Ultrastructural Variations in Lethocerus niloticum (Insecta: Hemiptera) Caused by Pollution in Lake Mariut, Alexandria, Egypt

Sorour, Jehan

doi:10.1006/eesa.2000.2003

Cited by 31 publications

(22 citation statements)

References 35 publications

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“…The intestine can reveal morphological alterations induced by environmental contaminants absorbed by the insect (Thiboldeaux et al 1998;Sorour 2001).…”

Section: Introductionmentioning

confidence: 99%

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

et al. 2009

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Morphological alterations, by means of histological and ultrastructural analysis, have been used to determine the effects of boric acid and fipronil on midgut tissues of honeybee worker, Apis mellifera L. larvae. In order to observe possible morphological alterations in the midgut, two groups of bioassays were performed. In the first one, the larvae were chronically treated with different concentrations of boric acid added to the food (1.0, 2.5 and 7.5 mg/g). In the second group, the larvae were fed with diets containing different concentrations of fipronil (0.1 and 1 microg/g) and compared with control groups without these chemical compounds. In the first bioassay, the larvae were collected on day 3 and in the second bioassay on day 4, when the mortality rate obtained in the toxicological bioassay was not very high. The larval midguts were removed and processed for morphological analyses using a light and transmission electron microscopy. We observed cytoplasmic vacuolizations, with the absence of autophagic vacuoles, and chromatinic compacting in most of the cells in the groups treated with pesticides. The morphological alterations were far greater in the larvae treated with boric acid than in the larvae treated with fipronil. Our data suggest that the midgut cell death observed was in response to boric acid and fipronil action. This study significantly improves the understanding of the toxicological effect of these insecticides from the ecotoxicological perspective.

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“…The intestine can reveal morphological alterations induced by environmental contaminants absorbed by the insect (Thiboldeaux et al 1998;Sorour 2001).…”

Section: Introductionmentioning

confidence: 99%

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

et al. 2009

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“…The main target organ of boric acid in insects is the gut (Klotz et al 2000), because this compound is usually absorbed orally. The midgut and Malpighian tubules are organs associated with absorption and excretion, respectively, of chemical compounds and an examination of their morphology can reveal ultrastructural changes induced by toxicants , Sorour 2001, Malaspina and Silva-Zacarin 2006. Among these morphological changes are those leading to programmed cell death (by autophagy or apoptosis) or necrosis (Lockshin and Zakeri 2004).…”

mentioning

confidence: 99%

Toxicological and Histopathological Effects of Boric Acid on <I>Atta sexdens rubropilosa</I> (Hymenoptera: Formicidae) Workers

Sumida

Silva-Zacarín

Décio

et al. 2010

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The current study compared the toxicity of different concentrations of boric acid in adult workers of Atta sexdens rubropilosa Forel (Hymenoptera: Formicidae), with toxicological bioassays, and examining the dose-dependent and time-dependent histopathological changes, of the midgut, Malpighian tubules, and postpharyngeal glands. Our results revealed the importance of conducting toxicological bioassays combined with morphological analyses of the organs of ants chronically exposed to insecticides used in commercial ant baits. In vitro bioassays showed that boric acid significantly decreases the survivorship of workers regardless of concentration, whereas the morphological data suggested progressive dose-dependent and time-dependent changes in the organs examined, which were evident in the midgut. The midgut is the first organ to be affected, followed by the postpharyngeal gland and Malpighian tubules. This sequence is in agreement with the absorption pathway of this chemical compound in the midgut, its transference to the hemolymph, possibly reaching the postpharyngeal glands, and excretion by the Malpighian tubules. These progressive changes might be due to the cumulative and delayed effect of boric acid. Our findings provide important information for the understanding of the action of boric acid in ant baits in direct and indirect target organs.

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“…68 The MTs of insects are also known to have mechanisms of active transport to remove toxic compounds such as alkaloids from vegetal sources 8 and chemical compounds that are present in the environment and are toxic for insects. 69 In addition to the obvious importance of the excretory system, the MTs have been widely used in ecotoxicological studies, as they quickly respond to changes in the insect's environment, and the tubules also induce detoxification genes, notably cytochrome P450s and glutathione S-transferases. 70 This highlights the importance of understanding their structure and physiology.…”

Section: Other Functionsmentioning

confidence: 99%

“…70 This highlights the importance of understanding their structure and physiology. 69,[71][72][73] Different functions of MTs have emerged with the development of postgenomic technologies, mainly in Drosophila, such as microarrays, proteomics, and metabolomics. 74 By means of molecular techniques, Chahine and O'Donnell 75 suggest a coordinated response by the MTs, involving both alterations in detoxification pathways as well as enhanced fluid transport.…”

Section: Other Functionsmentioning

confidence: 99%

Comparative physiology of Malpighian tubules: form and function

Cintra-Socolowski¹,

Nocelli²,

Roat³

et al. 2016

OAIP

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Ultrastructural Variations in Lethocerus niloticum (Insecta: Hemiptera) Caused by Pollution in Lake Mariut, Alexandria, Egypt

Cited by 31 publications

References 35 publications

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

Toxicological and Histopathological Effects of Boric Acid on <I>Atta sexdens rubropilosa</I> (Hymenoptera: Formicidae) Workers

Comparative physiology of Malpighian tubules: form and function

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