Ultrastructure of human spleen in transmission and scanning electron microscope

Polák, Štefan; Gálfiová, Paulína; Varga, Ivan

doi:10.2478/s11756-009-0046-2

Cited by 6 publications

(4 citation statements)

References 6 publications

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“…The white pulp of moorhen's spleen appeared to be predominantly formed from lymphocytes of different sizes. Similar finding was reported by Polák, Gálfiová, and Varga (2009) in the human spleen. In addition to that, follicular dendritic cells were noted within the lymphoid follicles by the present study.…”

Section: Discussionsupporting

confidence: 91%

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Abdellatif

2021

Microscopy Res & Technique

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The spleen is the largest secondary lymphoid organ with major roles in the removal of blood-borne antigens. The Eurasian moorhen is a wild aquatic bird that revealed the adaptation to harsh environmental conditions. Information regarding the structural features of moorhen's spleen is lacking. The present study aimed to describe the composition of moorhen's spleen using anatomical dissection, histology, histochemistry, immunohistochemistry, and transmission electron microscopy. The spleen appeared as a brownish red sickle-shaped organ close to the proventriculus and gizzard. The splenic capsule was very thin, and the trabeculae were poorly developed. The white pulp formed of the periarteriolar lymphoid sheath, lymphoid follicles, and periellipsoidal lymphatic sheath. The red pulp composed of sinusoids and cords and contained various types of blood cells. Blood vessels were observed within the splenic capsule and inside the parenchyma. Notably, penicillar capillaries (PCs) appeared branched and surrounded by welldeveloped ellipsoids. Direct connections were observed between PCs and splenic sinusoids suggesting a closed type of circulation. Ultrastructurally, intercellular spaces and vascular channels were evident in the wall of PCs. Ellipsoid-associated cells, lymphocytes, monocytes, and heterophils were observed within splenic ellipsoids. Ellipsoids were thus suggested as a crucial component of moorhen's spleen. Numerous MafB-immunoreactive (IR) macrophages were seen within the red pulp. Splenic cords contained the highest number of PHH3-IR cells, while CCASP3-IR cells were exclusive to follicles of the white pulp. In conclusion, the structure of moorhen's spleen revealed species-specific features.The findings of the present study could help to improve the immunity of domestic birds. Highlights• The Eurasian moorhen is a wild aquatic bird adapted to harsh environmental conditions with no known information about the cellular composition of its spleen.• The Eurasian moorhen's spleen revealed numerous penicillar capillaries surrounded by well-developed ellipsoids and appeared directly connected to the splenic sinusoids forming a closed type of circulation.• Immunohistochemical staining revealed site-specific staining for macrophages, proliferating, and apoptotic cells within moorhen's spleen.

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Section: Discussionsupporting

confidence: 91%

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Abdellatif

2021

Microscopy Res & Technique

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“…Macrophages play an important role in removing dead cells and old red blood cells (Burke & Simon, 1970). Macrophages and dendritic cells were very active in cleaning exogenous materials and bacteria from the body (Polák et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Architecture and Cellular Composition of the Spleen in the Japanese Quail (Coturnix japonica)

Mustafa

El‐Desoky

2020

Microsc Microanal

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The spleen is considered a key player in birds’ immunity. The stroma and the parenchyma of the spleen of the adult quail were demonstrated histologically, histochemically, and ultrastructurally. A thin capsule and the absence of trabeculae were the most characteristics of spleen stroma. The demarcation between white pulp and red pulp was not observed in the quail. White pulp formed from the periarterial lymphatic sheath and the periellipsoidal lymphatic sheath, both of which were surrounded by arteriole and ellipsoid, respectively. Ellipsoids appeared more numerous and were characterized by cuboidal lining of the epithelium and supporting cells. Red pulp consisted of sinuses and cords. White pulp and red pulp of the quail spleen contained various cells, such as red blood cells, macrophages, heterophils with characteristic granules, lymphocytes of different sizes, dendritic cells, plasma cells, and telocytes. In addition, closed circulation and open circulation established the blood flow on the spleen.

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“…Also, the accumulation in the spleen did not change compared to the previous 90 Ylabeled and the 188 Re-labeled antibodies. The hematogenous red pulp of the spleen is known to contain granulocytes, which are the target of anti-CD66-mAb [51][52][53][54][55][56]. The accumulation of the antibody in the bone marrow and spleen depends on the amount of maturing granulopoiesis in these organs and is likely to vary considerably.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Radiolabeling of a [90Y]Y-Anti-CD66-Antibody for Radioimmunotherapy before Allogeneic Hematopoietic Cell Transplantation

Winter,

Hamp-Goldstein,

Fischer

et al. 2023

Cancers

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For patients with acute myeloid leukemia, myelodysplastic syndrome, or acute lymphoblastic leukemia, allogeneic hematopoietic cell transplantation (HCT) is a potentially curative treatment. In addition to standard conditioning regimens for HCT, high-dose radioimmunotherapy (RIT) offers the unique opportunity to selectively deliver a high dose of radiation to the bone marrow while limiting side effects. Modification of a CD66b-specific monoclonal antibody (mAb) with a DTPA-based chelating agent should improve the absorbed dose distribution during therapy. The stability and radioimmunoreactive fraction of the radiolabeled mAbs were determined. Before RIT, all patients underwent dosimetry to determine absorbed doses to bone marrow, kidneys, liver, and spleen. Scans were performed twenty-four hours after therapy for quality control. A radiochemical purity of >95% and acceptable radioimmunoreactivity was achieved. Absorbed organ doses for the liver and kidney were consequently improved compared to reported historical data. All patients tolerated RIT well with no treatment-related acute adverse events. Complete remission could be observed in 4/5 of the patients 3 months after RIT. Two patients developed delayed liver failure unrelated to the radioimmunotherapy. The improved conjugation and radiolabeling procedure resulted in excellent stability, radiochemical purity, and CD66-specific radioimmunoreactivity of 90Y-labeled anti-CD66 mAb. RIT followed by conditioning and HCT was well tolerated. Based on these promising initial data, further prospective studies of [90Y]Y-DTPA-Bn-CHX-A″-anti-CD66-mAb-assisted conditioning in HCT are warranted.

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Ultrastructure of human spleen in transmission and scanning electron microscope

Cited by 6 publications

References 6 publications

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Architecture and Cellular Composition of the Spleen in the Japanese Quail (Coturnix japonica)

Optimization of Radiolabeling of a [90Y]Y-Anti-CD66-Antibody for Radioimmunotherapy before Allogeneic Hematopoietic Cell Transplantation

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