Ultrastructure of BHK-21 cells treated with dopamine

Shubina, V. S.; Abramova, M. B.; Lavrovskaya, V. P.; Павлик, Л. Л.; Lezhnev, E. I.; Мошков, Д. А.

doi:10.1134/s1990519x10010104

Cited by 2 publications

(2 citation statements)

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“…It is known that DA alters the cell ultrastructure and organization of actin cytoskeleton, 29,30 but, to our surprise, the only publication on DA/biomembrane interactions we have found was a molecular dynamics study assisted by a few measurements of surface pressure of lipid monolayer containing DA, a paper published very recently by Orłowski et al 31 Therefore, we decided to carry out a series of experiments in order to obtain thermodynamic description of DA/membrane interactions. According to our knowledge, this is the first experimental study in this field.…”

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confidence: 99%

First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes

Jodko-Piórecka

Litwinienko

2013

ACS Chem. Neurosci.

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Dopamine is essential for receptor-related signal transduction in mammalian central and peripheral nervous systems. Weak interactions between the neurotransmitter and neuronal membranes have been suggested to modulate synaptic transmission; however, binding forces between dopamine and neuronal membranes have not yet been quantitatively described. Herein, for the first time, we have explained the nature of dopamine interactions with model lipid membranes assembled from neutral 1,2-dimyristoyl-snglycero-3-phosphocholine (DMPC), negatively charged 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), and the mixture of these two lipids using isothermal titration calorimetry and differential scanning calorimetry. Dopamine binding to anionic membranes is a thermodynamically favored process with negative enthalpy and positive entropy, quantitatively described by the mole ratio partition coefficient, K. K increases with membrane charge to reach its maximal value, 705.4 ± 60.4 M −1 , for membrane composed from pure DMPG. The contribution of hydrophobic effects to the binding process is expressed by the intrinsic partition coefficient, K 0 . The value of K 0 = 74.7 ± 6.4 M −1 for dopamine/DMPG interactions clearly indicates that hydrophobic effects are 10 times weaker than electrostatic forces in this system. The presence of dopamine decreases the main transition temperature of DMPG, but no similar effect has been observed for DMPC. Basing on these results, we propose a simple electrostatic model of dopamine interactions with anionic membranes with the hydrophobic contribution expressed by K 0 . We suggest that dopamine interacts superficially with phospholipid membranes without penetrating into the bilayer hydrocarbon core. The model is physiologically important, since neuronal membranes contain a large (even 20%) fraction of anionic lipids.

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confidence: 99%

First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes

Jodko-Piórecka

Litwinienko

2013

ACS Chem. Neurosci.

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“…47 Dopamine also alters the cell ultrastructure and organization of the actin cytoskeleton of mammalian cells. 48,49 The precise PDA structure is not established; however, it is often presented as a catechol/ amine/quinone/indole heteropolymer or eumelanine-like oligoindoles. 47 This study confirmed that PDA-Zn@CuO completely inhibited the growth of E. coli as confirmed by an SEM micrograph obtained for this affected bacterium (Figure 5).…”

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confidence: 99%

Antimicrobial Activities of Zn-Doped CuO Microparticles Decorated on Polydopamine against Sensitive and Antibiotic-Resistant Bacteria

Maruthapandi¹,

Saravanan²,

Das³

et al. 2020

ACS Appl. Polym. Mater.

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A polydopamine (PDA) composite was synthesized by depositing Zn-doped CuO (Zn@CuO) particles on polydopamine by one-step ultrasonication. XPS, XRD, and FTIR confirmed the formation of spherical Zn@CuO on PDA with resulting diameters ranging from 1 to 5 μm. The PDA-Zn@CuO at 0.125−0.5 mg/mL exhibited a superior activity over Zn@CuO against sensitive and antibiotic-resistant bacteria. The Zn@CuO nanosized hybrid particles could penetrate the cell but interacted less effectively with bacterial biomolecules to provoke rapid cell lysis. PDA above 0.5 mg/mL also had a modest effect against Escherichia coli and Staphylococcus aureus. However, much higher PDA concentrations were required to restrict the flow of essential nutrients and wastes in and out of the cytoplasmic matrix and resulted in eventual cell death. The binding of PDA-Zn@CuO to bacteria played an important role in causing bacterial death, whereas reactive oxygen species (ROS) also effectively contributed to cell killing.

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Ultrastructure of BHK-21 cells treated with dopamine

Cited by 2 publications

References 11 publications

First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes

First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes

Antimicrobial Activities of Zn-Doped CuO Microparticles Decorated on Polydopamine against Sensitive and Antibiotic-Resistant Bacteria

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