Ultrastructure as a basis for dating of rat endometrium

Spornitz, Udo M.; Rinderknecht, B. P.; Edelmann, A.; Scheidegger, B.; Cairoli, F.

doi:10.1002/ar.1092380203

Cited by 43 publications

(54 citation statements)

References 22 publications

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“…The present study shows that extra lipid accumulation occurs within the uterine epithelium suggesting that hypothyroidism may influence generally cellular lipid metabolism. The fact that the observed changes in epithelial structure in this study were similar to those found during dioestrus stage of the oestrus cycle in rats by Spornitz et al (36) further suggest that hypothyroidism affects ovarian steroid hormone metabolism. Therefore, alteration in steroid hormone levels during hypothyroidism is especially likely since the dioestrus stage in rats is characterised by low steroid hormone levels, and the hypothyroid rats in this study had a preponderance of dioestrus vaginal smears (12).…”

Section: Discussionsupporting

confidence: 90%

A Morphometric Study on the Endometrium of Rat Uterus in Hypothyroid and Thyroxine Treated Hypothyroid Rats

Inuwa¹,

Williams²

2006

Upsala Journal of Medical Sciences

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Hypothyroidism increases the rate of pregnancy loss. Other manifestations include menstrual disorder, and infertility. Serum levels of gonadotropins are low in hypothyroid patients. Though studies of uterine ultrastructure are well established as approaches to investigating the pathophysiology of infertility, they have scarcely been extended to the study of hypothyroid related infertility. The present study investigates the effect of hypothyroidism on the ultrastructure of uterine epithelium.Three groups of Wistar rats were studied. Two groups were initially made hypothyroid using methimazole, and the third group was an untreated control. One hypothyroid group was given daily injections of thyroxine for six weeks. The uteri were removed in all three groups, and processed for transmission electron microscopy and morphometry. It was found that absolute epithelial cell volume was decreased in hypothyroidism. The volume of the nucleus had decreased though its relative volume in the cell had increased. The height of the luminal epithelium in hypothyroid rats also decreased by (33.8%) as compared with controls. Basement membrane thickness was significantly increased in hypothyroidism. The changes were all substantially abrogated by the administration of thyroxine.This study suggests that thyroid hormones might be importantly concerned in the maintenance of the normal structure of uterine epithelial cells.

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Section: Discussionsupporting

confidence: 90%

A Morphometric Study on the Endometrium of Rat Uterus in Hypothyroid and Thyroxine Treated Hypothyroid Rats

Inuwa¹,

Williams²

2006

Upsala Journal of Medical Sciences

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“…For this purpose endometrial tissue, predated by vaginal smear cytology, was verified by hormone assessment and then investigated by scanning electron microscopy. This study complements the work done by Spornitz et al (1994), who examined intracellular changes in epithelial cells under the same conditions.…”

supporting

confidence: 73%

Estrous stage determination in rats by means of scanning electron microscopic images of uterine surface epithelium

1999

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With the results presented in this paper we devised an alternative method to precisely date the rat endometrium in relation to the estrous cycle. This is done by the exclusive use of scanning electron microscopy.Owing to its short estrous cycle (4 to 6 days, depending on age), the rat is ideally suited for the examination of cyclic changes occurring in the uterine epithelium. The cycle stage of rats predated by vaginal smear cytology was verified through the measurement of hormones relevant to the estrous cycle, i.e. estradiol-17-beta (E2), progesterone (P), luteinizing hormone (LH), and follicle stimulating hormone (FSH). Based on scanning electron images surface changes could thus be correlated to the cyclic variations of blood levels of sex hormones. The appearance of pseudoglands, the most prominent aspect during the cycle, is correlated with decreasing estrogen and rising progesterone levels. Pseudoglands are formed by apoptosis and necrosis of epithelial cells, and are most numerous during estrus. They had in previous studies been classified as genuine uterine glands. Anat Rec 254: 116-126, 1999.

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“…While oestrogen affects the endometrium during the proliferative phase, progesterone induces several morphological and functional changes in the endometrium during the secretory phase of the cycle. The secretory changes in the endometrial epithelial and stromal compartments are well documented (Noyes et al, 1950;Li et al, 1991;Spornitz et al, 1994). Morphological features in the control endometrium similar to those reported by above investigators were also observed in the present study.…”

Section: Resultssupporting

confidence: 91%

“…Several studies indicated an increased secretory function, and a change in endometrial glandular epithelial structure in humans, rats and sheep (Smith et aL, 1990; Li et al, 1991; Blankenship and Given, 1992; Spornitz et al, 1994). Along with this secretory activity, epithelial cells increase their number of nuclei, then differentiate into a special type of cell, the so-called Arias-Stella cells.…”

Section: Resultsmentioning

confidence: 99%

Blastocyst Implantation in Rats: A Morphological Approach

Gürsoy

Eray

Yücel

1998

Okajimas Folia Anatomica Japonica

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Summary: Very complex cellular events take place at the morphological and molecular levels both in the maternal and embryonic sites during blastocyst implantation. Although the morphological alterations in the gestational stages of the implantation site are well-known, mechanisms of the interaction between blastocyst and endometrial epithelium (apposition and adhesion stages) and penetration of the blastocyst into the endometrium have not yet been fully described. Therefore the present study was designed to determine these events morphologically throughout the implantational stages.To do this the endometrial tissues containing implantation sites from pregnant rats were taken 5 and 8 days after fertilization. In addition, non-pregnant rat endometrial tissues were also obtained in order to use as control. Tissues were processed for light and electron microscopy. Semi-thin and ultra-thin sections were stained using appropriate staining methods, and were evaluated under light and electron microscope.In the present study, interaction between trophoblast and luminal epithelium, penetration of the blastocyst into the endometrium, differentiation of germinal layers in the blastocyst and tissue alterations such as decidualization in the maternal site have been demonstrated morphologically.In condusion, while blastocyst was implanted into the endometrium, distinct morphological changes occurred both in maternal and embryonic sites. The following stages of implantation were the development of germinal layers in the embryo and morphological changes in the maternal site leading to the placentation.

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Ultrastructure as a basis for dating of rat endometrium

Cited by 43 publications

References 22 publications

A Morphometric Study on the Endometrium of Rat Uterus in Hypothyroid and Thyroxine Treated Hypothyroid Rats

A Morphometric Study on the Endometrium of Rat Uterus in Hypothyroid and Thyroxine Treated Hypothyroid Rats

Estrous stage determination in rats by means of scanning electron microscopic images of uterine surface epithelium

Blastocyst Implantation in Rats: A Morphological Approach

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