyanobacteria are currently being developed as a "plug and play" chassis to produce H. This chapter describes the metabolic pathways involved and the theoretical limits to cyanobacterial H production and summarizes the metabolic engineering technologies pursued.

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Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges

Krishnan

Qian

Ananyev

et al. 2018

Synthetic Biology of Cyanobacteria

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Ultrastructure of the cyanobacteriumNostoc muscorum and exploitation of the culture for hydrogen production

2003

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An effect of various physico-chemical parameters on nitrogenase-catalyzed oxygen-free hydrogen production by Nostoc muscorum was demonstrated. More hydrogen was produced in the light than in the dark. Optimum temperature was 40 degrees C Various sugars increased hydrogen production whereas on easily metabolized nitrogen sources it was inhibited. The production was sensitive to salinity and Fe3+, Cu2+, Zn2+ and Ni2+ ions. Ultrastructural study revealed many electron-dense layers outside the cell-wall area that have not been observed earlier.

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Studies on the factors affecting the growth and hemolytic activity of Anabaena variabilis

Wang

Xiu-kai

2007

J Appl Phycol

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The hemolytic activity of the cell-free culture supernatant of Anabaena variabilis OL S1 was investigated using the hemolysis of rabbit erythrocytes as an assay. The culture medium of A. variabilis started to exhibit hemolytic activity at the late exponential growth phase, and maximized at the stationary phase. The hemolytic toxin is heat-stable and can be extracted in dichloromethane. The hemolytic activities under different temperature, light intensity and pH showed a high correlation with the cell densities (r = 0.965, 0.951, 0.865, respectively), and the optimum condition is 28~30°C, pH 7.5~8.0, light intensity 120 μmol photons m −2 s −1 . The addition of 10~20 μg mL −1 chloramphenicol, an inhibitor of protein synthesis, exhibited no marked suppression on the hemolytic activity. The supplement of 1~20 μg mL −1 glycerol increased the hemolytic activity significantly, suggesting that synthesis of hemolysin was dependent on carbohydrate and lipid metabolism. The spectrum of erythrocyte sensitivity to the hemolysin indicated that rabbit erythrocytes were more sensitive to the hemolysin than were rat and human erythrocytes. Goldfish and cat erythrocytes were, however, insensitive to the hemolytic toxin of A. variabilis.

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Ultrastructure of the fresh water cyanobacteriumAnabaena variabilisSPU 003 and its application for oxygen-free hydrogen production

Cited by 38 publications

References 13 publications

Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges

Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges

Ultrastructure of the cyanobacteriumNostoc muscorum and exploitation of the culture for hydrogen production

Studies on the factors affecting the growth and hemolytic activity of Anabaena variabilis

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