Ultrastructural Study on the Release Reaction of the Platelet

Kawaguchi, Yukihiro

doi:10.1111/j.1440-1827.1982.tb02078.x

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Platelet shape change is characterized by the breakdown and reorganization of cytoskeletal components (microtubules and microfilaments) which lead to the release of platelet granules [15][16][17]33]. thrombin).…”

Section: In Vitro Studiesmentioning

confidence: 99%

Tumor cell-platelet interactions in vitro and their relationship to in vivo arrest of hematogenously circulating tumor cells

et al. 1987

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Aggregation of rat platelets was induced in vitro by homologous rat Walker 256 carcinosarcoma cells and the extent of tumor cell-platelet interactions examined ultrastructurally. By 30s there was surface contact between unstimulated platelets and tumor cell microvilli. By midphase aggregation (2-3 min) tumor cells became enmeshed within expanding platelet aggregates. Tumor cell microvilli and platelet pseudopodia interdigitated as aggregation progressed. During the later stages of aggregation (6-10 min) tumor cells formed large processes which penetrated deep into the aggregate. Platelet activation (i.e. degranulation) occurred in gradient fashion and was concentrated near tumor cell membrane sites involved in process formation. At these later stages tumor cells near the aggregate periphery were found to have engulfed platelets or platelet fragments. Tumor cell-platelet interactions in the pulmonary microvasculature were also studied in vivo following injection of murine Lewis lung carcinoma, 16C mammary adenocarcinoma, and B16 amelanotic melanoma tumor cells into the tail vein. Platelets demonstrated a biphasic association with arrested tumor cells with peak interactions occurring at 10-30 min and 4-24 h. Ultrastructurally, tumor cells exhibited newly formed processes which interdigitated with the platelet aggregate. Such processes formed only in areas of contact with platelets and not in areas of contact with endothelial cells or other blood elements (i.e. erythrocytes, polymorphonuclear leukocytes). Numerous tumor cell mitochondria were concentrated in the areas of greatest platelet-tumor cell process activity. At early time intervals (2-10 min), intravascular platelet degranulation was observed primarily in platelets associated with tumor cell processes. Tumor cells also were found to have engulfed platelet fragments in vivo.

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Section: In Vitro Studiesmentioning

confidence: 99%

Tumor cell-platelet interactions in vitro and their relationship to in vivo arrest of hematogenously circulating tumor cells

et al. 1987

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“…A few seconds after the addition of ADP, collagen or thrombin a slight decrease in percent light transmission (%T) is observed, followed by a rapid increase in % T which attains its maximum at the generation of secondary aggregates. The morphological alterations of platelets in the aggregometer have been studied extensively by light and transmission electron microscopy (TEM) (2,4,17). SEM studies using the aggregometer method, however, were not available.…”

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confidence: 99%

Surface morphology of human platelets during in vitro aggregation

Zilla

Groscurth

Rhyner

et al. 1984

Scandinavian Journal of Haematology

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The alterations of surface morphology of human platelets during the course of the aggregometer tracing were studied by scanning electron microscopy (SEM). Prior to activation the platelet rich plasma was pre-incubated at 37°C for at least 30 rnin in order to obtain a sufficient number of discoid platelets. Immediately following the addition of collagen or ADP platelets showed slender pseudopods which thereafter were replaced by bulbous protrusions. The decrease in percent light transmission

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Ultrastructural Study on the Release Reaction of the Platelet

Kawaguchi

1982

Acta Pathologica Japonica

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The ultrastructural changes of the platelets stimulated by release inducers II such as thrombin, collagen, and latex particles were studied to compare the differences in morphology of release I and II. The characteristics of platelets in response to thrombin were the decrease in number of alpha‐granules and marked dilation of the open canalicular system containing fibrin preceded by the disappearance of very dense granules. In the platelets in response to collagen or latex, the release of alpha‐granules was initiated after taking the particles into the open canalicular system. In all of the cases, the amount of microfibrils was seen in the cytoplasm, while contraction wave of the microtubules was rare in the cytoplasm during the release and uptake of particles. Accordingly, the release of very dense granules seems to be followed by that of alpha‐granules. It is suggested that the microfibrils play a more important role than the microtubules in the release II as well as release I. Furthermore, the contents of alpha‐granules may be discharged via the open canalicular system on the release II by thrombin, collagen or latex particles.

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Ultrastructural Study on the Release Reaction of the Platelet

Cited by 5 publications

References 28 publications

Tumor cell-platelet interactions in vitro and their relationship to in vivo arrest of hematogenously circulating tumor cells

Tumor cell-platelet interactions in vitro and their relationship to in vivo arrest of hematogenously circulating tumor cells

Surface morphology of human platelets during in vitro aggregation

Ultrastructural Study on the Release Reaction of the Platelet

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