Ultrastructure and Mitochondrial Vesiculation Associated with Closteroviruslike Particles in Leafroll-Diseased Grapevines

Kim, K. S.; Téliz, D.; Lee, K. W.

doi:10.1094/phyto-79-357

Cited by 19 publications

(12 citation statements)

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“…Cytopathological modifications, which are prominent in differentiating sieve tubes, companion cells and phloem parenchyma cells, are characterized by the presence of: (i) inclusion bodies made up of membranous vesicles 50–100 nm in diameter, derived from proliferation of the bounding membrane of mitochondria (Kim et al, 1989). These vesicles, which are released in the cytoplasm following disruption of mitochondria (Faoro et al, 1992), contain a network of fine fibrils identified as RNA, and are thought to be sites of replication (Faoro and Carzaniga, 1995); (ii) loose bundles to compact aggregates of virus particles that often fill the lumen of sieve tubes and may also be localized in the nuclei.…”

Section: Host-pathogen Interactionsmentioning

confidence: 99%

Grapevine leafroll-associated virus 3

Maree¹,

Almeida²,

Bester³

et al. 2013

Front. Microbiol.

197

186

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Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a general overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classification of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional serological assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics. The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management.

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Section: Host-pathogen Interactionsmentioning

confidence: 99%

Grapevine leafroll-associated virus 3

Maree¹,

Almeida²,

Bester³

et al. 2013

Front. Microbiol.

197

186

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“…The NY1 isolate of GLRaV-3 used in this study was first characterized in our laboratory by Zee et al (1987). We demonstrated that closterovirus-like particles were closely associated with the disease and BYV-type vesicles were observed in leafroll-infected tissues (Zee et al, 1987 ;Kim et al, 1989). GLRaV-3-specific antiserum and monoclonal antibodies were produced against purified virions (Zee et al, 1987 ;.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus.

Ling¹,

Zhu²,

Drong³

et al. 1998

Journal of General Virology

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“…A feature of infection by some closteroviruses is the production of many small membrane-bound vesicles, possibly containing dsRNA, which are often found in infected cells (Esau & Hoefert, 1971). Such structures, first found associated with beet yellows virus (BYV) infections, have been termed BYV-type vesicles, and after much speculation as to their origin the membranes have recently been suggested to be derived from the mitochondria, at least for infections with grapevine leafroll virus (Kim et al, 1989). This and other aspects of the cytopathology associated with closterovirus infection have been reviewed elsewhere (Lesemann, 1988).…”

Section: Introductionmentioning

confidence: 99%