Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Willms, Kaethe; Robert, Lilia; Jiménez, José Agustín; Everhart, Mary E.; Kuhn, Raymond

doi:10.1007/s00436-004-1125-5

Cited by 24 publications

(41 citation statements)

References 20 publications

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“…centripunctata, see Bâ and Marchand 1994b). Similar structures, electron-dense rods, were observed in the dilepidid D. undula (see OEwiderski et al 2000) and in the species of the genus Taenia (see Tian et al 1998, Ndiaye et al 2003a, Willms et al 2004.…”

Section: Spermatozoonsupporting

confidence: 57%

“…The spermatozoon of A. beema has a single crested body, as it is in the cyclophyllidean families Taeniidae (see Miquel et al 2000, Ndiaye et al 2003a, Willms et al 2004, Mesocestoididae (see Miquel et al 1999), Nematotaeniidae (see Mokhtar-Maamouri and Azzouz-Draoui 1990), Dilepididae (see OEwiderski et al 2000OEwiderski et al , 2002, Dipylidiidae (see , Ndiaye et al 2003b, Miquel et al 2005a and Metadilepididae (see Yoneva et al 2006). Two families, Catenotaeniidae and Davaineidae, are characterised by the presence of two crested bodies on the mature spermatozoon Hidalgo et al 2000;Bâ and Marchand 1994c;Bâ et al 2005a, b).…”

Section: Spermatozoonmentioning

confidence: 99%

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Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

Yoneva

Miquel

Świderski³

et al. 2006

Acta Parasitologica

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The ultrastructure of the spermiogenesis of a dilepidid cestode species is described for the first time. The spermiogenesis of Angularella beema is characterised by absence of both flagellar rotation and proximodistal fusion. The differentiation zone is surrounded by cortical microtubules and is delimited by a ring of arching membranes. It contains two centrioles, one of which develops the axoneme that grows directly into the elongating cytoplasmic protrusion. This pattern of spermiogenesis was described as the Type IV spermiogenesis of cestodes. Among cestodes, similar pattern of spermiogenesis is known in the family Hymenolepididae and in some representatives of the family Anoplocephalidae. The mature spermatozoon of A. beema consists of five regions differing in their ultrastructural characteristics. It is characterised by the presence of cortical microtubules (spirally arranged at angle of 30-40° to the spermatozoon axis) and a single crested body. There is a periaxonemal sheath in certain parts of the spermatozoon as well as glycogen-like granules between the periaxonemal sheath and the cortical microtubules. The comparisons of the mature spermatozoon of A. beema with those of other two dilepidid species (Dilepis undula and Molluscotaenia crassiscolex) demonstrate some variation within the family: presence of periaxonemal sheath in A. beema and D. undula and its absence in M. crassiscolex; presence of electron-dense rods in D. undula and their absence in A. beema.

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Section: Spermatozoonsupporting

confidence: 57%

Section: Spermatozoonmentioning

confidence: 99%

Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

Yoneva

Miquel

Świderski³

et al. 2006

Acta Parasitologica

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“…This is the case of the catenotaeniid Catenotaenia pusilla, the davaineids, the dipylidiids, the metadilepidid Skrjabinoporus merops, the nematotaeniid Nematotaenia chantalae, and the taeniids (Featherston 1971;Mokhtar-Maamouri and Azzouz-Draoui 1990;Bâ and Marchand 1994b;Miquel et al , 2005bMiquel et al , 2009bHidalgo et al 2000;Ndiaye et al 2003a, b;Willms et al 2003Willms et al , 2004Yoneva et al 2006b). In the present study, we demonstrate that T. taeniaeformis also exhibits Type 3 spermiogenesis.…”

Section: Spermiogenesismentioning

confidence: 99%

“…At present, there are ultrastructural studies on spermiogenesis or sperm of the following nine species of the Taeniidae: Echinococcus granulosus, E. multilocularis, Taenia crassiceps, T. hydatigena, T. mustelae, T. parva, T. pisiformis, T. saginata and T. solium (Morseth 1969;Featherston 1971;Barrett and Smyth 1983;Shi et al 1994;Tian et al 1998;Miquel et al 2000;Ndiaye et al 2003a;Willms et al 2003Willms et al , 2004. The purpose of the present paper is to describe the ultrastructure of spermatogenesis of Taenia taeniaeformis with emphasis on the early stages comprising 4 generations of spermatogonia, primary and secondary spermatocytes, spermatids and the entire process of sperm differentiation or spermiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of spermatogenesis of Taenia taeniaeformis (Batsch, 1786) (Cestoda, Cyclophyllidea, Taeniidae) and comparison of spermatological characters in the family Taeniidae Ludwig, 1886

Miquel

Świderski²,

Foronda³

et al. 2009

Acta Parasitologica

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The ultrastructure of spermatogenesis of Taenia taeniaeformis is described for the first time by means of transmission electron microscopy (TEM). Mature testes contain all stages of spermatogenesis; primary spermatogonia are usually situated at the periphery and mature spermatozoa in the centre of testes. The general process is similar to that described in other cestodes. Six incomplete, synchronic cytokineses occur: four mitotic and two meiotic cell divisions. All these divisions occur simultaneously, resulting in a rosette cluster of four tertiary spermatogonia, then eight quaternary spermatogonia, and subsequently sixteen primary spermatocytes. All of these enter into a growth period and their enlarged nuclei move to the periphery of cells of the rosettes. The first meiotic division forms thirty-two secondary spermatocytes and after the second meiotic division, there are sixty-four spermatids. Spermiogenesis in T. taeniaeformis corresponds to the Bâ and Marchand's Type 3 and begins with the formation of a differentiation zone in the form of a conical projection of cytoplasm delimited by a ring of arching membranes and surrounded by submembranous cortical microtubules. Within this area, there are two centrioles, orthogonally disposed, and vestigial striated rootlets. Only one of the centrioles develops a flagellum that grows externally to the cytoplasmic extension. Posteriorly, a flagellar rotation inferior to 90º occurs and the flagellum becomes parallel to the cytoplasmic extension. Later, the two processes fuse during the so-called proximodistal fusion. The nucleus elongates and moves into the cytoplasmic extension. In the final stage of spermiogenesis, a single crested body appears at the base of the differentiating spermatozoon. Finally, the ring of arching membranes constricts and the young spermatozoon detaches from the residual cytoplasm. Ultrastructural aspects of spermatogenesis are compared with that of other cestodes studied to date, particularly of the family Taeniidae.

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“…It has also been widely demonstrated that a number of parasites induce apoptosis in their host tissues (Moore and Matlashewshi 1994;Ragland et al 1994;Lopez et al 1995;Toure-Blade et al 1996) Invertebrate tissues of cestoda have very high reproductive capacities (Conn 1993) and are therefore expected to exhibit apoptosis in tissues which are undergoing constant growth and differentiation. The purpose of this paper is to examine the process of PCD in the adult stage of two experimental taeniids, Taenia solium and Taenia crassiceps, grown in the immunosuppressed golden hamster model (Sato and Kamiya 1989;Sato et al 1994;Maravilla et al 1998;Willms et al 2003;Willms et al 2004). …”

Section: Introductionmentioning

confidence: 99%

Apoptosis patterns in experimental Taenia solium and Taenia crassiceps strobilae from golden hamsters

et al. 2005

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Apoptosis or programmed cell death (PCD) patterns of two taeniid species, Taenia solium and Taenia crassiceps, were explored in adult tapeworms grown in golden hamsters. Animals were fed either ten viable T. solium cysticerci from naturally infected pigs or from T. crassiceps WFU strain maintained in Balb/c mice. Adult strobilae were recovered from the intestine at different times after infection and either frozen at -70 degrees C or fixed in paraformaldehyde-glutaraldehyde. Frozen sections were processed using the DNA fragmentation fluorescent TUNEL reagents and examined in an epifluorescent microscope. Fixed tissues were processed for light and electron microscopy. Typical apoptotic cells were found in the central core of scolex and strobilar tissues, mainly in the germinal tissue and subtegumentary areas. By the TUNEL technique, cells exhibited the characteristic fluorescent images of condensed nuclear chromatin. By light microscopy of thick sections stained with toluidine blue, we found a number of small rounded cells which had lost their cytoplasmic bridges and had shrunken nuclei with aggregated chromatin, cells which were found interspersed with normal syncytial cells. Similar cell morphology was confirmed by electron microscopy. Stunted viable worms, recovered with longer mature specimens, had very short strobilae and exhibited a large number of apoptotic cells in the germinal neck tissues. The results are consistent with the syncytial nature of these parasites, and strongly suggest that cell proliferation and PCD in these adult cestodes are continuous processes of the germinal tissue and tegumentary cytons.

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Ultrastructure of spermiogenesis and the spermatozoon in Taenia crassiceps strobilae WFU strain (Cestoda, Cyclophyllidea, Taeniidae) from golden hamsters

Cited by 24 publications

References 20 publications

Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

Ultrastructure of spermatogenesis of Taenia taeniaeformis (Batsch, 1786) (Cestoda, Cyclophyllidea, Taeniidae) and comparison of spermatological characters in the family Taeniidae Ludwig, 1886

Apoptosis patterns in experimental Taenia solium and Taenia crassiceps strobilae from golden hamsters

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