Ultrastructural studies on the sporogenous tissue and anther wall of Leucojum aestivum (amaryllidaceae) in different developmental stages

Ekici, Nuran; Dane, Feruzan

doi:10.1590/s0001-37652012005000061

Cited by 6 publications

(5 citation statements)

References 22 publications

(17 reference statements)

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“…Increase of RNA content in the middle layer and endothecium can be related with pollen nutrition. The ultrastructure of anther wall cells also justifies the results of histological observations (Ekici & Dane 2012).…”

Section: Discussionsupporting

confidence: 65%

“…In recently done studies in Leucojum aestivum, polarity was determined in ultrastructures of PMC (Pollen mother cells), sporogenous tissue cells and the microspores which were ready for pollen mitosis, in the distribution of organelles (Ekici & Dane 2012), in the synergids and egg cells of female gametophyte and in the active (functional) megaspore (Ekici & Dane 2008). In this study, some of the histochemical features of the anther wall in Leucojum aestivum were tried to explain during pollen development.…”

Section: Introductionmentioning

confidence: 99%

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Some histochemical features of anther wall of Leucojum aestivum (Amaryllidaceae) during pollen development

Ekici

Dane

2012

Biologia

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In this study, polysaccharide and RNA contents of anthers were investigated on different phases of sporogenesis by using light microscopy techniques from histological and cytological point of view in Leucojum aestivum. Paraffin and semi-thin sections of anthers were stained with toluidine blue and PAS. Anthers were tetrasporangiate. The wall of the anther consists of an epidermis, endothecium, middle layer and glandular tapetum. During one nucleated microspore and mature pollen phase microspores and tapetum cells began to degenerate and they were become very rich of RNA in L. aestivum. And also RNA content was increased in endothecium and middle layer cells except the epidermis cells of anther wall. An increase in RNA content indicates cell activation. Polysaccharides were not seen in young anther wall but they were seen in older ones. They were generally condensed in the cell walls and especially in the cell walls of vascular bundles of connective tissue. This could be thought that insoluble polysaccharides were used in metabolic events in early developmental stages. Appearance of polysaccharides in late phases was indicated that polysaccharides were used in the formation of cuticule and differentiation of endothelium cell walls.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Some histochemical features of anther wall of Leucojum aestivum (Amaryllidaceae) during pollen development

Ekici

Dane

2012

Biologia

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“…In this study, the sorghum anther developmental process exhibited both similarities and differences with other plant species. We have found that later stages of anther development are much alike across a broad range of different plant species while the early stages vary somewhat among different species (Sanders et al 1999 ; Zhang et al 2011 ; Ekici and Dane 2012 ; Wang et al 2012 ; Xu et al 2014 ; Tsou et al 2015 ; Browne et al 2018 ; Chaban et al 2020 ). The anther development in sorghum resembles mostly to those of maize anthers (Wang et al 2012 ; Moon et al 2013 ; Tsou et al 2015 ), followed by those in wheat and rice (Zhang et al 2011 ; Browne et al 2018 ).…”

Section: Discussionmentioning

confidence: 98%

Morphological analysis and stage determination of anther development in Sorghum [Sorghum bicolor (L.) Moench]

Laza

Kaur-Kapoor

Xin³

et al. 2022

Planta

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Main Conclusion The characteristics of sorghum anthers at 18 classified developmental stages provide an important reference for future studies on sorghum reproductive biology and abiotic stress tolerance of sorghum pollen. Abstract Sorghum (Sorghum bicolor L. Moench) is the fifth-most important cereal crop in the world. It has relatively high resilience to drought and high temperature stresses during vegetative growing stages comparing to other major cereal crops. However, like other cereal crops, the sensitivity of male organ to heat and drought can severely depress sorghum yield due to reduced fertility and pollination efficiency if the stress occurs at the reproductive stage. Identification of the most vulnerable stages and the genes and genetic networks that differentially regulate the abiotic stress responses during anther development are two critical prerequisites for targeted molecular trait selection and for enhanced environmentally resilient sorghum in breeding using a variety of genetic modification strategies. However, in sorghum, anther developmental stages have not been determined. The distinctive cellular characteristics associated with anther development have not been well examined. Lack of such critical information is a major obstacle in the studies of anther and pollen development in sorghum. In this study, we examined the morphological changes of sorghum anthers at cellular level during entire male organ development processes using a modified high-throughput imaging variable pressure scanning electron microscopy and traditional light microscopy methods. We divided sorghum anther development into 18 distinctive stages and provided detailed description of the morphological changes in sorghum anthers for each stage. The findings of this study will serve as an important reference for future studies focusing on sorghum physiology, reproductive biology, genetics, and genomics.

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“…the experimental samples are of high quality and the data meet the requirements, and the test data is accurate. Several studies have shown that the MFA in the annual rings near the young timber of the medullary heart is usually large, and it tends to decrease with the increase in tree age, and then remains relatively stable [23][24][25].…”

Section: Microfibril Angle (Mfa)mentioning

confidence: 99%

Intratree Variation in Viscoelastic Properties of Cell Walls of Masson Pine (Pinus Massoniana Lamb)

Cai¹,

Guo²,

Li³

2022

Journal of Renewable Materials

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In this study, Pinus massoniana Lamb at different heights, across the annual rings, and between earlywood and latewood was measured by X-ray diffraction and the chemical composition was analyzed by chemical treatment. Results indicated that the microfibril angle (MFA) decreased and the chemical composition changed little with the increase in height from 1 m to 9 m. In the radial direction, the MFA decreased and the chemical composition changed little with an increase in annual rings. The cellulose content of latewood was higher than that of earlywood. The viscoelastic changes of wood cell walls at different heights, across the annual rings by the method of quasi-static nanoindentation and dynamic modulus mapping techniques. Results indicated that the wood cell walls' elastic modulus increased, and the creep rate and creep compliance decreased with the increase in height; The elastic modulus and hardness increased with the annual rings. The cell walls' storage modulus increased and the loss modulus gradually decreased with an increase in height; the storage modulus gradually increased and the loss modulus decreased with the annual rings.

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Ultrastructural studies on the sporogenous tissue and anther wall of Leucojum aestivum (amaryllidaceae) in different developmental stages

Cited by 6 publications

References 22 publications

Some histochemical features of anther wall of Leucojum aestivum (Amaryllidaceae) during pollen development

Some histochemical features of anther wall of Leucojum aestivum (Amaryllidaceae) during pollen development

Morphological analysis and stage determination of anther development in Sorghum [Sorghum bicolor (L.) Moench]

Intratree Variation in Viscoelastic Properties of Cell Walls of Masson Pine (Pinus Massoniana Lamb)

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