Ultrastructure of mouse egg-cylinder

Solter, Davor; Damjanov, Ivan; Škreb, N.

doi:10.1007/bf00569266

Cited by 47 publications

(20 citation statements)

References 5 publications

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“…With the exception of the DVE, which is morphologically distinct from the EVE, the EVE is characterized by its flat squamous cells, largely without microvilli or apical vacuoles. TVE is cuboidal containing some microvilli and vacuoles, and XVE is columnar with many microvilli and apical vacuoles (Bonnevie, 1950;Enders et al, 1978;Hogan and Tilly, 1981;Solter et al, 1970).…”

Section: Development Of the Mouse Vementioning

confidence: 99%

The hypoblast (visceral endoderm): an evo-devo perspective

2012

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SummaryWhen amniotes appeared during evolution, embryos freed themselves from intracellular nutrition; development slowed, the mid-blastula transition was lost and maternal components became less important for polarity. Extra-embryonic tissues emerged to provide nutrition and other innovations. One such tissue, the hypoblast (visceral endoderm in mouse), acquired a role in fixing the body plan: it controls epiblast cell movements leading to primitive streak formation, generating bilateral symmetry. It also transiently induces expression of pre-neural markers in the epiblast, which also contributes to delay streak formation. After gastrulation, the hypoblast might protect prospective forebrain cells from caudalizing signals. These functions separate mesendodermal and neuroectodermal domains by protecting cells against being caught up in the movements of gastrulation.

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Section: Development Of the Mouse Vementioning

confidence: 99%

The hypoblast (visceral endoderm): an evo-devo perspective

2012

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“…1A). VE cells in contact with extra-embryonic ectoderm are more columnar and cuboidal, while VE cells covering the epiblast are flatter and more epithelial in shape (Solter et al, 1970). They also exhibit differences in gene expression.…”

Section: Introductionmentioning

confidence: 99%

Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts

et al. 2005

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The extra-embryonic endoderm lineage plays a major role in the nutritive support of the embryo and is required for several inductive events, such as anterior patterning and blood island formation. Blastocyst-derived embryonic stem (ES) and trophoblast stem (TS) cell lines provide good models with which to study the development of the epiblast and trophoblast lineages,respectively. We describe the derivation and characterization of cell lines that are representative of the third lineage of the blastocyst –extra-embryonic endoderm. Extra-embryonic endoderm (XEN) cell lines can be reproducibly derived from mouse blastocysts and passaged without any evidence of senescence. XEN cells express markers typical of extra-embryonic endoderm derivatives, but not those of the epiblast or trophoblast. Chimeras generated by injection of XEN cells into blastocysts showed exclusive contribution to extra-embryonic endoderm cell types. We used female XEN cells to investigate the mechanism of X chromosome inactivation in this lineage. We observed paternally imprinted X-inactivation, consistent with observations in vivo. Based on gene expression analysis, chimera studies and imprinted X-inactivation, XEN cell lines are representative of extra-embryonic endoderm and provide a new cell culture model of an early mammalian lineage.

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“…Visceral endoderm is well-known to be a polarized epithelium [68, 69] that supplies maternal nutrients to early mammalian embryos [70]. During normal development in the mouse (but not in ruminants), IgGs are endocytosed by VE cells [71, 72] and accumulated in vesicles called apical vacuoles (a specialized type of lysosome—[21, 73, 74]).…”

Section: Resultsmentioning

confidence: 99%

“…Cells from primitive streak, identified via BRACHYURY (T) expression [45, 75], were also negative for HOXB9 (Fig 5B2—section a). At this stage, EVE cells are squamous at the distal pole of the embryo, whereas they tend to be more cuboidal and columnar with a more vacuolated cytoplasm when localized close to ExVE [68, 76]. The flattened cells of VE presented both cytoplasmic and nuclear HOXB9 localizations (Fig 5B3—section b—yellow arrows), while the cuboidal (Fig 5B2—section a—yellow arrowheads) and the more columnar (Fig 5B3—section a—yellow asterisk) cells showed HOXB9 localization in apical vacuoles similar to the one observed in E5.5.…”

Section: Resultsmentioning

confidence: 99%

Dynamic Pattern of HOXB9 Protein Localization during Oocyte Maturation and Early Embryonic Development in Mammals

et al. 2016

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BackgroundWe previously showed that the homeodomain transcription factor HOXB9 is expressed in mammalian oocytes and early embryos. However, a systematic and exhaustive study of the localization of the HOXB9 protein, and HOX proteins in general, during mammalian early embryonic development has so far never been performed.ResultsThe distribution of HOXB9 proteins in oocytes and the early embryo was characterized by immunofluorescence from the immature oocyte stage to the peri-gastrulation period in both the mouse and the bovine. HOXB9 was detected at all studied stages with a dynamic expression pattern. Its distribution was well conserved between the two species until the blastocyst stage and was mainly nuclear. From that stage on, trophoblastic cells always showed a strong nuclear staining, while the inner cell mass and the derived cell lines showed important dynamic variations both in staining intensity and in intra-cellular localization. Indeed, HOXB9 appeared to be progressively downregulated in epiblast cells and only reappeared after gastrulation had well progressed. The protein was also detected in the primitive endoderm and its derivatives with a distinctive presence in apical vacuoles of mouse visceral endoderm cells.ConclusionsTogether, these results could suggest the existence of unsuspected functions for HOXB9 during early embryonic development in mammals.

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Ultrastructure of mouse egg-cylinder

Cited by 47 publications

References 5 publications

The hypoblast (visceral endoderm): an evo-devo perspective

The hypoblast (visceral endoderm): an evo-devo perspective

Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts

Dynamic Pattern of HOXB9 Protein Localization during Oocyte Maturation and Early Embryonic Development in Mammals

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