Ultrastructural Study of Taste Buds at Rest and After Stimulation, and Comparative Study Between Type III Cell and Merkel Cells

Ciges, M; Flores, L. Díaz; González, M; Rama, J

doi:10.3109/00016487609119952

Cited by 18 publications

(5 citation statements)

References 17 publications

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“…Typical synapses with the postsynaptic density in the taste bud cell were sometimes seen in the mid-and basal regions of the taste buds (Figs. 3,9), Cell-nerve contacts, lacking post-synaptic densities but with clear synaptic-type vesicles present in the immediately adjacent taste bud cell cytoplasm and in the nerve fiber, were also seen ( Figs. 3, 7, 8,10).…”

Section: Resultsmentioning

confidence: 94%

“…It seems reasonable then to suppose that at least some of the receptors for the primary transduction of chemical stimuli must be found on these microvilli. In addition, it is possible that cells respond to stimuli with a change in volume, thus altering the diameter of the taste pore (9) and allowing access to other parts of the taste bud ceil. However, it cannot be excluded that the apical barrier may be permeable to some substances (7,11), thus allowing direct contact with nerve fibers, which rise in many cases almost to the apex of the taste bud, or non-apical portions of taste cells.…”

Section: Discussionmentioning

confidence: 99%

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Fine structure of taste buds in te human fungiform papilla

Arvidson

Cottler‐Fox

Friberg

1981

European J Oral Sciences

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– The fine structure of taste buds on fungiform papillae from man was examined with particular reference to different cell types and to cell‐nerve contactsw. Most of the elongated cells of the taste bud extended from the basal lamina to the apical pore, terminating in irregular microvilli. The cells contained clear apical vesicles, mitochondria, filament bundles and Golgi cisternae. Unmyelinated nerve fiberscontaining mitocliondria as both dense‐cored and clear synaptic‐type vesicles, were scartered throughout the whole taste bud but were most numerous at the base. The nervefibers were either in simple appositional contact or in mesaxonal contact with the taste bud cell. Typical synapses with the postsynaptic densities in the taste bud cell were sometimes seen. In addition, single taste bud cells were observed to have more than one type of contact with nerve fibers. As in the Old World monkey Cynomolgus it was not possible to distinguish different cell types described earlier in other species.

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Section: Resultsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Fine structure of taste buds in te human fungiform papilla

Arvidson

Cottler‐Fox

Friberg

1981

European J Oral Sciences

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“…The results of recent investigations in the rabbit have suggested that at least some of these vesicles contain serotonin, a potential neurotransmitter (Fujimoto, 1982; see also Nada and Hirata, 1975;Takeda, 1977;Takeda et al, 1981). The larger of these vesicles have also been compared to Merkel cell vesicles, on the basis of their size and orientation to intraepithelial nerve processes (Ciges et al, 1976;Ide and Munger, 1980). Others have speculated that the granules may be related to a neurotrophic substance(s) (Farbman, 1965;Zahm and Munger, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…Electron microscopists have shown that taste buds contain either two (DeLorenzo, 1958;NemetschekGansler and Ferner, 1964;Farbman, 1965;Graziadei, 1969;Uga, 1969;Kinnamon, 1982;Zahm and Munger, 1983) or three differentiated cell types (Murray et al, 1969;Fujimot0 and Murray, 1970;Sangiacomo, 1970;Murray, 1971Murray, , 1973Murray and Murray, 1971;Fujimoto, 1975;Paran et al, 1975;Takeda and Hoshino, 1975;Takeda, 1976;Ciges et al, 1976;Gomez-Ramos and Rodriguez-Echandia, 1979). In earlier electron microscope investigations on adult monkey taste buds, there was little clear evidence for more than one cell type in fungiform (Arvidson et al, 1981) and circumvallate papillae (Murray and Murray, 1960).…”

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confidence: 99%

Structure of taste buds in foliate papillae of the rhesus monkey, Macaca mulatta

Farbman¹,

Hellekant

Nelson³

1985

Am. J. Anat.

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Taste buds in foliate papillae of the rhesus monkey were examined by electron microscopy. Three distinct cell types were identified. Type I cells were narrow elongated cells containing an oval nucleus, bundles of intermediate filaments, several Golgi bodies, and characteristic apical membrane-bounded dense granules. These cells exhibited morphological variations: some had a moderately dense cytoplasm, perinuclear free ribosomes, and flattened sacs of rough endoplasmic reticulum; others had a more lucent cytoplasm, dilated irregular rough endoplasmic reticulum, lysosome-like dense bodies, and lipid droplets. Type II cells typically contained a spherical, pale nucleus, a prominent nucleolus, supranuclear and infranuclear Golgi bodies, mitochondria with tubular cristae, and one or two centrioles. This cell type, too, showed some variation in the relative amounts of ribosomes and smooth endoplasmic reticulum, which varied inversely with each other. Type III cells were characterized by a clear apical cytoplasm essentially devoid of ribosomes and containing microtubules. In a few type III cells, the peri- and infranuclear regions contained many ribosomes and some rough endoplasmic reticulum. In most Type III cells, there were large numbers of dense and clear vesicles in the peri- and infranuclear regions; some of the vesicles were grouped in synapse-like arrangements with adjacent nerves. The morphological variations exhibited by all three cell types could be accounted for by age differences in each of the cells. This would be consistent with the notion that cell renewal occurs in each of the three cell populations.

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“…Recent experiments in mammals indicate that both taste cells and Merkel cells originate from local epithelium rather than migrating in from remote sources like the neural crest [12,16,33]. In addition to sharing a local origin, both Merkel cells and type III taste receptor cells have dense core vesicles, neuronspecific enolase and keratins 8 and 19 [5,9,39,40]. While this is suggestive, there is little additional evidence linking Merkel cells or Merkel-like cells with mammalian taste buds [18,30].…”

Section: Introductionmentioning

confidence: 99%

The distribution and origin of keratin 20-containing taste buds in rat and human

Zhang

Oakley

1996

Differentiation

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Sections of tissues containing lingual and extra-lingual taste buds were evaluated with monoclonal antibodies against cytokeratins. In the caudal third of the rat's tongue, keratin 20 immunoreactivity was restricted to taste buds, whereas keratins 7, 8, 18, and 19 were expressed in vallate and foliate taste buds and in cells of salivary ducts that merge with these taste epithelia. Hence, antibodies against keratin 20 most clearly distinguished differentiated taste cells from all other cells. In rat epiglottis, taste buds and isolated bipolar cells were keratin-20-positive. In rat nasopalatine papilla and palate, antibodies against keratin 20 identified Merkel cells, none of which was near to the keratin-20-negative taste buds. Nor were Merkel cells present at epiglottal taste buds or the keratin-20-negative fungiform taste buds or elsewhere in rat tongue. Hence, Merkel cells make no contribution to rat fungiform, epiglottal, nasopalatine, or palatal taste buds. Human and rat keratin-20-positive tissues are reported to be endodermal derivatives with the exception of Merkel cells and luminal urothelial cells. In rats the distribution of keratin-20-positive taste buds was in full agreement with the classical view that the posterior third of the tongue is derived from endoderm (keratin-20-positive taste buds), whereas the anterior two-thirds of the tongue is derived from stomadeal ectoderm (keratin-20-negative taste buds). The equally intense keratin 20 immunoreactivity of human fungiform and vallate taste buds violates this traditional rostro-caudal segregation and suggests that endodermally derived tissues may be present in the tip of the human tongue.

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Ultrastructural Study of Taste Buds at Rest and After Stimulation, and Comparative Study Between Type III Cell and Merkel Cells

Cited by 18 publications

References 17 publications

Fine structure of taste buds in te human fungiform papilla

Fine structure of taste buds in te human fungiform papilla

Structure of taste buds in foliate papillae of the rhesus monkey, Macaca mulatta

The distribution and origin of keratin 20-containing taste buds in rat and human

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