Ultrastructural studies on the secretory mechanism of goblet cells in the rat jejunal epithelium.

Kurosumi, Kazumsa; Shibuichi, Izumi; Tosaka, Hisami

doi:10.1679/aohc1950.44.263

Cited by 26 publications

(9 citation statements)

References 24 publications

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“…A similar phenomenon was observed in the goblet cells of the mouse colonic mucosa (Ichikawa et al, 1987), and in rat parotid acinar cells the secretory material was found to be secreted to the lumen through deep invaginations (Sahara, 1985). A similar, but less dramatic situation was observed during the extrusion of mucus from the goblet cells of the rat jejunum (Kurosumi et al, 1981); the most superficial mucous droplets were released by exocytosis, but more frequently the deeply located droplets fused to each other and a huge vacuole was formed in the apical cytoplasm. It was supposed that during the secretion of the mucous granules from the goblet cells, three different mechanisms must exist for protein secretion: classical exocytosis, typical apocrine secretion, and modified apocrine secretion.…”

supporting

confidence: 67%

Exocytosis from rat submandibular granular tubules during cyclocytidine stimulation shows unusual features, including changes in the granule membrane

Thomopoulos

Garrett

Proctor

et al. 1996

Microsc. Res. Tech.

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supporting

confidence: 67%

Exocytosis from rat submandibular granular tubules during cyclocytidine stimulation shows unusual features, including changes in the granule membrane

Thomopoulos

Garrett

Proctor

et al. 1996

Microsc. Res. Tech.

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“…The disappearance of the membranes of the adjacent mucous granules that enabled almost instantaneous evacuation of the whole mucus load was observed in numerous cells. This most rapid way of mucus discharge was previously described in stimulated goblet cells of the small intestine [8][9][10].…”

Section: Discussionsupporting

confidence: 67%

Comparison of the Effect of a High and Low Dose of Atropin on the Ultrastructure of the Tracheal Epithelium

Konrádová

J²,

Zocová³

et al. 1996

Respiration

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The ultrastructure of rabbit tracheal epithelium was studied 20 min after injection of 0.04 and 0.5 mg of atropin. The tracheae were lined with altered ciliated pseudostratified columnar epithelium. The degree of damage to the epithelial cells was dose dependent. The injury to the tracheal epithelium due to treatment with 0.04 mg of atropin was considered mild. The administration of the higher dose of atropin caused moderately severe epithelial damage. Both experimental groups exhibited morphological signs of impaired self-cleaning ability.

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“…In addition to its direct association with the granule membranes, mCLCA3 was also detected in the electron-dense material that buds from the luminal surface of the granule membranes during granule fusion and maturation. Although precise composition and function of this material are unknown, it has been suggested that it represents excess membrane proteins shed during fusion of the goblet granules (Swift and Mukherjee 1978;Kurosumi et al 1981;Wille 1990). …”

Section: Discussionmentioning

confidence: 99%

The Murine mCLCA3 (Alias gob-5) Protein Is Located in the Mucin Granule Membranes of Intestinal, Respiratory, and Uterine Goblet Cells

Leverkoehne¹,

Gruber²

2002

J Histochem Cytochem.

108

146

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The putative anion channel mCLCA3 (alias gob-5) is the third murine member of the recently discovered family of calcium-activated chloride channels (CLCA family). Preliminary data suggest that mCLCA3 may play a significant role in diseases with secretory dysfunctions, including asthma and cystic fibrosis. In this study, the mCLCA3 protein was characterized biochemically and its cellular and subcellular distribution pattern was established in normal murine tissues. Polyclonal rabbit antibodies were generated and affinity-immunopurified using synthetic oligopeptides corresponding to the extracellular amino terminus of the mCLCA3 polypeptide. After in vitro translation and glycosylation, proteinase K protection assay, and heterologous expression in COS-7 or HEK 293 cells, SDS-PAGE and immunoblotting revealed a protein structure similar to that of previously characterized CLCA proteins. A systematic light, confocal laser scanning, and transmission electron microscopic immunolocalization study, including virtually all murine tissues, identified the mCLCA3 protein exclusively associated with mucin granule membranes of gastrointestinal, respiratory, and uterine goblet cells and other mucin-producing cells. The results suggest that mCLCA3 may be involved in the synthesis, condensation, or secretion of mucins.

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Ultrastructural studies on the secretory mechanism of goblet cells in the rat jejunal epithelium.

Cited by 26 publications

References 24 publications

Exocytosis from rat submandibular granular tubules during cyclocytidine stimulation shows unusual features, including changes in the granule membrane

Exocytosis from rat submandibular granular tubules during cyclocytidine stimulation shows unusual features, including changes in the granule membrane

Comparison of the Effect of a High and Low Dose of Atropin on the Ultrastructure of the Tracheal Epithelium

The Murine mCLCA3 (Alias gob-5) Protein Is Located in the Mucin Granule Membranes of Intestinal, Respiratory, and Uterine Goblet Cells

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