Ultrastructural study of unencapsulated vertebrate mechanoreceptor terminals facilitated by double staining and resectioning of thick plastic sections

Krauhs, Jane M.; Salinas, Nicolás

doi:10.1016/0165-0270(80)90024-2

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…They were then cut into two or three pieces smaller in area, sectioned for light microscopy (3-pm sections), and stained with p-phenylenediamine and toluidine blue. Selected sections were remounted and sectioned for electron microscopy (silver sections) as described by Krauhs and Salinas (1980). Thick and thin sections were cut tangential to the trachea wall instead of being cut as cross sections, and all pieces were thick-sectioned through the entire thickness of trachealis muscle.…”

Section: Methodsmentioning

confidence: 99%

Morphology of presumptive slowly adapting receptors in dog trachea

Krauhs

1984

Anat. Rec.

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The three-dimensional branching pattern and ultrastructure of afferent myelinated fibers and their terminals located in the trachealis muscle of the dog are described. The afferent endings are believed to be those of the slowly adapting stretch receptors of the trachea. They have structural features typical of mechanoreceptors: distal to the loss of myelin, their shape becomes more irregular and the cytoplasm is filled with mitochondria, glycogen, and osmiophilic bodies. In some places the cell membrane is attached directly to basal lamina without interposition of a Schwann cell. A bundle of unmyelinated fibers accompanies each myelinated fiber and continues for an undetermined distance beyond (luminal to) terminations of the myelinated fiber. The unmyelinated fibers contain many round, clear vesicles and a few dense-cored vesicles and are also attached directly to basal lamina in places. Three-dimensional reconstruction of three receptors revealed three quite different branching patterns, but all included apparent rings as part of more or less contorted terminal regions (some neurons apparently having more than one terminal region). No obvious structural basis for the activation of receptors by transverse but not longitudinal stretch was found.

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Section: Methodsmentioning

confidence: 99%

Morphology of presumptive slowly adapting receptors in dog trachea

Krauhs

1984

Anat. Rec.

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Mircofibrils in the Aorta

Krauhs

1983

Connective Tissue Research

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Ruthenium red was used to stain microfibrils in rat aorta after incubation of the tissues with or without one of the enzymes trypsin, collagenase, phospholipase C, chondroitinase ABC, hyaluronidase or neuraminidase, or the reducing agent dithiothreitol. Microfibrils exhibiting periodicity of ruthenium red binding were associated with elastic laminae and collagen fibrils and appeared to attach these structures to each other as well as to basal lamina. Microfibrils in rat and human aorta demonstrated fibronectinlike immunoreactivity, therefore fibronectin may be a component of aorta microfibrils and important in the architecture of blood vessels.

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6 Final Report on the Safety Assessment ofp-Phenylenediamine

1985

Journal of the American College of Toxicology

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p-Phenylenediamine is a cosmetic hair dye intermediate used in permanent hair coloring products at concentrations of up to 5 percent (diluted 1:1 with an oxidizing agent prior to application). The extensive animal toxicity test data on p-Phenylenediamine and permanent cosmetic hair dyes containing this compound show that the degree of toxicity varies with concentration, test system and animal species. Animal data support a conclusion that this compound is neither a teratogen nor a carcinogen. Epidemiological data also support that hair dyes containing this ingredient are not carcinogenic. p-Phenylenediamine is a sensitizer and some persons may be sensitized under intended conditions of use. For those persons not sensitized, it is concluded that p-Phenylenediamine is safe as a hair dye ingredient at the current concentrations of use.

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Ultrastructural study of unencapsulated vertebrate mechanoreceptor terminals facilitated by double staining and resectioning of thick plastic sections

Cited by 3 publications

References 14 publications

Morphology of presumptive slowly adapting receptors in dog trachea

Morphology of presumptive slowly adapting receptors in dog trachea

Mircofibrils in the Aorta

6 Final Report on the Safety Assessment ofp-Phenylenediamine

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