Ultrastructure of the Articular Cartilage After Systemic Administration of Hydrocortisone in the Rabbit

Higuchi, Masanori; Masuda, Takeshi; Susuda, Kouichi; Ishii, Seiichi; ABE, KAZUHIRO

doi:10.1097/00003086-198010000-00037

Cited by 12 publications

(3 citation statements)

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“…The findings of fibrillation in rats given corticosteroids were consistent with those in baboons, where the condylar cartilage attained a grossly fibrillated appearance after biweekly intra-articular injections of 20 mg of glucocorticoids (10). Findings obtained by quantitative electron microscopy indicate that lower daily doses (1-5 mg/kg body weight) of steroids administered intramuscularly also induce degenerative changes of the chondrocytes, including pyknotic nuclei and reduced rough endoplasmic reticula (24). According to WEISS et al (11), glucocorticoids interfere with the anabolic activities of cartilage cells by inhibiting cartilage cell prohferation, by reducing RNA and protein synthesis, and by decreasing the synthesis of proteoglycans.…”

Section: Discussionmentioning

confidence: 99%

Surface structure of the temporomandibular joint in normal and steroid‐treated rats: a scanning electron microscopic study

Rohlin

Hallberg

Näsström³

et al. 1993

European J Oral Sciences

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The aim was to study the ultrastructure of the surface of the temporomandibular joint (TMJ) in mature rats. The TMJs from control rats and rats given corticosteroids for 10 days or 38 days were examined. In three joints from the control rats the disk was detached from the condyle before preparation and analysis. Scanning electron microscopy was performed on the condyle, the disk, and the temporal component. Generally, the surface of the three components was predominantly smooth, although the condyle exhibited a more even surface than the disk and the temporal component. In the fossa a pitted or ridged appearance was observed in some areas. There was a striking difference between the surface structure of disks attached to, and that of disks detached from, the condyle during preparation. A prominent undulation of surface was evident in disks detacted from the condyle. Below the surface layer of the articular cartilage, a network of collagen fibers and fibrils running in all directions could be observed in all three components. In limited areas there was fibrillation and shallow defects of the surface layer. These changes were seen in all rats given corticosteroids for 38 days but also in some rats given corticosteroids for 10 days and in a few control rats.

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Section: Discussionmentioning

confidence: 99%

Surface structure of the temporomandibular joint in normal and steroid‐treated rats: a scanning electron microscopic study

Rohlin

Hallberg

Näsström³

et al. 1993

European J Oral Sciences

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“…Most early in vivo experiments showed that corticosteroids, especially after intra-articular administration, may provoke or worsen cartilage lesions. [1][2][3][4][5][6][7][8][9] However, a systematic review of these reports shows that degeneration of articular cartilage, resulting from steroid administration, occurred after the administration of unrealistically high doses of this hormone. Similarly, Charcot-like arthropathy has been reported in man after a series of about 20 intra-articular injections of steroids.…”

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Physiological levels of hydrocortisone maintain an optimal chondrocyte extracellular matrix metabolism

Wang

Elewaut²,

Hoffman³

et al. 2004

Annals of the Rheumatic Diseases

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Objective: To investigate the effects of physiological doses of hydrocortisone on synthesis and turnover of cell associated matrix (CAM) by human chondrocytes obtained from normal articular cartilage. Methods: Human articular cartilage cells were obtained from visually intact cartilage of the femoral condyles of five donors and maintained in culture for one week to reach equilibrium in accumulated CAM compounds. 0, 0.05, 0.20, and 1.0 mg/ml hydrocortisone was added to the nutrient media during the entire culture period. Cells were liberated and levels of CAM aggrecan, type II collagen, and fibronectin, of intracellular IGF-1, IL1a and b, and of their respective plasma membrane bound receptors IGFR1, IL1RI, and the decoy receptor IL1RII, were assayed by flow cytometry. Results: In comparison with controls, hydrocortisone treated chondrocytes, at all concentrations, expressed significantly higher plasma membrane bound IGFR1. Intracellular IGF-1 levels remained unchanged. Together with these changes, reflecting an increased ability to synthesise extracellular matrix (ECM) macromolecules, hydrocortisone treated cells expressed significantly higher amounts of the plasma membrane bound decoy IL1RII. Concurrently, intracellular IL1a and b levels and membrane bound IL1RI were down regulated. Levels of CAM aggrecan, type II collagen, and fibronectin were significantly up regulated in the chondrocytes treated with hydrocortisone. Conclusion: 0.05 mg/ml hydrocortisone treated chondrocytes had decreased catabolic signalling pathways and showed an enhanced ability to synthesise ECM macromolecules. Because IL1 activity was decreased and the expression of IL1RII decoy receptor enhanced, more of the ECM macromolecules produced remained accumulated in the CAM of the chondrocytes. The effects were obtained at doses comparable with physiological plasma levels of hydrocortisone in humans.

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“…Chandler et al ( 1958aChandler et al ( , 1958b studied the effects of intraarticular injections of corticosteroids on articular cartilage and observed that in a number of cases, the functional improvement was accompanied by excessive and accelerated destruction of the joints. It is now generally accepted that local and systemic administration of corticosteroids affect the structure and function of the articular cartilage (Behrens 1975and 1976, Higuchi et al 1980, Mankin and Conger 1966, Shaw and Lacey 1973. In addition, local administration of hydrocortisone was also found to produce destructive changes in the meniscus.…”

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confidence: 99%

The effect of intraarticular hydrocortisone injection on the articular cartilage of rabbits

Papacrhistou

Anagnostou

Katsorhis

1997

Acta Orthopaedica Scandinavica

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Ultrastructure of the Articular Cartilage After Systemic Administration of Hydrocortisone in the Rabbit

Cited by 12 publications

References 0 publications

Surface structure of the temporomandibular joint in normal and steroid‐treated rats: a scanning electron microscopic study

Surface structure of the temporomandibular joint in normal and steroid‐treated rats: a scanning electron microscopic study

Physiological levels of hydrocortisone maintain an optimal chondrocyte extracellular matrix metabolism

The effect of intraarticular hydrocortisone injection on the articular cartilage of rabbits

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