Ultrastructural relationships of spinal primary afferent fibres with neuronal and non-neuronal cells in the myenteric plexus of the cat oesophago-gastric junction

Mazzia, Christophe; Clerc, Nadine

doi:10.1016/s0306-4522(97)00058-4

Cited by 38 publications

(28 citation statements)

References 37 publications

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“…Scale bar 1 µm bers in the large NFBs. This is similar to B-HRP-labeled spinal primary afferent fibers in the myenteric plexus area of the cat LES (see Mazzia and Clerc 1997) and to unmyelinated group IV somatic fibers (Heppelmann et al 1994). The absence of gap junctions excludes any intercommunication between the muscle cells and the primary afferent fibers in this gut area.…”

Section: Discussionsupporting

confidence: 73%

“…The labeled primary afferent fibers exhibited different reaction product appearances according to the protocol with which they were revealed (Mazzia and Clerc 1997). The TMB/AHM reaction product appeared as needlelike crystals obscuring the cytoplasmic organelles but not the axolemma.…”

Section: Resultsmentioning

confidence: 99%

“…In the case of the spinal primary afferent fibers in the cat LES, this hypothesis is strengthened by a previous electron microscope study showing that, in the myenteric plexus area, these fibers exhibit bare areas of axolemma that might be sites of mechanical transduction (Mazzia and Clerc 1997). However, no ultrastructural examination has been made of the afferent fibers within the muscle coat.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural analysis of spinal primary afferent fibers within the circular muscle of the cat lower esophageal sphincter

Mazzia

Clerc²

2000

Histochemistry and Cell Biology

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We have analyzed the ultrastructural characteristics and environment of spinal primary afferent fibers that run within the circular muscle of the cat lower esophageal sphincter. These were selectively labeled by anterogradely transported cholera toxin B subunit conjugated with horseradish peroxidase. Most of the labeled fibers were perpendicular to the muscle cells but some ran sinuously or parallel to the muscle cells. All the labeled fibers were unmyelinated and exhibited relatively rare varicosities. Most of the fibers were in large nerve fiber bundles surrounded by perineurium and probably project to the mucosa. Only some fibers that were in small nerve fiber bundles with no perineurium ran parallel to the musculature and established close relationships with smooth muscle cells. They might be a small subpopulation of the spinal tension receptors, most of the other spinal tension receptors being located in the myenteric plexus area, between the circular and longitudinal muscle.

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Section: Discussionsupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrastructural analysis of spinal primary afferent fibers within the circular muscle of the cat lower esophageal sphincter

Mazzia

Clerc²

2000

Histochemistry and Cell Biology

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“…This enables the autonomic nervous system to establish`pre-central' re¯ex arcs independent of the central nervous system. Such pre-central re¯exes are supposed to play important regulatory roles in the gastrointestinal tract (Furness and Costa, 1987;Grider and Jin, 1994;Szurszewski and Miller, 1994;Mazzia and Clerc, 1997), genitourinary tract (Keast and Chiam, 1994;Sann, 1998), bronchopulmonary system (Baluk and Gabella, 1989;Myers, Undem and Kummer, 1996) and heart (Steele et al, 1994;Hardwick, Mawe and Parsons, 1995). To further elucidate the neuronal network that might be involved in the control of ocular homeostasis, e.g.…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Neurons in the Duck Choroid are Contacted by CGRP-Immunoreactive Nerve Fibres: Evidence for a Local Pre-central Reflex Arc in the Eye

Schrödl

Schweigert

Brehmer

et al. 2001

Experimental Eye Research

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“…Esophageal neuromuscular junctions receive a dual innervation from both vagal nerve fibers originating in the brain stem and from varicose enteric nerve fibers originating in the myenteric plexus, the so-called enteric co-innervation [for review see 21, 34]. Enteric neurons in the esophagus are contacted by primary afferents of spinal and vagal origin [19,20,25]. A substantial number of these primary afferents, mainly of spinal origin, were shown to be immunoreactive to the transient receptor potential ion channel of the vanilloid type 1 (TRPV1) and called capsaicin-sensitive sensory neurons [12,14,15,23,30,31,33].…”

mentioning

confidence: 99%

Key Role of Mucosal Primary Afferents in Mediating the Inhibitory Influence of Capsaicin on Vagally Mediated Contractions in the Mouse Esophagus

Boudaka

Wörl

Shiina

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Transient receptor potential ion channel of the vanilloid type 1 (TRPV1)-dependent pathway, consisting of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons, was suggested to mediate the inhibitory effect of capsaicin on the vagally mediated striated muscle contractions in the rat esophagus. These primary afferent neurons upon entering into the esophagus are distributed through the myenteric plexus, terminating either in the myenteric ganglia or en route to the mucosa where they branch into a delicate net of fine varicose fibers. Therefore, this study aimed to investigate whether the mucosal primary afferents are a main mediator for the capsaicin inhibitory influence on vagally mediated contractions in the mouse esophagus. For this purpose, the vagally induced contractile activity of a thoracic esophageal segment was measured in the circular direction with a force transducer. Vagal stimulation (30 µsec, 25 V, 1-50 Hz for 1 sec) produced monophasic contractile responses, whose amplitudes were frequency-dependent. These contractions were completely abolished by d-tubocurarine (5 µM) while resistant to atropine (1 µM) and hexamethonium (100 µM). Capsaicin (30 µM) significantly inhibited the vagally induced contractions in esophagi with intact mucosa while its effect on preparations without mucosa was insignificant. Additionally, immunocytochemistry revealed the presence of TRPV1-positive nerve fibers in the tunica mucosa. Taken together, we conclude that in the mouse esophagus, capsaicin inhibits the vagally mediated striated muscle contractions mainly through its action on mucosal primary afferents, which in turn activate the presumed inhibitory local reflex arc. KEY WORDS: capsaicin-sensitive neurons, enteric co-innervation, local effector function, TRPV1, vagus nerve.J. Vet. Med. Sci. 69(4): 365-372, 2007 A peripheral reflex arc composed of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons have been shown to negatively influence the vagally mediated striated muscle contractions in the rat esophagus [27]. Esophageal neuromuscular junctions receive a dual innervation from both vagal nerve fibers originating in the brain stem and from varicose enteric nerve fibers originating in the myenteric plexus, the so-called enteric co-innervation [for review see 21, 34]. Enteric neurons in the esophagus are contacted by primary afferents of spinal and vagal origin [19,20,25]. A substantial number of these primary afferents, mainly of spinal origin, were shown to be immunoreactive to the transient receptor potential ion channel of the vanilloid type 1 (TRPV1) and called capsaicin-sensitive sensory neurons [12,14,15,23,30,31,33]. These neurons can modulate intestinal motility by transferring signals from the gastrointestinal tract to the central nervous system and simultaneously releasing transmitters, in particular substance P (SP) and calcitonin generelated peptide (CGRP) that can influence the activity of intrinsic neurons [14,16].Primary affe...

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Ultrastructural relationships of spinal primary afferent fibres with neuronal and non-neuronal cells in the myenteric plexus of the cat oesophago-gastric junction

Cited by 38 publications

References 37 publications

Ultrastructural analysis of spinal primary afferent fibers within the circular muscle of the cat lower esophageal sphincter

Ultrastructural analysis of spinal primary afferent fibers within the circular muscle of the cat lower esophageal sphincter

Intrinsic Neurons in the Duck Choroid are Contacted by CGRP-Immunoreactive Nerve Fibres: Evidence for a Local Pre-central Reflex Arc in the Eye

Key Role of Mucosal Primary Afferents in Mediating the Inhibitory Influence of Capsaicin on Vagally Mediated Contractions in the Mouse Esophagus

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