Ultrastructure alterations of adult male Schistosoma mansoni harbored in albino mice treated with Sidr honey and/or Nigella sativa oil

Abstract

In this investigation, the effects of Sidr honey, Nigella sativa oil (black-seed oil) and Sidr honey and black-seed oil together on the ultrastructure of tegument, gastrodermis and testes of adult male Schistosoma mansoni harbored in albino mice were studied. The tegument of adult male S. mansoni recovered from mice treated with various types of treatments revealed apparent damages which were severely clear in male worms harbored in mice treated with Sidr honey and black-seed oil together. On the other hand, the gastrodermis and testes of adult male S. mansoni obtained from mice treated with Sidr honey only or black-seed only revealed no apparent damage, while the gastrodermis and testes of worms recovered from mice treated with Sidr honey and black-seed oil together showed severe disorganization. Therefore, these treatments may represent a promising alternative treatment for the control of schistosomiasis mansoni, especially in endemic areas and where drug-resistant strains are found. These natural products may be recommended as useful, pleasant and popular accepted elements of food and drinks in such cases.

Transmission electron microscopic observations on ultrastructural alterations in Schistosoma mansoni adult worms recovered from C57BL/6 mice treated with radiation-attenuated vaccine and/or praziquantel in addition to passive immunization with normal and vaccinated rabbit sera against infection

“…Moreover, large accumulation of lipid granules between tegumental cells was recorded. These alterations were compared in accordance with results reported by Mostafa and Soliman (2010) and Xiao et al (2010).…”

“…The number of ova per gram tissue is also an important criterion for the evaluation of the effect of anti-schistosomal agent on the infection (Mostafa 2005). Furthermore, the reduction of the eggs' load in the hepatic tissue in treated mice may be attributed to the reduction in the worm burden as a result of treatment, the low productivity of the female already present, and the active destruction of the few eggs produced by the host's tissue reaction (Mostafa 2001;Mostafa et al 2011).…”

A Novel Treatment of Schistosomiasis: Nano-Calcium Silicate Incorporating 5% Copper Oxide

“…These findings were in agreement with the findings of Staudt et al 37 This may be elucidated by the point that most of the female's body is walled in the gynaecophoric duct of the male [i.e., not in direct contact with the host's microenvironment]. 38 The alteration in the surface architecture of S. mansoni and S. haematobium worms as a result of treatment with CS-5%CuO were more or less like the tegmental alterations in Schistosoma mekongi worms sheltered in mice treated with artesunate (C 19 H 28 O 8 ) as observed by Jiraungkoorskul et al 39 or that studied by Staudt et al on the effects of the enantiomers of PZQ and its main metabolite on in vitro S. mansoni. 37 The current results revealed that the morphological alterations could be a mechanism for killing the worms using CS-CuO.…”

A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models

“…In our study, the abnormal morphological changes were first observed in the tegument of male worms, which is attributed to the more pronounced soft tissue alterations in males than in females (63) . Therefore, the tegumental changes were apparently more prominent in male worms.…”