Ultrastructural Visualization of Surface Carbohydrate Structures on Mycoplasma Membranes by Concanavalin A

Schiefer, Hans-Gerd; Krauss, H; Brünner, Helmut; Gerhardt, Ursula

doi:10.1128/jb.124.3.1598-1600.1975

Cited by 38 publications

(17 citation statements)

References 17 publications

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“…As a model system to perform sensitive measurements of protein binding with different concentrations of protein, we used the specific binding of the glycoprotein concanavalin A (Con A) to ribonuclease B (RNase B) immobilized on a monolayer (11‐mercaptoundecanoic acid), as shown in Figure a. Con A, an important member of the legume lectin family, is widely used in biology and biochemistry to characterize glycoproteins and other sugar‐containing entities on the surfaces of various cells . The specific binding of Con A and RNase B has become a standard affinity model for assessing biosensor performance.…”

Section: Resultsmentioning

confidence: 99%

Large‐Scale Plasmonic Nanodisk Structures for a High Sensitivity Biosensing Platform Fabricated by Transfer Nanoprinting

Liang

Cui

et al. 2019

Advanced Optical Materials

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safety, and environmental monitoring, owing to their stronger near-field confined capacity on the surface, as compared with that of prism-based metallic film sensors. [1][2][3][4][5][6][7][8][9][10][11] Moreover, surface plasmon resonance (SPR) in plasmonic nanostructures can be directly excited by using simple free-space incident light instead of complex and bulky optical prism excitation systems, which exhibits great potential for developing ultracompact and multiplexed sensing applications. [12][13][14][15] Many efforts have been focused on engineering the topography and materials of nanostructures to improve their sensitivity and to realize portable sensing applications, such as nanoholes, [16][17][18] nanodisks, [19][20][21] nanorings, [22,23] and nanomushroom arrays. [24] To date, most plasmonic nanostructure sensors have been fabricated by lithography-based top-down nanofabrication technologies, for example, focused ion beam milling [25,26] and electron beam lithography. [19][20][21][22][23][24] Although they have high preparation precision, good stability, and repeatability, these top-down nanofabrication technologies suffer from some inherent shortcomings, such as high equipment cost, time-consuming fabrication, low yields, and small footprint sizes (usually limited to below 100 µm × 100 µm), which severely restrict the practical applications of plasmonic nanostructure sensors. In addition, because of the abovementioned small sensor footprint, the angle-dependent sensing properties are relatively less studied. [27][28][29] To overcome these drawbacks, bottom-up nanofabrication technologies, such as nanosphere lithography and tunable holographic lithography, are used to generate large-scale ordered nanostructure arrays with low cost and facile fabrication. [30,31] However, large-scale fabrication approaches are mainly used to fabricate sunken nanostructures, such as nanoholes or nanogrooves. [28,31,32] The relatively low sensing capabilities of sunken nanostructures make them uncompetitive with those of prism-based SPR sensors. Therefore, there is an urgent demand for the realization of large-scale, low cost biosensors with convex nanostructures, such as large scale fabrication of nanostructures by laserinduced dewetting and laser ablation methods. [33,34] In this paper, we reported a centimeter-scale, convex plasmonic nanostructure as a sensing platform fabricated by low cost transfer nanoprinting and based on an ultrathin anodic Surface plasmon resonance in plasmonic nanostructures has become a powerful analytical tool in ultrasensitive label-free biomolecule sensing. However, the fabrication of plasmonic nanostructure sensors relies on lithography-based top-down nanofabrication approaches, which have inherent shortcomings, such as high manufacturing cost, time-consuming fabrication, and a small fabrication footprint. In particular, the small footprint of fabricated plasmonic nanostructures has significantly restrained the study of their angle-dependent sensitivity. Here, a centimeter-scale, high sensit...

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Section: Resultsmentioning

confidence: 99%

Large‐Scale Plasmonic Nanodisk Structures for a High Sensitivity Biosensing Platform Fabricated by Transfer Nanoprinting

Liang

Cui

et al. 2019

Advanced Optical Materials

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“…1A). Therefore, most experiments were performed with mycoplasmas prefixed with glutaraldehyde before harvesting and cytochemical labeling in order to stabilize the membranes' gross structure and to prevent redistribution and cluster formation of the ferritin granules (14,29).…”

Section: Resultsmentioning

confidence: 99%

“…In pursuing our previous studies (27)(28)(29) on the surface architecture of mycoplasma membranes, we investigated with the electron microscope the localization, distribution, and chemical nature of the anionic sites on the surfaces of intact mycoplasmas and isolated mycoplasma membranes. As a visual probe, we used a polycationized derivative of ferritin (2) which binds electrostatically to anionic sites on the surface of either glutaraldehyde-fixed or unfixed membranes.…”

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confidence: 99%

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Ultrastructural visualization of anionic sites on mycoplasma membranes by polycationic ferritin

Schiefer¹,

Krauss²,

Brünner³

et al. 1976

J Bacteriol

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Anionic sites on mycoplasma membranes were visualized in the electron microscope by a polycationized ferritin derivative. The technique of thin sectioning was used. Staining prior to fixation led to clustering of ferritin granules on the mycoplasma cell surface. On glutaraldehyde-fixed Mycoplasma mycoides subsp. capri, M. gallisepticum, M. pneumoniae, and Acholeplasma laidlawii, the anionic sites were uniformly distributed over the entire membrane surface. M. hominis did not bind the polycationic ferritin label. Chemical and enzymatic treatments of the mycoplasmas indicated that the anionic sites may be lipid phosphate groups. Isolated M. mycoides subsp. capri membranes were labeled exclusively on only one membrane surface, presumably the outer one. Liposomes prepared from diphosphatidylglycerol and phosphatidylcholine were also labeled by the polycationic ferritin.

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“…Schiefer et al (13) have demonstrated surface carbohydrates ofMycoplasma mycoides var. capri by using the cytochemical staining procedure with ConA.…”

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confidence: 99%

Ultrastructural localization of Thermoplasma acidophilum surface carbohydrate by using concanavalin A

Mayberry-Carson¹,

Jewell²,

Smith³

1978

J Bacteriol

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Ultrastructural Visualization of Surface Carbohydrate Structures on Mycoplasma Membranes by Concanavalin A

Abstract: Surface carbohydrates of Mycoplasma mycoides var. capri were made visible by the cytochemical staining procedure with concanavalin A, horseradish peroxidase, and diaminobenzidine.

Cited by 38 publications

References 17 publications

Large‐Scale Plasmonic Nanodisk Structures for a High Sensitivity Biosensing Platform Fabricated by Transfer Nanoprinting

Large‐Scale Plasmonic Nanodisk Structures for a High Sensitivity Biosensing Platform Fabricated by Transfer Nanoprinting

Ultrastructural visualization of anionic sites on mycoplasma membranes by polycationic ferritin

Ultrastructural localization of Thermoplasma acidophilum surface carbohydrate by using concanavalin A

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