Ultrastructure and Description of a Fungus‐Feeding Amoeba, Trichamoeba mycophaga n. sp. (Amoebidae, Amoebea), from Australia¹

Abstract

Nematol.. 7 : 34-40. 28. Wallich. G . C. 1863. O n an undescribcd indigenous form of Amoeba. Ann. Mag. Nut. Hist.. 11: 365-371. Received I5 I V 86; accepted 5 VIII 86 t Figs. 14-16. Electron micrographs of Trichamoeh mycophaga. 14. Nucleus showing the single central nucleolus within difise nucleoplasm surrounded by a more dense matrix. Note the peripheral strands of microfibrillar material (arrows). Bar = I pm. 15. Tangential section of nucleus showing nuclear pores (arrows) and microfibrillar material. Bar = 0.5 pm. 16. A section of Fig. I5 detailing microfibrillar material. Bar = 0.1 pm.

Phylogenetic position of the amoeba genus Deuteramoeba (Amoebozoa, Tubulinea)

“…Light-microscopic observations show cells, fitting the previously published descriptions of this species (Old et al, 1985;Chakraborty and Old, 1986;Page, 1988Page, , 1991Mrva, 2010/11). Amoebae were polytactic, with relatively short and non-numerous pseudopodia (Fig.…”

“…The depositor of this strain, according to CCAP record, was Old in the year 1984. In the formal description of this species, Chakraborty and Old (1986) noted that the studied culture was deposited with CCAP (without indication of the exact time). Neither Old et al (1985) nor Chakraborty and Old (1986) who studied the ultrastructure of this amoeba species, found anything resembling these structures, neither in light microscopy nor in the electron-microscopic images of the nucleus.…”

“…In the formal description of this species, Chakraborty and Old (1986) noted that the studied culture was deposited with CCAP (without indication of the exact time). Neither Old et al (1985) nor Chakraborty and Old (1986) who studied the ultrastructure of this amoeba species, found anything resembling these structures, neither in light microscopy nor in the electron-microscopic images of the nucleus. This suggests that either the culture probably got infected during its maintenance in CCAP but the putative infection was not recognized since this group of organisms was not known at that time, or a different strain rather than the one studied by Chakraborty and Old (1986) was deposited.…”

“…Sequences were aligned manually using SeaView 4.0 (Gouy et al, 2010). The phylogenetic analysis was performed using maximum likelihood method as implemented in PhyML program (Guindon and Gascuel, 2003) with GTR + γ + I model suggested by the program Modeltest (Posada and Crandall, 1998); 1471 sites were selected for the analysis.…”

“…The second species of this genus was first described as Trichamoeba mycophaga (Pussard et al, 1980) and transferred to the genus Deuteramoeba by Page (1988) because of its predominantly polypodial locomotive form. This species was isolated from soil (Pussard et al, 1980;Chakraborty and Old, 1986;Mrva, 2005Mrva, , 2010Page, 1988Page, , 1991 and is cyst-forming; this maybe a specific adaptation to its soil lifestyle (Smirnov and Brown, 2004).…”