Ultrastructure of Taste Receptors

Murray, Raymond G.

doi:10.1007/978-3-642-65245-5_2

Cited by 71 publications

(85 citation statements)

References 32 publications

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“…From the four types of cells described in mammalian taste buds (basal cell, type I or dark cell, type II or light cell, and perigemmal cell), type I (representing 50-70% of the cells) are believed to have receptor functions (Farbman, 1965;Murray, 1971). In our study the transient Na current was present in 72% of the cells patched.…”

Section: Characteristics Of the Trcsmentioning

confidence: 57%

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Membrane currents in taste cells of the rat fungiform papilla. Evidence for two types of Ca currents and inhibition of K currents by saccharin.

Béhé

DeSimone

Avenet

et al. 1990

The Journal of General Physiology

171

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Taste buds were isolated from the fungiform papilla of the rat tongue and the receptor cells (TRCs) were patch clamped. Seals were obtained on the basolateral membrane of 281 TRCs, protruding from the intact taste buds or isolated by micro-dissection. In whole-cell configuration 72% of the cells had a TTX blockable transient Na inward current (mean peak amplitude 0.74 hA). All cells had outward K currents. Their activation was slower than for the Na current and a slow inactivation was also noticeable. The K currents were blocked by tetraethylammonium, Ba, and 4-aminopyridine, and were absent when the pipette contained Cs instead of K. With 100 mM Ba or 100 mM Ca in the bath, two types of inward current were observed. An L-type Ca current (ICaL) activated at -20 mV had a mean peak amplitude of 440 pA and inactivated very slowly. At 3 mM Ca the activation threshold of ICa L was near -40 mV. A transient T-type current (/CAT) activated at -50 mV had an average peak amplitude of 53 pA and inactivated with a time constant of 36 ms at -30 inV. ICR L was blocked more efficiently by Cd and D600 than/Ca T. ICa T was blocked by 0.2 mM Ni and half blocked by 200 #M amiloride. In whole-cell voltage clamp, Na-saccharin caused (in 34% of 55 cells tested) a decrease in outward K currents by 21%, which may be expected to depolarize the TRCs. Also, Na-saccharin caused some taste cells to fire action potentials (on-ceLl, 7 out of 24 cells; whole-ceLl, 2 out of 38 cells responding to saccharin) of amplitudes sufficient to activate/CAL. ThUS the action potentials will cause Ca inflow, which may trigger release of transmitter.

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Section: Characteristics Of the Trcsmentioning

confidence: 57%

“…The onion-shaped taste bud of the rat, 30-50 t~m in diameter, contains 50-100 cells, 50-70% of which are TRCs (Farbman, 1965;Murray, 1971). We developed a protocol that allowed the buds to retain their shape when isolated from the tongue epithelium.…”

Section: Taste Cell Preparationmentioning

confidence: 99%

Membrane currents in taste cells of the rat fungiform papilla. Evidence for two types of Ca currents and inhibition of K currents by saccharin.

Béhé

DeSimone

Avenet

et al. 1990

The Journal of General Physiology

171

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“…The result of a number of investigations into the ultrastructure of taste buds in some animal species (Beidler and Smallman 1965;De Lorenzo 1958;Farbman 1965a, Farbman and Tonkers 1971;Fujimoto and Murray 1970;Murray 1969Murray , 1971Murray , 1973Murray and Murray 1960, 1967Murray, Murray and Fujimoto 1969;Takeda and Hoshino 1975;Uga 1966Uga , 1969 and others) contributed significantly to the understanding of taste perception mechanisms. They provided grounds for the development of methods for studying biochemistry and physiology of taste perception (Beidler 1970;De Han and Graziadei 1971;Hirata and Nada 1975;Spoendlin 1970;Takeda 1976; Beauchamp and Cowart 1986, and others).…”

Section: Abstract Tichy F: the Morphogenesis Of Circumvallate Papillmentioning

confidence: 99%

The Morphogenesis of Circumvallate Papillae and the Differentiation of Taste Buds in the Porcine Foetus from Day 76 till Birth and in the Adult Pig

Tichý¹

1991

Acta Vet. Brno

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Tichy F.: The Morphogenesis of Circumvallate Papillae and the Differentiation of Taste Buds in the Porcine Foetusfrom Day 76 till Birth and in the Adult Pig. Acta vet. Brno, 60, 1991: 307-315.The development and morphology of some lingual structures were studied in the tongues of porcine foetuses at 76, 81 and 94 days after fertilization, of piglets 2 days after birth and of adult pigs. Special attention was given to the morphogenesis of circumvallate papillae and the differentiation of taste buds in papillary epithelium. The formation of the other types of papillae, and the development of the lamina propria mucosae and the related structures were also investigated.Taste buds prevailed in the wall epithelium of the circumvallate papilla, while in its dorsal surface they occurred only occasionally during the first stages of the period studied. The amount of taste buds increased gradually in foliate papillae. In the period under study taste buds showed distinct differentiation of their cells.The porus gustatorius formed immediately after the taste bud reached the surface of epithelium."Secondary" ducts of the gll. gustatoriae observed in circumvallate papillae in the late prenatal periods were present till birth.The development of the other lingual papillae followed that of circumvallate papillae. Tongue, papillae, taste bud, development, swineThe structure and composition of lingual mucosa is well understood at the levels of both light and electron microscopy. The basic information on the structure of the taste organ, based on electron microscopic observations, in laboratory animals as well as in man was reported early in this century (Kolmer 1910;Retzius .1912;Heidenhain 1914).The result of a number of investigations into the ultrastructure of taste buds in some animal species (Beidler and Smallman 1965;De Lorenzo 1958;Farbman 1965a, Farbman and Tonkers 1971;Fujimoto and Murray 1970;Murray 1969Murray , 1971Murray , 1973Murray and Murray 1960, 1967Murray, Murray and Fujimoto 1969;Takeda and Hoshino 1975;Uga 1966Uga , 1969 and others) contributed significantly to the understanding of taste perception mechanisms. They provided grounds for the development of methods for studying biochemistry and physiology of taste perception (Beidler 1970;De Han and Graziadei 1971;Hirata and Nada 1975;Spoendlin 1970;Takeda 1976; Beauchamp and Cowart 1986, and others).Attention has also been paid to the development of the taste organ in man and various mammalian species (Bredler and Smallman 1965;Farbman 1965bFarbman , 1971 Takeda 1972 and others).Less information is available on the development of mucosal structures of the tongue in relation to periods of development in farm animals. Particularly poor are data on the time of commencement of taste buds and on the changes in the gustatory receptor occurring during ontogenesis.

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“…The observations so far reported suggest that differences in structure are related to species specificity, the localization of the bud in lingual epithelium (Beidler 1969;Murray 1971Murray , 1973Mattern and Paran 1974)orotherfactors acting on the taste bud in the oral cavity.…”

Section: Taste Bud Porus Gustatorius Nerve Fibre Domestic Cat Ultmentioning

confidence: 99%

The ultrastructure of taste buds in the newborn cat

Tichý¹

1994

Acta Vet. Brno

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Tichy F. : The ultrastructure of taste buds in the newborn cat. Acta vet. Bmo, 63, 1994: 49-54.The ultrastructure of taste buds on circumvallate papillae was studied in one-day-old kittens. Attention was paid to the occurrence, localization and presence of organelles in dark, light and receptor cells, formation of the porus gustatorius and occurence of intragemmal nerve fibres and their relation to the three cell types of the bud.At the time after birth, the feline taste bude contained dark, light and receptor cells. All three cell types had a high amount of filaments in the cytoplasm. In the dark cells, secretory granules and dark substance in the porus gustatorius were seen only occasionally. Both dark and light vesicles in the cytoplasm were only rare findings in the receptor cells. The apical portions of bud cells did not terminate as microvilli and never came to level with the epithelial surface. Infrequent intragemmal nerve fibres were in contact with all bud cell types.Taste bud, porus gustatorius, nerve fibre, domestic cat, ultrastru~ture

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Ultrastructure of Taste Receptors

Cited by 71 publications

References 32 publications

Membrane currents in taste cells of the rat fungiform papilla. Evidence for two types of Ca currents and inhibition of K currents by saccharin.

Membrane currents in taste cells of the rat fungiform papilla. Evidence for two types of Ca currents and inhibition of K currents by saccharin.

The Morphogenesis of Circumvallate Papillae and the Differentiation of Taste Buds in the Porcine Foetus from Day 76 till Birth and in the Adult Pig

The ultrastructure of taste buds in the newborn cat

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