Ultrastructural Studies on the Lymphatic Anchoring Filaments

Leak, Lee V.; Jf, Burke

doi:10.1083/jcb.36.1.129

Cited by 353 publications

(165 citation statements)

References 18 publications

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“…Lymphatic anchoring filaments (Leak and Burke, 1968) and suspensory ligament of the ocular lens (Raviola, 1971) are oxytalan fibers, or elastin-free MFs. Their roles not in elasticity but in architectural and mechanical stiffness has been emphasized (Ramirez and Pereira, 1999;Kielty et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Late Deposition of Elastin to Vertical Microfibrillar Fibers in the Presumptive Dermis of the Chick Embryonic Tarsometatarsus

Yamazaki

Sejima

Yuguchi

et al. 2007

The Anatomical Record

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Fibrillin microfibrils are integral components of elastic fibers and serve as a scaffold for elastin deposition. However, microfibrillar fibers (MFs) are not necessarily committed to develop into so-called elastic fibers. In dermis, elastin-free oxytalan MFs originating from the dermoepidermal junction are continuous to elaunin-type MFs (with a small amount of elastin) in the deeper papillary dermis, whereas the reticular dermis contains elastic fibers, or MFs embedded largely in elastin. In this study, we have investigated temporospatial patterns of elastin deposition on the MFs in tarsometatarsal presumptive dermis. While the earliest expression of elastin was demonstrated immunohistochemically as early as embryonic day 4 (ED4) in the wall of cardiac outflow and pharyngeal arch arteries, its deposition in the tarsometatarsus was first detected at ED6 in the deeper mesenchyme and at ED13 in the subectodermal mesenchyme. In the latter tissue, MFs had been organized perpendicularly to the covering ectoderm by ED4, well before an overt accumulation of collagenous matrix. Elastin deposition was observed initially in a punctate manner at ED13 and afterward became continuous along MFs. However, a characteristic spaced array of subectodermal vertical MFs was disorganized by ED17. These findings suggest that elastin deposition in the subectodermal MFs is not deployed by continuous, orderly propagation from elastic fibers in the deeper mesenchyme but occurs de novo in multiple foci along vertical MFs. Moreover, the present chronology of elastin deposition indicates that subectodermal, elastin-free MFs function as a transient, but primary fibrous structure in the presumptive dermis before the accumulation of collagenous matrix.

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Section: Discussionmentioning

confidence: 99%

Late Deposition of Elastin to Vertical Microfibrillar Fibers in the Presumptive Dermis of the Chick Embryonic Tarsometatarsus

Yamazaki

Sejima

Yuguchi

et al. 2007

The Anatomical Record

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“…They appear to connect endothelial cells to elastic fibers in the subendothelial matrix of the mouse aorta [10] and of lymphatic capillaries [11,12]. In the mouse aortic media the elastic laminae are linked to smooth muscle cells by bundles of microfibrils [13].…”

Section: Introductionmentioning

confidence: 99%

Cell adhesion and integrin binding to recombinant human fibrillin‐1

et al. 1996

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Fibrillin-1 is a major constituent of tissue microfibrfls that occur in most connective tissues, either in close association with or independent of elastin. To test possible cell-adhesive functions of this protein, we used recombinant human fibrlllin-1 polypeptides produced in a mammalian expression system in cell attachment and solid-phase integrin binding assays. necessitates the use of buffers containing denaturing agents and cannot separate fibrillin-1 and fibrillin-2 [18], we chose to use recombinant human fibrillin-1 polypeptides for a study of possible cell-adhesive functions. These human fibrillin-1 peptides were produced in a mammalian expression system and purified under non-denaturing conditions [19]. Extensive characterization indicated correct folding [19]. Using an experimental approach already applied to characterize cell-adhesive and integrin-binding functions of fibulins [20], laminin [21] and collagen VI [22], we demonstrate the ability of cells to adhere to fibrillin-1 via an RGD motif located in the fourth 8-cysteine domain. In addition we identify integrin aV[33 as a major cellular receptor for this site.

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“…This might suggest that microfibrils are found exclusively in the sites where elastogenesis should take place; however, it has been shown that fibrillin microfibrils devoid of amorphous elastin also occur in nonelastic, fully developed tissues. Oxytalan fibers in the periodontal ligament (Fullmer and Lillie, 1958;Carmichael and Fullmer, 1966), lymphatic anchoring filaments (Leak and Burke, 1968), and suspensory ciliary ligament of the ocular lens (Inoue and Leblond, 1986;Wright et al, 1994) form bundles of elastin-free microfibrils. A fibrillar system in the dermis is also known to contain elastin-free microfibrils in its superficial region (Cotta-Pereira et al, 1976, 1977.…”

mentioning

confidence: 99%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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In this study, a unique fiber system in the subectodermal mesenchyme of the chick limb bud was visualized immunohistochemically with the use of a novel monoclonal antibody termed "FB1." This antibody stained a subset of extracellular fibers in the embryonic mesenchyme. Among the fibers visualized, those running perpendicularly to the limb bud ectoderm became progressively prominent in their thickness and length, and organized into a parallel array in the subectodermal region. This fiber system was distinct from that of major collagens, fibronectin, or tenascin. A molecule immunoprecipitated with FB1 comigrated with JB3 antigen, or chicken fibrillin-2. The fibers visualized immunohistochemically by FB1 and JB3 were indistinguishable from each other, and ultrastructurally appeared to be bundles composed of tubular-like microfibrils that originated directly from the ectodermal basal lamina. They lacked the amorphous deposits that are characteristic of elastin. A similar array of subectodermal fibers was also found in the developing axilla and some truncal regions, again well before the development of a definitive dermis. These findings suggest that a parallel array of subectodermal FB1-positive fibers constitutes a precocious fiber system in the presumptive dermis prior to the substantial formation of collagenous fibers. These fibers could be developmentally linked to oxytalan fibers, which are known to be present in the papillary dermis in mature cutaneous tissue.

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Ultrastructural Studies on the Lymphatic Anchoring Filaments

Cited by 353 publications

References 18 publications

Late Deposition of Elastin to Vertical Microfibrillar Fibers in the Presumptive Dermis of the Chick Embryonic Tarsometatarsus

Late Deposition of Elastin to Vertical Microfibrillar Fibers in the Presumptive Dermis of the Chick Embryonic Tarsometatarsus

Cell adhesion and integrin binding to recombinant human fibrillin‐1

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

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