Ultrastructure of the foliose lichen Myelochroa leucotyliza and its solo fungal and algal (Trebouxia sp.) partners

Arakawa, Satoko; Kanaseki, Takeshi; Wagner, Ralf; Goodenough, Ursula

doi:10.1016/j.algal.2021.102571

Cited by 3 publications

(2 citation statements)

References 108 publications

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“…Eisosomes are defined as plasma membrane invaginations filled with protein complexes that are thought to be involved in the desiccation-dehydration processes in eukaryotic organisms with cell walls [ 87 ]. These structures have been described for Trebouxia cells analyzed within the lichen thallus [ 87 , 97 , 99 ], and their detection serves as a further confirmation of the desiccation-dehydration tolerance typical of lichens and their phycobionts in particular (e.g., [ 100 , 101 ]).…”

Section: Discussionmentioning

confidence: 59%

“…More advanced techniques have been also applied to better investigate the cellular structure: recently, it was shown that fast-freeze electron microscopy techniques (such as cryo-SEM) capture organisms at high resolution in their living state, offering novel views of the cellular ultrastructure, organization, and differentiation [ 95 , 96 , 97 ]. Although these techniques bypass possible ultrastructure modifications due to preparation procedures, they have not been extensively used with lichens and their axenically isolated phycobionts [ 96 , 98 ].…”

Section: Discussionmentioning

confidence: 99%

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Trebouxia lynnae sp. nov. (Former Trebouxia sp. TR9): Biology and Biogeography of an Epitome Lichen Symbiotic Microalga

Barreno

Muggia

Chiva

et al. 2022

Biology

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Two microalgal species, Trebouxia jamesii and Trebouxia sp. TR9, were detected as the main photobionts coexisting in the thalli of the lichen Ramalina farinacea. Trebouxia sp. TR9 emerged as a new taxon in lichen symbioses and was successfully isolated and propagated in in vitro culture and thoroughly investigated. Several years of research have confirmed the taxon Trebouxia sp. TR9 to be a model/reference organism for studying mycobiont–photobiont association patterns in lichen symbioses. Trebouxia sp. TR9 is the first symbiotic, lichen-forming microalga for which an exhaustive characterization of cellular ultrastructure, physiological traits, genetic and genomic diversity is available. The cellular ultrastructure was studied by light, electron and confocal microscopy; physiological traits were studied as responses to different abiotic stresses. The genetic diversity was previously analyzed at both the nuclear and organelle levels by using chloroplast, mitochondrial, and nuclear genome data, and a multiplicity of phylogenetic analyses were carried out to study its intraspecific diversity at a biogeographical level and its specificity association patterns with the mycobiont. Here, Trebouxia sp. TR9 is formally described by applying an integrative taxonomic approach and is presented to science as Trebouxia lynnae, in honor of Lynn Margulis, who was the primary modern proponent for the significance of symbiosis in evolution. The complete set of analyses that were carried out for its characterization is provided.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Trebouxia lynnae sp. nov. (Former Trebouxia sp. TR9): Biology and Biogeography of an Epitome Lichen Symbiotic Microalga

Barreno

Muggia

Chiva

et al. 2022

Biology

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Enhanced wastewater treatment performance by understanding the interaction between algae and bacteria based on quorum sensing

Liu

Feng

Sheng

et al. 2022

Bioresource Technology

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Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of Trebouxia lynnae (Trebouxiophyceae, Lichenized Microalgae)

et al. 2023

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The lichenized green microalga Trebouxia lynnae Barreno has been recently described and is considered a model organism for studying lichen chlorobionts. Its cellular ultrastructure has already been studied in detail by light, electron, and confocal microscopy, and its nuclear, chloroplast and mitochondrial genomes have been sequenced and annotated. Here, we investigated in detail the ultrastructure of in vitro grown cultures of T. lynnae observed by Low Temperature Scanning Electron Microscopy (LTSEM) applying a protocol with minimum intervention over the biological samples. This methodology allowed for the discovery of ultrastructural features previously unseen in Trebouxiophyceae microalgae. In addition, original Transmission Electron Microscopy (TEM) images of T. lynnae were reinterpreted based on the new information provided by LTSEM. The nucleolar vacuole, dictyosomes, and endoplasmic reticulum were investigated and reported for the first time in T. lynnae and most likely in other Trebouxia lineages.

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Ultrastructure of the foliose lichen Myelochroa leucotyliza and its solo fungal and algal (Trebouxia sp.) partners

Cited by 3 publications

References 108 publications

Trebouxia lynnae sp. nov. (Former Trebouxia sp. TR9): Biology and Biogeography of an Epitome Lichen Symbiotic Microalga

Trebouxia lynnae sp. nov. (Former Trebouxia sp. TR9): Biology and Biogeography of an Epitome Lichen Symbiotic Microalga

Enhanced wastewater treatment performance by understanding the interaction between algae and bacteria based on quorum sensing

Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of Trebouxia lynnae (Trebouxiophyceae, Lichenized Microalgae)

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