Ultrastructural study and description of Ordospora colligata gen. et sp. nov. (microspora, ordosporidae fam. nov.), a new microsporidian parasite of Daphnia magna (Crustacea, Cladocera)

Larsson, J.I. Ronny; Ebert, Dieter; Vávra, Jiří

doi:10.1016/s0932-4739(97)80055-7

Cited by 46 publications

(61 citation statements)

References 26 publications

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“…The sporont walls are initiated identically over wide areas in both species (Larsson et al 1998) as it also is in F. montana (Canning et al 2001: Fig. 12).…”

Section: Phenetic Relationshipsmentioning

confidence: 83%

“…Here we report on 2 microsporidian species infecting the planktonic crustacean Daphnia magna, Hamiltosporidium tvaerminnensis sp. nov., described in this paper, and Flabelliforma magnivora (Larsson et al 1998), which has recently been placed in the new genus Neoflabelliforma (Morris and Freeman, 2010). These species show clear differences in their cytology, biogeography, ecology and epidemiology, yet sequence data show that they are very close to each other.…”

Section: Introductionmentioning

confidence: 84%

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Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

et al. 2010

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Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov. We describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha-and betatubulin. This parasite was previously identified as Octosporea bayeri Jírovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia -host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages of H. tvaerminnensis have isolated nuclei; merozoites are formed by binary fission or by the cleaving of a plasmodium with a small number of nuclei. A sporogonial plasmodium with isolated nuclei yields 8 sporoblasts. Elongated spores are generated by the most finger-like plasmodia. The mature spores are polymorphic in shape and size. Most spores are pyriform (4·9-5·6 × 2·2-2·3 μm) and have their polar filament arranged in 12-13 coils. A second, elongated spore type (6·8-12·0 × 1·6-2·1 μm) is rod-shaped with blunt ends and measures 6·8-12·0 × 1·6-2·1 μm. The envelope of the sporophorous vesicle is thin and fragile, formed at the beginning of the sporogony. Cytological and molecular comparisons with Flabelliforma magnivora, a parasite infecting the same tissues in the same host species, reveal that these two species are very closely related, yet distinct. Moreover, both cytological and molecular data indicate that these species are quite distant from F. montana, the type species of the genus Flabelliforma. We therefore propose that F. magnivora also be placed in Hamiltosporidium gen. nov.

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“…The sporont walls are initiated identically over wide areas in both species (Larsson et al 1998) as it also is in F. montana (Canning et al 2001: Fig. 12).…”

Section: Phenetic Relationshipsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 84%

Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

et al. 2010

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“…Other members of the S. perivermis subclade, O. colligata and the Encephalitozoon group, also develop in isolation from their hosts' tissues. However, their separation is achieved by means of a membrane-bound parasitophorous vacuole (host origin) instead of a vesicle (24,31). The host-parasite interface varies considerably for other members of clade IV, as some (i.e., Endoreticulatus species) develop in parasitophorous vacuoles, while others (i.e., Nosema) develop in direct contact with the host tissues (5).…”

Section: Discussionmentioning

confidence: 99%

Microsporidian Infection in a Free-Living Marine Nematode

Ardila-Garcia

Fast

2012

Eukaryot Cell

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Microsporidia are unicellular fungi that are obligate endoparasites. Although nematodes are one of the most abundant and diverse animal groups, the only confirmed report of microsporidian infection was that of the "nematode killer" (Nematocida parisii). N. parisii was isolated from a wild Caenorhabditis sp. and causes an acute and lethal intestinal infection in a lab strain of Caenorhabditis elegans. We set out to characterize a microsporidian infection in a wild nematode to determine whether the infection pattern of N. parisii in the lab is typical of microsporidian infections in nematodes. We describe a novel microsporidian species named Sporanauta perivermis (marine spore of roundworms) and characterize its infection in its natural host, the freeliving marine nematode Odontophora rectangula. S. perivermis is not closely related to N. parisii and differs strikingly in all aspects of infection. Examination by transmission electron microscopy (TEM) revealed that the infection was localized in the hypodermal and muscle tissues only and did not involve the intestines. Fluorescent in situ hybridization (FISH) confirmed infection in the muscle and hypodermis, and surprisingly, it also revealed that the parasite infects O. rectangula eggs, suggesting a vertical mode of transmission. Our observations highlight the importance of studying parasites in their natural hosts and indicate that not all nematode-infecting microsporidia are "nematode killers"; instead, microsporidiosis can be more versatile and chronic in the wild.

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“…Glugoides intestinalis and Ordospora colligata are microsporidian parasites of D. magna (Larsson et al 1996(Larsson et al , 1997. These unicellular parasites transmit horizontally via infectious spores and infect epithelial cells in the upper half of the gut of their host.…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

“…bayeri, yet its SSUrRNA, ITS and 5k end of the LSU are identical to Flabelliforma magnivora (GenBank Accession no. AY649786), another parasite of D. magna (Larsson et al 1998). Currently the taxonomic relationship between these parasites is not resolved as there are no ultrastructural data available on Oc.…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

Quantitative PCR to detect, discriminate and quantify intracellular parasites in their host: an example from three microsporidians in Daphnia

Refardt

Ebert

2006

Parasitology

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Reliable detection, discrimination and quantification of parasites are important for host-parasite studies and diagnostics. Microsporidial infections are problematic in this respect. Their discrimination and quantification using light microscopy is difficult because spores are the only light microscopically visible form of the parasite and they offer few distinct characters. We developed a quantitative PCR (qPCR) assay based on SYBR Green chemistry to quantify the microsporidia Glugoides intestinalis, Octosporea bayeri and Ordospora colligata in their host, the freshwater crustacean Daphnia magna. The assay allows the quantification of infection intensities in whole animals and is more than an order of magnitude more sensitive than light microscopy. Sampling and DNA extraction account for more than 90 % of the residual variance in infection intensity data and this variance considerably impairs the resolution of qPCR. Where higher resolution is required, we propose using the ratio of parasite to host DNA as the measure of infection intensity. We show that this measure is robust and greatly improves resolution of qPCR. Additionally, this method can be applied to compare samples of unequal volume.

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Ultrastructural study and description of Ordospora colligata gen. et sp. nov. (microspora, ordosporidae fam. nov.), a new microsporidian parasite of Daphnia magna (Crustacea, Cladocera)

Cited by 46 publications

References 26 publications

Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp. nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

Microsporidian Infection in a Free-Living Marine Nematode

Quantitative PCR to detect, discriminate and quantify intracellular parasites in their host: an example from three microsporidians in Daphnia

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