Ultrastructural Studies of the Human Round Window Membrane

Carpenter, Anna‐Mary; Muchow, David C.; Goycoolea, Marcos V.

doi:10.1001/archotol.1989.01860290043012

Cited by 53 publications

(39 citation statements)

References 16 publications

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“…Although the round window membrane in humans is much thicker than that in a guinea pig, a water-tight seal is achieved similarly by a single layer of cells: the outer epithelium [Goycoolea and Lundman, 1997;Carpenter et al, 1989;Cureoglu et al, 2005]. The membrane morphology changes due to otitis media [Sahni et al, 1987] are similar to the observations in guinea pig OME models [Cureoglu et al, 2005].…”

Section: Change In Cochlear Response In Ome Earsmentioning

confidence: 58%

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Dai

Gan²

2009

Audiol Neurotol

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Our previous studies confirm that middle ear mobility is reduced in the presence of otitis media with effusion (OME). Variations in middle ear function may result in changes in cochlear response in OME ears. With the long-term goal of evaluating cochlear function in OME ears, the aim of this study was to measure the displacement of the basilar membrane (BM) in guinea pig ears with OME. Vibrations of the BM at the apex and basal turn were measured in an in vitro preparation extracted 3 and 14 days after injection of lipopolysaccharide in the middle ear of guinea pigs. The results show that the displacement sensitivity of the BM at the apex and the basal turn to sound pressure in the ear canal was reduced up to 25 dB at their characteristic frequencies, respectively. Cochlear gain with respect to umbo movement was also changed in ears with OME in both groups. This study provides data for analysis of the change of BM vibration in a guinea pig OME model.

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Section: Change In Cochlear Response In Ome Earsmentioning

confidence: 58%

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Dai

Gan²

2009

Audiol Neurotol

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“…RWMs of the human, guinea pig and chinchilla have comparable ultrastructures of three basic layers: an outer epithelial layer facing the middle ear, a middle fibrous layer, and an inner epithelium (Carpenter et al, 1989;Goycoolea and Lundman, 1997). Interspecies variations are mainly in thickness, being thinnest in chinchillas and thickest in humans.…”

Section: Comparison With Human and Guinea Pig Rwm -Control Casementioning

confidence: 99%

Complex modulus of round window membrane over auditory frequencies in normal and otitis media chinchilla ears

Wang

Nakmali

Gan

2015

IJECB

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Abstract:To better reveal the mechanical properties of round window membrane (RWM) in normal and pathological ears, the complex modulus of chinchilla RWM was determined by measuring its dynamic behaviour together with the finite element simulation. The acute otitis media (AOM) was created by transbullar injection of Haemophilus influenzae in chinchillas and RWM specimens in AOM ears were prepared four days post inoculation. Vibration of the RWM induced by coil-magnet force stimulation was measured by laser Doppler vibrometry over frequencies of 0.2-8 kHz. A finite element model-based inverse-problem solving method was used to determine the complex modulus of each RWM specimen in the frequency domain. Experimental results of the AOM ears indicated that the resonance frequency decreased by 13.94% compared with the normal ears and the mean storage modulus and loss modulus were decreased by 65% and 32%, respectively. Keywords: round window membrane; RWM; complex modulus; viscoelasticity; otitis media; finite element model.Reference to this paper should be made as follows: Wang, X., Nakmali, D. and Gan, R.Z. (2015) 'Complex modulus of round window membrane over auditory frequencies in normal and otitis media chinchilla ears', Int. J.

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“…Histologically, the RWM consists of three basic layers: outer epithelial layer, middle connective tissue layer, and inner mesothelial layer (1,5,6). This triple-layered RWM, called secondary tympanic membrane (TM) is very similar to that of the TM.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Characteristics of the Round Window Membrane During Pneumococcal Otitis Media in Rat

Yoon

Hellström

2002

J Korean Med Sci

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To understand better the pathogenesis of inner ear (IE) damage caused by otitis media (OM), the round window membrane(RWM) structure was investigated in a rat model for pneumococcal otitis media (POM). The RWM of 25 rats were evaluated light and electron microscopically on 1 day, 3 days, 6 days, 10 days, and 20 days after the unilateral inoculation of type 3 pneumococcus suspension into their middle ear cavities. The thickness of the RWM increased in various stages of the pneumococcus-evoked otitis media, compared with that of the normal. The thickening was most pronounced on day 1, being about 4 to 5 times greater than that of the normal RWM. All layers of the RWM were affected by the pneumococcal infection, but the major changes were confined to the subepithelial space close to the basement membrane (BM). Together with alterations to the BM, the most distinct pathological features were characterized by an increase and hypertrophy of fibroblasts in association with abundant collagen fibers. Elastic fibers observed close to the inner mesothelial layer under a high power magnification also increased during the experiment. These results will be relevant to a better understanding of the histologic implication of RWM in stages of acute otitis media involving pneumococcus-evoked otitis media.

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Ultrastructural Studies of the Human Round Window Membrane

Cited by 53 publications

References 16 publications

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Complex modulus of round window membrane over auditory frequencies in normal and otitis media chinchilla ears

Ultrastructural Characteristics of the Round Window Membrane During Pneumococcal Otitis Media in Rat

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