Ultrastructure of rat odontoblasts in various stages of their development and maturation

Takuma, S.; Nagai, Noriyuki

doi:10.1016/0003-9969(71)90205-6

Cited by 140 publications

(70 citation statements)

References 8 publications

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“…The unlabelled cells could represent replacement odontoblasts derived from a population of G,-blocked, predetermined cells that responded to the inducing event by entering M-phase directly and thus not incorporating [3H]-thymidine. Such a mechanism has been proposed by Stanley (1962), Takuma and Nagai (1971), and Slavkin (1974). Cotton (1968) and Tomeck and Wagner (1980) found no labelled odontoblast-like cells after tooth grinding without exposure and labelling with [3H]-thymidine.…”

Section: Discussionmentioning

confidence: 81%

“…The existence of a G,-blocked, predetermined population of odontoblast replacement cells was suggested by Stanley (1962), Takuma and Nagai (1971), and Slavkin (1974) because of the similar morphology of cells in the cell-rich zone and the adjacent functioning, mature odontoblasts. Findings by Cotton (1968) and Torneck and Wagner (1980) seem to support this theory: they found no [3H]-thymidine-labelled odontoblast-like cells in rat molar pulps after tooth grinding without pulp exposure, which suggests that odontoblasts destroyed by the grinding were replaced by predetermined cells that did not replicate their DNA in response to the injury.…”

mentioning

confidence: 96%

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Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth

Fitzgerald

Chiego

Heys

1990

Archives of Oral Biology

200

143

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Summary-Cellmigration and replication associated with odontoblast replacement occurring soon after pulp exposure in primate teeth were studied. Class 5 cavity preparations resulting in pulp exposures were restored with a calcium hydroxide-containing capping agent and amalgam. Eighty-four and 96 h after this the animals were injected with 0.5 pCi/g body wt tritiated thymidine (sp. act. 6.7 Ci/mM). Teeth were extracted 6, 8, 10 and 12 days after treatment. The number of labelled cells as well as the number of grains per labelled cell were counted for odontoblast-like, fibroblast-like and perivascular cells in three 60 x 260 pm zones. These zones represented the odontoblast and cell-free (zone l), cell-rich (zone 2) and deep pulp (zone 3) areas of normal pulp tissue. Ten sections centred around the mid-point of the exposure were counted for each tooth. Matrix formation and labelled odontoblast-like cells were observed at the interface between the capping agent and the pulp as early as day 8. Other significant findings were: (1) an increase In labelled odontoblast-like cells in zone 1 over time, suggesting a continual influx of differentiating cells; (2) an increase in labelled cells in zone 1 over time with a concurrent decrease in zone 3, suggesting that the influx of cells in zone 1 was from the deeper pulp; and (3) differences in grain counts between zones, treatment times and cell types, indicating that at least two DNA replications had occurred between initial treatment and final odontoblast-like cell differentiation.

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Section: Discussionmentioning

confidence: 81%

mentioning

confidence: 96%

Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth

Fitzgerald

Chiego

Heys

1990

Archives of Oral Biology

200

143

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“…The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or the small integrin binding ligand, N-linked glycoprotein (SIBLING) family, which is composed of dentin sialophosphoprotein (Dspp), dentin matrix protein 1 (Dmp1), osteopontin (Opn), bone sialoprotein (Bsp), and matrix extracellular phosphoglycoprotein (Mepe) (16). Dspp is proteolytically cleaved into dentin sialoprotein (Dsp) and dentin phosphoprotein (Dpp) (49,55).…”

mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

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“…When these reports are considered together, it appears that the odontoblast lacks true desmosomes. Although "desmosomes" or "desmosome-like junctions" in the odontoblast have been described on the basis of electron microscopy (5,12,13,22), it should be noted that their ultrastructure is different from that of the desmosomes in epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Immunofluorescent localization of myosin, alpha-actinin and tropomyosin in odontoblast microfilament bundles of the rat incisor.

Nishikawa

Sasa

1989

Cell Struct. Funct.

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ABSTRACT.The localizatioin of alpha-actinin, tropomyosin, myosin and actin in odontoblasts was examined by fluorescence microscopy using well characterized antibodies and rhodamine-phalloidin. All the reagents labeled the distal end of the cell body in the form of an oval ring with a preferential axis along the tooth axis. This ring was often interrupted. In conventional electron microscopy, microfilament bundles with periodical dense spots were running along the tooth axis at the level of the distal end of the cell body. The periodicity was about 0.6-1.0 µm. It may be possible that this dynamic structure functions to keep odontoblasts in a layer by contracting in an isometric form.

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Ultrastructure of rat odontoblasts in various stages of their development and maturation

Cited by 140 publications

References 8 publications

Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth

Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Immunofluorescent localization of myosin, alpha-actinin and tropomyosin in odontoblast microfilament bundles of the rat incisor.

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