Ultrastructural study on the fertilisation process in sturgeon (Acipenser), function of acrosome and prevention of polyspermy

Pšenička, Martin; Rodina, Marek; Linhart, Otomar

doi:10.1016/j.anireprosci.2009.03.013

Cited by 49 publications

(58 citation statements)

References 21 publications

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“…It is also present in other groups of fishes, such as Perciformes, Cypriniformes, and Cyprinodontiformes (Riehl & Patzner, 1998), the characid Astyanax bimaculatus (Rizzo et al, 2002), and sturgeons (Psenicka et al, 2010). Its role in the ecophysiology of fish eggs is not well established.…”

Section: Discussionmentioning

confidence: 98%

“…Apparently, no relationship between jelly coat and egg adhesiveness has been found since it is present in fishes bearing adhesive and non-adhesive eggs (Rizzo et al, 2002). In the sturgeon Acipenser baerii, the jelly coat covers the entire egg and acts like a stopper over the micropyles and is released in the freshwater within one min (Psenicka et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Induced spawning and early ontogeny in hatchery-reared catfish Zungaro jahu (Siluriformes: Pimelodidae)

et al. 2012

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Hatchery-kept catfish jahus Zungaro jahu (Ihering, 1898) were induced to spawn with carp pituitary extract. The telolecithal eggs were round (1.6 ± 0.1 mm in diameter), demersal, free, and covered with a 0.4 mm-thick jelly coat. The gonadosomatic index of 2.8 was comparable to that of other Pimelodidae. The number of eggs x g of ova-1 was 804 ± 144. Hatching occurred 14.5 h after fertilization, at a temperature of 27.3 ± 0.4º C. The newly-hatched embryos measured 3.9-4.3 mm of total length (TL). At 18 h post-hatching (HPH; 5.3 ± 0.1 mm TL), the retina was pigmented, the mouth opened and dorsoflexion of the notochord had initiated. At 36 HPH (6.4 ± 0.2 mm TL), fusiform chromatophores were vertically arranged in the primordial fin fold and the notochord was dorsoflexed. The yolk sac was almost exhausted by 48 HPH (7.3 ± 0.2 mm TL). At 128 HPH (8.6 ± 0.6 mm TL) the pectoral, dorsal, adipose, caudal, anal, and pelvic fins were readily observable whereas the primordial fin fold was no longer visible. At 224 HPH (16.6 ± 2.5 mm TL), the metamorphosis was completed and the larvae had acquired the juvenile appearance.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Induced spawning and early ontogeny in hatchery-reared catfish Zungaro jahu (Siluriformes: Pimelodidae)

et al. 2012

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“…In Acipenser sinensis, although measures of the head, midpiece and flagellum of spermatozoa were similar to those of the species studied here (1.84 ± 0.45 µm in 181 width and 3.27 ± 0.20 µm in length vs. 2.17 ± 0.36 µm and 33.26 ± 2.74 µm, 182 respectively), morphology differs regarding the acrosome inserted in the head and elongated in the presence of a short middle piece (Wei et al, 2007). In Acipenser beriberi, the structure is similar to that of the other species of the genus Acipenser (Psenicka et al, 2008;Psenicka et al, 2010); the midpiece is relatively smaller (1.09 ± 0.42 µm and the flagellum is long (44.75 ± 4.93 µm) . Some species sturgeon may exhibit similar sized sperm, but differ in their structure.…”

Section: Discussionmentioning

confidence: 74%

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

Murgas¹,

Paulino²,

Palhares³

et al. 2017

fisheriessciences

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Abstract:This study was designed to identify and measure the gametes of neotropical fish using ultrastructural analysis. Number, shape and size of micropyle and ultrastructure of egg membrane and of the micropyle canal; the number and length of the longest and shortest ridge in the micropyle region beyond the structures of the sperm cell have been used as taxonomic tool in ichthyology . Gametes of six breeding pairs of Piaractus mesopotamicus, Brycon orbignyanus, Salminus brasiliensis and Prochilodus lineatus were collected, fixed and subjected to scanning electron microscopy for the observation and measurement of the structures. The results showed many similarities between the gametes of the studied species. An array of grooves and folds was observed in the micropyle in all species but not in the Prochilodus lineatus oocytes. The micropyle was funnel-shaped in all species. The oocytes showed a great variation in the diameters of major and minor micropyle ostium, evidencing different reproductive strategies. The sperm cells showed simple structures with ovoide heads, a cylindrical midpiece with a single flagellum and no acrosome. This study may contribute to a better understanding of fish reproductive biology, conservation genetics and breeding studies. Eight hours after the second CECP dose, fish were removed from the aquarium and had their urogenital papilla cleaned and dried with a towel. The collection of gametes began after delicate manual massage of the coelomic wall. This procedure was performed in accordance with the Ethical Principles of Animal Experimentation, Protocol 040/2009 of Lavras Federal University-UFLA. Keywords Ultrastructural analysis of gametesPost-fixation methodology was accomplished in accordance to the methodology employed in the Laboratory of Eletronic Microscopy and Ultrastructural Analysis of Lavras Federal University-UFLA. Samples containing 1mL of semen or 30 oocytes of each species were fixed separately in microtubules containing a solution of 2.5% glutaraldehyde and 2.0% paraformaldehyde in 0.05 M sodium cacodylate buffer, pH 7.2, calcium chloride 0.001 M. The samples were maintained for 24 hours at 4°C. After this, samples were immersed in 1% osmium tetroxide for 4 hours at room temperature and subsequent dehydration through in ascending series of acetone (25%, 50%, 75%, 90% and 100%).Before evaluation in the electron scanning microscope, the oocytes and spermatozoa were dehydrated with a CPD030 critical point instrument. The samples were coated with gold in a SCD 050 vacuum evaporator according to protocols designed in Ultrastructural Analysis and Electronic Microscopy Laboratory of Federal University of Lavras, Brazil. Morphometric analysis of the gametesIn the morphometry of the oocytes, was valued the major diameter (Md), minor diameter (md), total volume and the sperm head width ratio and the major diameter of major micropyle ostium (MSH/MO). In the morphometry of the micropyle (Figure 1) in the oocytes surface, was measured the diameters of the major ostium (MO) and minor...

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“…Eggs of sturgeons including the investigated species, Acipenser gueldenstaedtii and Acipenser baerii, are equipped with multiple micropyles lo-cated at the animal region that allow entry of numerous spermatozoa (CHERR & CLARK 1982;DEBUS et al 2008;PSENICKA et al 2010;¯ELAZOWSKA 2010). Observation of egg surfaces in A. transmontanus and in A. baerii during gamete fusion revealed that after incorporation of a spermatozoon, the contents of cortical granules was released into the space between the oocyte and egg envelope.…”

mentioning

confidence: 99%

“…In response to fertilisation they exocytose their contents from the oocyte surface to the perivitelline space (GINSBURG 1961;CHERR & CLARK 1985;DETTLAFF et al 1993;PSENICKA et al 2010). In teleost fish they are also termed cortical alveoli or cortical vesicles, and are produced by endoplasmic reticulum (ER) and Golgi complexes (SELMAN & WALLACE 1989;HART 1990;KUNZ 2004).…”

mentioning

confidence: 99%

Ultrastructure and Histochemistry of the Periplasm in Oocytes of Sturgeons during Egg Envelope Formation

Żelazowska¹

2014

folia biol (krakow)

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Ultrastructural study on the fertilisation process in sturgeon (Acipenser), function of acrosome and prevention of polyspermy

Cited by 49 publications

References 21 publications

Induced spawning and early ontogeny in hatchery-reared catfish Zungaro jahu (Siluriformes: Pimelodidae)

Induced spawning and early ontogeny in hatchery-reared catfish Zungaro jahu (Siluriformes: Pimelodidae)

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

Ultrastructure and Histochemistry of the Periplasm in Oocytes of Sturgeons during Egg Envelope Formation

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