Ultrastructure of articular cartilage of achondroplastic mice

Silberberg, Ruth; Hasler, Mary; Lesker, Peggy A.

doi:10.1159/000144670

Cited by 13 publications

(7 citation statements)

References 2 publications

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“…In some cases among the mitochondria of a usual appearance there appear also small amounts of mitochondria with a strikingly light matrix and slightly damaged cristae. They are probably disintegrating mitochondria as was also stated by Kaplan and Meyer (1959), Meachim et al (1965), Weiss and Mirrow (1972), Ghadially et al (1970), Mitchell and Shepard (1970), Dearden et al (1974), Gritzka et al (1974), Refior (1974), Rother and Redel (1975), Hanaoka (1976), Podrushniak and Cerkasov (1976), Silberger et al (1976), and others especially on the basis of investigations of these organelles in ageing cartilage and initial forms of arthroses. Ri b 0 so m es in the cytoplasm of chondrocytes of the superficial layer are mostly bound to membrane of the granular endoplasmic reticulum.…”

Section: B) Cytoplasmmentioning

confidence: 56%

Submicroscopic Structure of the Human Joint Cartilage

Horký¹

1980

Acta Vet. Brno

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H 0 r k yD.: Submicroscopic Structure of the Human Joint Cartilage. Acta vet. Brno, 49, 1980: 145-176.Using a transmission and scanning electron microscope the joint cartilage was studied in 43 persons of the age from 5 to 75 years. As far as possible the present study gives a finished survey of the ultrastructure of chondrocytes of the superficial, middle and deep layers of the articular cartilage, arrangement of the intercellular substance; presented are basic data on the formation of the surface of the cartilage with regard to the age of the object. Special attention is given to cartilage intercellular substance which -from the point of view of electron microscopy -has been studied only little. The components of intercellular substance are arranged in such a way that we can distinguish the specialized areas near the chondrocytes as the pericellular matrix on the one hand, and the other intercellular substance, the intercellular matrix, on the other. The surface of the cartilage is covered with a specialized layer designated as lamina splendens. Results of the study proper are confronted with published data not only with regard to human joint cartilage but data on these structures have been studied also in lower mammals as much knowledge holds generally true for this type of tissue. The study summarizes the known facts about joint cartilage and provides a necessary basis for judging the degrees of changes occurring during some of their injuries. Ultrastructure, hip joint cartilage, chondrocytes, intercellular matrix, SEM.A number of authors dealt with the description of submicroscopic structure of joint cartilage; principal studies dealing with human cartilage were published by Zelander (1959), Godman et al. (1960), Fawcett (1966a), Meachim (1967), Stockwell (1967a), Silberger (1968, Weiss et al. (1968). Brower and Hsu (1969), Roy (1969), Meachim (1969), Meachim and Roy (1969), Hirohata and Morimoto (1971), Stockwell andMeachim (1973), Serafini-Fracassini and Smith (1974), and others. The present study gives the most possible finished survey about the ultrastructure of both the chondrocytes of the joint cartilage and the intercellular substance; given are also basic data on the formation of its surface in dependence on the age of the object. Results of our study are confronted with published data not only with regard to human joint cartilage but also to data on lower mammals as much knowledge holds generally true for this type of tissue. This fact has been proved in studies published by Barnett et al.

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Section: B) Cytoplasmmentioning

confidence: 56%

Submicroscopic Structure of the Human Joint Cartilage

Horký¹

1980

Acta Vet. Brno

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confidence: 62%

“…Changes in morphology of articular cartilage are associated, to a major extent, with age (except for species-related changes). Such changes have been reported for instance in adult mice (Silberger et al 1976), rabbits (Barnett et al 1963; Davies et al 1962), dogs (Lust et al 1972;Lust and Sherman 1973;Wiltberger and Lust 1975), cattle (Horky 1983(Horky , 1984 and man (Ghadially and Roy 1969;Ghadially 1978Ghadially , 1982Horky 1980) and to some extent in pigs (Grondalen 1974c, f;Nakano et al 1979a, b). However, only the work by Bhatnagar et al (1981) has been concerned with the ultrastructure of porcine articular cartilage in the postnatal period.…”

Section: Ultrastructure Porcine Articular Cartilage Developmentmentioning

confidence: 85%

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Horký¹

1989

Acta Vet. Brno

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Horky D.: The Ultrastructure of Articular Cartilage in the PrenatalPig. Acta vet. Brno, 58,1989: 143-174.The articular cartilage of the femoral articulation was studied in 14 porcine foetuses of both &exes at 41, 61 and 94 days after fertilization. The structure of articular chondroblasts and intercellular matter enabled us to distinguish three zones as early as at 41 days. The surface zone contained cells at a low degree of differentiation in structure resembling fibroblasts. The medium zone showed thick bundles of collagen microfibrils penetrating through the cell membrane into the intercellular matter. At 61 days both the chondroblast cytoplasm and the cartilage surface of the medium zone had vesicles, several !-10m in size, bounded by a single membrane. At 91 days after fertilization the cytoplasm of chondroblasts found at the border between the surface and the medium zones revealed collagen microfibrils running through cell memblanes. The fibrils showed signs of periodicity.The surface layer of the cartilage in the period under study was made up largerly of aperiodic fibrils. In some regions below the surface bundles of collagen microfibrils ran parallel to the surface of the articular cartilage. They later became involved into the chondrosynovial membrane. Ultrastructure, porcine articular cartilage, developmentIn the course of articular cartilage development both chondrocytes and intercellular matter are subject to differentiation processes which, in the prenatal period, lead to the formation of articular cartilage followed by further changes in the two components in the neonatal period. The changes in the developing articular cartilage are referred to as the maturation process and are determined genetically, endocrinologically, by nutritional conditions (Silberger and Silberger 1941;Silberger et at. 1961) and, particularly in the postnatal period, by endogenous environmental factors (Ghadially 1978(Ghadially , 1981(Ghadially , 1982Perrin et al. 1978). Changes in morphology of articular cartilage are associated, to a major extent, with age (except for species-related changes). Such changes have been reported for instance in adult mice (Silberger et al. 1976), rabbits (Barnett et al. 1963; Davies et al.

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“…It functions as a lubricated surface for articulation by reducing friction and facilitating the transmission of loads. Study of cartilage-related diseases showed that the abnormal changes in morphology and function of cartilage are associated with various pathologies, such as osteoarthritis, achondroplasia and costochondritis [1][2][3][4][5][6]. Chondrocytes, the only resident cells in healthy cartilage, are responsible for the production and maintenance of the cartilaginous tissue.…”

Section: Introductionmentioning

confidence: 99%

Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development

Xue

Duan

et al. 2017

Biomed. Opt. Express

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Abstract:We developed a multimodal nonlinear optical (NLO) microscope system by integrating stimulated Raman scattering (SRS), second harmonic generation (SHG) and twophoton excited fluorescence (TPEF) imaging. The system was used to study the morphological and biochemical characteristics of tibial cartilage in a kinesin-1 (Kif5b) knockout mouse model. The detailed structure of fibrillar collagen in the extracellular matrix of cartilage was visualized by the forward and backward SHG signals, while high resolution imaging of chondrocytes was achieved by capturing endogenous TPEF and SRS signals of the cells. The results demonstrate that collagen fibrils in the superficial surface of the articular cartilage decreased significantly in the absence of Kif5b. The distorted morphology along with accumulated intracellular collagen was observed in the Kif5b-deficient chondrocytes, indicating the critical roles of kinesin-1 in the chondrocyte morphogenesis and collagen secretion. The study shows that multimodal NLO imaging method is an effective approach to investigate early development of cartilage.

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Ultrastructure of articular cartilage of achondroplastic mice

Cited by 13 publications

References 2 publications

Submicroscopic Structure of the Human Joint Cartilage

Submicroscopic Structure of the Human Joint Cartilage

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Multimodal nonlinear optical microscopy reveals critical role of kinesin-1 in cartilage development

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