Ultrastructural studies on the natural leaf senescence of Cinnamomum camphora

Abstract

The process of natural leaf senescence of Cinnamomum camphora (C. camphora)-a commercial tree in Asia, was investigated, focusing on changes in cellular ultrastructure, epicuticular wax, and stoma. The changes to mesophyll cells in a senescing leaf predominantly include degradation of the following cellular components: cytoplasm, the central vacuole, small vacuoles, and vesicles with a diameter smaller than 400 nm, which are involved in the degradation of chloroplasts. The sequence of change in epicuticular wax during leaf senescence was different from those in herbaceous plants by atomic force microscope and scanning electron microscopic analysis. Comparing with maturation leaves, senescing leaves develop a wider aperture in their stoma, which would delay the leaf senescence of C. camphora.

Elucidation of microstructural changes in leaves during senescence using spectral domain optical coherence tomography

“…1(b)). Similar findings also can be found in leaves affected by pathogen attacks, which leads to senescence 9,33 . Thus, it can be hypothesized that senescence due to pathogen attacks and autumn phenology might have similar structural alterations.…”

Ghd2,aCONSTANS-like gene, confers drought sensitivity through regulation of senescence in rice

“…Normally grown Ghd2 -OE and WT' plants were sampled at the same time as controls. TEM and SEM were conducted as described previously ( Cao et al , 2013 ). The samples were freeze-dried and metabolites were measured according to the method previously described in detail ( Chen et al , 2013 b ).…”

“…Most CO -like genes containing a CCT domain are reported to be involved in the control of flowering time in various plant species. In rice, several such genes control the rice heading date, including Hd1 , OsCOL3 , OsCOL4 , Ghd7 , DTH2 , OsCOL10 , OsCCT01 , OsCCT11 , and OsCCT19 ( Yano et al , 2000 ; Kim et al , 2008 ; Xue et al , 2008 ; Lee et al , 2010 ; Wu et al , 2013 ; Zhang et al , 2015 ; Tan et al , 2016 ). To date, only a few members of this family have been identified as regulators of abiotic stress responses.…”

Study on Extraction of Peanut Protein and Oil Bodies by Aqueous Enzymatic Extraction and Characterization of Protein

“…Embedded samples were cut into ultrathin sections (50-70 nm thick) with an ultramicrotome (UC5; Leica, Wetzlar, Germany). Sections were mounted on copper grids, stained with 2% uranyl acetate and Reynold's lead citrate, and then examined with a transmission electron microscope (TEM) (H-7650; Hitachi Chiyoda-ku, Tokyo, Japan), operated at an accelerating voltage of 80 kV [17].…”