Ultrastructure of Intermediate Lobe of the Pituitary and Melanocyte-Stimulating Hormone Secretion in Oestrogen-Induced Kidney Tumours in Male Hamsters

Saluja, Pulin; Hamilton, John M.; Thody, A. J.; Ismail, Abdel Azim A.; Knowles, Jindřiška

doi:10.1007/978-3-642-67265-1_3

Cited by 7 publications

(2 citation statements)

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“…This kind of tumor of the intermediate lobe was graded as benign and classified as melanotroph adenoma . Neoplastic transformation of the intermediate lobe has been previously reported in the Syrian hamster exposed to estrogens (Hamilton et al 1975;Saluja et al 1979) and is accompanied by elevated serum α-MSH concentration (Kurl et al 1990). ER mRNA has been demonstrated by in situ hybridization in the intermediate lobe of rodents (Pelletier et al 1988;Stefaneanu et al 1994) and protracted estrogen treatment can also lead to the development of intermediate lobe tumors in some strains of rats.…”

Section: Discussionmentioning

confidence: 89%

“…In particular, DES treatment causes hyperplasia in the pituitary of male hamster and in the long term the development of an adenoma in the intermediate lobe Saluja et al 1979). In particular, DES treatment causes hyperplasia in the pituitary of male hamster and in the long term the development of an adenoma in the intermediate lobe Saluja et al 1979).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemistry of the golden hamster pituitary during chronic administration of diethylstilbestrol: a quantitative analysis using confocal laser scanning microscopy

Cauwenberge

Nonclercq

Laurent

et al. 2001

Histochem Cell Biol

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The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to diethylstilbestrol (DES). Cell proliferation in the hypophysis was monitored by the immunohistochemical demonstration of S-phase cells after pulse labeling with 5-bromo-2′-deoxyuridine. Cell proliferation analysis was combined with the identification of different cell populations by immunostaining with antisera raised against hypophyseal hormones. Sections processed for double-label immunofluorescence were examined by confocal microscopy. In the adenohypophysis, the relative surface occupied by gonadotrophs and thyrotrophs decreased rapidly during the first months of treatment while corticotroph and somatotroph populations remained unaffected. Accordingly, the incidence of S-phase cells in these four cell populations was lower than or similar to control values. In contrast, lactotrophs increased gradually during the first month of exposure to DES to reach a maximum value at 2-4 months. At the beginning, this increase was primarily due to hyperplasia but later on it also involved cellular hypertrophy. Somatomammotrophs did not seem to be involved in this model. In the pars intermedia, the labeling index of melanotrophs rose rapidly to reach values 5-6 times higher than controls. After 4 months, neoplasms originating from the pars intermedia were seen invading both the neuro-and the adenohypophysis. At the end of treatment, the pituitary was markedly enlarged resulting from the development of an adenoma of the pars intermedia.

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