Ultrastructural study of nucleolus reorganisation in telophase in a green alga.

Ashraf, Mohammad Javad; Godward, M. B. E.

doi:10.1508/cytologia.53.447

Cited by 1 publication

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“…However, several examples in different species of animals and plants demonstrate that specially fibrillar, but sometimes granular material, may persist throughout the cellular cycle, including metaphase and anaphase. These evidences support the possibility that nucleolar material is not completely synthesized in the chromosomal NOR but that a pre-existing material is also necessary and used as was already mentioned by Ashraf and Godward (1988).…”

Section: Discussionsupporting

confidence: 78%

Meiosis Aspects and Nucleolar Activity in Triatoma vitticeps (Triatominae, Heteroptera)

et al. 2006

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Some aspects of both the nucleolar organizer activity and meiosis were studied in the testes of Triatoma vitticeps (Heteroptera, Triatominae). The techniques used included squashing followed by lacto-acetic orcein staining, silver-ion impregnation, fluorescent banding (CMA3, Quinacrine mustard and DAPI) and fluorescent in situ hybridization (FISH). A close relationship between heterochromatin and nucleolus in testicular cells was observed. During meiosis, the silver-ion impregnation pattern varied. At metaphase plate, a small body appeared apart from the chromosomes. In the spermatids this small body was seen in preparations stained with orcein and silver- ion impregnation but not with fluorochromes or FISH. These characteristics combined suggest that these corpuscles represent a source of ribonucleoproteins (RNP)-RNA and specific nucleolar proteins. Silver-ion impregnation and (FISH) revealed nucleolar organizer activity in two metaphase sex chromosomes (X). These results indicate that, in these species, nucleolar organizer regions (NORs) are located in the sex chromosomes, X chromosomes were CMA3+ and Y chromosome was DAPI+.

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Section: Discussionsupporting

confidence: 78%