Ultrastructure of Macrophages of the Murine Peyer’s Patch Dome

Hammer, R. F.; Joel, D.D.; LeFevre, M. E.

doi:10.1159/000163175

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…For PLG microspheres Eldridge et al [14,15,21], described the presence of fluorescent parti cles in Peyer's patches for 35 days, results not supported by our work. Our findings rather correlate with those o f Ham mer et al [36] and Jani et al [22] that microspheres are eliminated from Peyer's patches within a few days. As mi crospheres are taken up rapidly and then eliminated within a few days, the most critical parameter is antigen release within this time frame.…”

Section: Discussionsupporting

confidence: 91%

“…bi ological fluids, and thus remains associated to the matrix. In this context, Hammer et al [36] postulated that the major portion o f resorbed particles are transported from the Peyer's patches back into the lumen, and only a small amount of particulate material is translocated into mesen teric lymph nodes and distinct compartments of the system ic immune system. Jani et al [22] described a rapid sizedependent adsorption of microspheres into intestinal Peyer's patches and translocation to other lymphatic com partments within 24 h. In accordance with our findings, they observed a size-dependent decrease in fluorescent beads in Peyer's patches and mesenteric lymph nodes.…”

Section: Discussionmentioning

confidence: 99%

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Oral Immunization with Poly-(D,L-Lactide-Co-Glycolide) and Poly-(L-Lactic Acid) Microspheres Containing Pneumotropic Bacterial Antigens

Kofler¹,

Ruedl²,

Rieser³

et al. 1997

Int Arch Allergy Immunol

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Encouraged by recent findings showing the usefulness of nonreplicating antigen delivery systems in the induction of mucosal immune responses, we investigated microspheres as a means to deliver LW 50020, an immunomodulator consisting of lysates of seven common respiratory pathogens. BALB/c mice were orally immunized with LW 50020 encapsulated into poly-(D,L-lactide-co-glycolide) (PLG) and poly-(L-lactic acid) (PLA) microspheres prepared by either a solvent-evaporation or a solvent-extraction double-emulsion technique. Particle uptake into intestinal Peyer’s patches, induction of antibodies in sera and secretion of immunoglobulins by isolated Peyer’s patches, lungs and spleen lymphocytes were investigated. Our results revealed size and surface characteristic-dependent uptake of microspheres into Peyer’s patches. Microsphere translocation into Peyer’s patches was efficient for 0.8-µm microspheres, but poor for 2.0-µm and surface-modified microspheres. We showed an enhanced immune response in the lungs and sera following oral immunization with 0.8-µm PLG solvent-evaporation microspheres. The immunomodulation was statistically significant as compared to free LW 50020. In contrast, oral immunization with other preparations caused reduced or absent modulation of the immune response compared to 0.8-µm microspheres and free antigen. These findings indicate that microspheres displaying small particle sizes, rapid antigen release and high antigen content provide optimal tools to deliver orally applied antigens.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Oral Immunization with Poly-(D,L-Lactide-Co-Glycolide) and Poly-(L-Lactic Acid) Microspheres Containing Pneumotropic Bacterial Antigens

Kofler¹,

Ruedl²,

Rieser³

et al. 1997

Int Arch Allergy Immunol

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“…The present observations extend previous reports on the heterogeneity of Peyer's patch macrophages [5,10,21,22], It should be stressed that these heterogeneous macro phages are not distributed randomly, but re side in specific locations. We found that mac rophages near the intestinal lumen showed intense acid phosphatase activity and con tained many large lysosome-like bodies whose sudanophilic lipid material resisted extraction by the solvents used in histological processing.…”

Section: Discussionsupporting

confidence: 91%

Particles and Macrophages in Murine Peyer’s Patches

LeFevre¹,

Warren²,

Joel³

1985

Pathobiology

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Mice were given 1 % suspensions of 5 insoluble particles (chrysotile asbestos, quartz, carmine, carbon, and iron oxide) in drinking water for 3 months. The particles were subsequently sought in intestinal Peyer’s patches by light microscopy. Carbon and iron oxide particles were visible in Peyer’s patch macrophages, particularly in the subepithelial region, but the other particles could not be detected. The findings suggest that particle surface properties as well as particle size govern accumulation in Peyer’s patches. The cytochemistry of sub-epithelial, mid-dome, tingible-body, and serosal macrophages of control mice indicated diversity of macrophages within the patch. Macrophages of asbestos-fed mice contained more lysosomes than macrophages of controls. Macrophage abundance in the dome apex was not significantly altered by asbestos ingestion. The other particles did not produce detectable alterations in macrophage morphology.

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“…Considering the weak radiological toxicity of depleted uranium and the low daily quantities of uranium after internal contamination, it seems obvious that possible irradiation effects on the lymphoid follicles present in Peyer's patches were inconceivable for the present study. The question was thus to understand if depleted uranium induced biological modifications of the Peyer's patch function and structure via its chemical toxicity (Leggett, 1989), since a previous study showed modifications of ultrastructural characteristics of macrophages in the Peyer's patch dome with visualization of degenerating macrophages after feeding mice with carbon particles (Hammer et al, 1983).…”

Section: Discussionmentioning

confidence: 99%