Ultrastructural studies on the intercellular bridges between the oocyte and follicle cells in the lizard Acanthodactylus scutellatus hardyi

Bou-Resli, M. N.

doi:10.1007/bf00519868

Cited by 24 publications

(11 citation statements)

References 56 publications

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“…The structure and development of follicles in S. mitranus has been reconstructed as shown in the Figure . In general, the structure of the germinal beds and some cytoplasmic and nuclear features of oogonia and early oocytes in S. mitranus are similar to those described for other lizards (Arronet, ; Bou‐Resli, ; Filosa & Taddei, ; Hubert, , , ; Hubert, ; Hubert, ; Klosterman, ; Klosterman, ). However, in L. sicula , the presence of cytoplasmic bridges has been reported between the developing oogonia in the germinal bed (Filosa & Taddei, ).…”

Section: Discussionsupporting

confidence: 75%

“…Hubert () proposed a model for granulosa cell differentiation in L. vivipara , which was later applied to other lizard species (Hubert, ; Klosterman, ). In contrast to Bou‐Resli () who attributed mitotic activity to small and intermediate cells, most reports suggest that this is not the case and only small cells are observed in mitosis (Betz, ; Hubert, ; Hubert, ). In fact, it has been shown by measuring DNA content in the granulosa cells in follicles at different stages in the two species L. sicula and G. coeruleus that DNA was synthesized only in the small cells (Klosterman, ; Olmo & Taddei, ).…”

Section: Discussionmentioning

confidence: 88%

“…However, in L. sicula , the presence of cytoplasmic bridges has been reported between the developing oogonia in the germinal bed (Filosa & Taddei, ). Such bridges were not detected in S. mitranus or other species such as A. scutellatus (Bou‐Resli, ) and G. coeruleus (Klosterman, ). The paranucleolar mass is thought to be an important cytological marker of primordial germ cells and oogonia in lizards (Hubert, , , , , ), but was not detected in the oogonia of S. mitranus as in other lizards (Filosa & Taddei, ; Klosterman, ; Klosterman, ).…”

Section: Discussionmentioning

confidence: 99%

“…The ultrastructure of oogenesis of lizards has been described in few species, including Zootoca vivipara (Hubert, ), Lacerta sicula (Filosa & Taddei, ), Acanthodactylus scutellatus (Bou‐Resli, ), and Elgaria coerulea (Klosterman, ). Notably, relatively few species of squamates have been studied (Vieira et al, ), including the family Scincidae, the largest family of lizards.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural and histochemical study of previtellogenic oogenesis in the desert lizard Scincus mitranus (Squamata, Sauropsida)

Alwasel

Harrath

2019

Journal of Morphology

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The structure of the granulosa in reptilian sauropsids varies between groups. We investigated the follicle development in the desert lizard Scincus mitranus. In the germinal bed, oogonia, and primary oocytes were identified and found to be interspersed between the epithelial cells. Previtellogenesis was divided into three stages: early, transitional, and late previtellogenic stages. During the early previtellogenic stage (diplotene), the oocyte is invested by small epithelia cells that formed a complete single layer, which may be considered as a young follicle. The transitional previtellogenic stage was marked by proliferation and differentiation of the granulosa layer from a homogenous layer consisting of only small cells to a heterogeneous layer containing three cell types: small, intermediate, and large cells. The late previtellogenic stage was marked by high‐synthetic activity of large cells and the initiation of cytoplasmic bridges between large granulosa cells and the oocyte. Small cells were the only type of granulosa cells that underwent division. Thus, these cells may be stem cells for the granulosa cell population and may develop into intermediate and subsequently large cells. The intermediate cells may be precursors of large cells, as suggested by their ultrastructure. The ultrastructure of the large granulosa was indicative of their high synthetic activity. Histochemical analysis indicated the presence of cholesterol and phospholipids in the cytoplasm of large cells, the zona pellucida, among the microvilli, in the bridges region, and in the cortical region of the oocyte cytoplasm. These materials may be transferred from large cells into the oocyte through cytoplasmic bridges and provide nutritive function to large cells rather than functioning in steroidogenesis or vitellogenesis.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrastructural and histochemical study of previtellogenic oogenesis in the desert lizard Scincus mitranus (Squamata, Sauropsida)

Alwasel

Harrath

2019

Journal of Morphology

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“…This layer contains small follicle cells, intermediate cells and large "pyriform cells" so called because of their shape. The pyriform cells represent, in the vertebrates studied so far, a unique case of communication between follicle cells and oocyte through intercellular bridges (Ghiara et al 1968;Neaves 1971;Taddei 1972;Bou-Resly 1974;Hubert 1976). Furthermore, in Podarcis sicula, these peculiar follicle cells differentiate from small follicle cells through intermediate cells (Filosa et al 1979), the heterologous fusion of somatic follicle cells with the oocyte preceding their differentiation into intermediate and pyriform cells (Andreuccetti et al 1978).…”

Section: Introductionmentioning

confidence: 98%

Immunolocalization of cytoskeletal proteins in the previtellogenic ovarian follicle of the lizard Podarcis sicula

Maurizii

Taddei

1996

Cell and Tissue Research

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We report the immunolocalization of intermediate filament proteins (vimentin and cytokeratins) in the previtellogenic ovarian follicle of the lizard Podarcis sicula. Vimentin is present, in accordance with their mesenchymal origin, in all the cells of the polymorphic follicular epithelium (small, intermediate and pyriform). Cytokeratin, absent from the small follicle cells, is present in those cells (intermediate and pyriform) connected to the oocyte by an intercellular bridge. In particular, this protein surrounds the nucleus in the pyriform cells and is mainly localized at the apex adjacent to the oocyte surface; it forms a network, that crosses the zona pellucida through the intercellular bridge and appears to be continuous with a cortical ring of cytokeratin in the oocyte. The presence of a cytokeratin cytoskeleton in the pyriform cells in continuity with that of the oocyte lends support to the previously reported hypothesis that, during oogenesis in Podarcis sicula, somatic cells constitute an integral system with the germ cell and provide for its growth. Preliminary observations indicating the presence of actin and tubulin inside the cytoplasmic bridges connecting the pyriform cells to the oocyte are in agreement with an involvement of these connections in the transfer of cytoplasmic materials.

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Cytokeratin cytoskeleton in the differentiating ovarian follicle of the lizardPodarcis sicula Raf

Maurizii¹,

Saverino²,

Taddei³

1997

Mol. Reprod. Dev.

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Ultrastructural studies on the intercellular bridges between the oocyte and follicle cells in the lizard Acanthodactylus scutellatus hardyi

Cited by 24 publications

References 56 publications

Ultrastructural and histochemical study of previtellogenic oogenesis in the desert lizard Scincus mitranus (Squamata, Sauropsida)

Ultrastructural and histochemical study of previtellogenic oogenesis in the desert lizard Scincus mitranus (Squamata, Sauropsida)

Immunolocalization of cytoskeletal proteins in the previtellogenic ovarian follicle of the lizard Podarcis sicula

Cytokeratin cytoskeleton in the differentiating ovarian follicle of the lizardPodarcis sicula Raf

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