Ultrastructural pathology of the sperm flagellum: association between flagellar pathology and fertility prognosis in severely asthenozoospermic men

Chemes, H; Olmedo, S. Brugo; Carrere, C.; Osés, R.; Carizza, C.; Leisner, M.; Blaquier, Jörge A.

doi:10.1093/humrep/13.9.2521

Cited by 140 publications

(29 citation statements)

References 13 publications

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“…The diameter of the human sperm tail varies from 400 to 880 nm, according to the literature (Chemes and Rawe, 2003; Chemes et al, 1998; Serres et al, 1983). This wide range of reported values is likely due to the differences in sample preparation methods, such as sample dehydration, required for electron microscopy, which often distorts the original sample size.…”

Section: Resultsmentioning

confidence: 99%

Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation

et al. 2018

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SUMMARY Ion channels control sperm navigation within the female reproductive tract and, thus, are critical for their ability to find and fertilize an egg. The flagellar calcium channel CatSper controls sperm hyperactivated motility and is dependent on an alkaline cytoplasmic pH. The latter is accomplished by either proton transporters or, in human sperm, via the voltage-gated proton channel Hv1. To provide concerted regulation, ion channels and their regulatory proteins must be compartmentalized. Here, we describe flagellar regulatory nanodomains comprised of Hv1, CatSper, and its regulatory protein ABHD2. Super-resolution microscopy revealed that Hv1 is distributed asymmetrically within bilateral longitudinal lines and that inhibition of this channel leads to a decrease in sperm rotation along the long axis. We suggest that specific distribution of flagellar nanodomains provides a structural basis for the selective activation of CatSper and subsequent flagellar rotation. The latter, together with hyperactivated motility, enhances the fertility of sperm.

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Section: Resultsmentioning

confidence: 99%

Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation

et al. 2018

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“…The genotypic sperm defect DFS (dysplasia of the fibrous sheath) (Chemes et al. , 1987, 1998), associated with respiratory pathology and familial inheritance, is characterized by different distortions in the fibrous sheath, microtubular doublets and mitochondrial sheath, all arranged in a short and thick tail.…”

Section: Mitochondrial Energy Metabolism In Human Spermmentioning

confidence: 99%

The role of mitochondria in energy production for human sperm motility

et al. 2011

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Mitochondria of spermatozoa are different from the corresponding organelles of somatic cells, in both their morphology and biochemistry. The biochemical differences are essentially related to the existence of specific enzyme isoforms, which are characterized by peculiar kinetic and regulatory properties. As mitochondrial energy metabolism is a key factor supporting several sperm functions, these organelles host critical metabolic pathways during germ cell development and fertilization. Furthermore, spermatozoa can use different substrates, and therefore activate different metabolic pathways, depending on the available substrates and the physico-chemical conditions in which they operate. This versatility is critical to ensure fertilization success. However, the most valuable aspect of mitochondria function in all types of cells is the production of chemical energy in the form of ATP which can be used, in the case of spermatozoa, for sustaining sperm motility. The latter, on the other hand, represents one of the major determinants of male fertility. Accordingly, the presence of structural and functional alterations in mitochondria from asthenozoospermic subjects confirms the important role played by these organelles in energy maintenance of sperm motility. The present study gives an overview of the current knowledge on the energy-producing metabolic pathways operating inside human sperm mitochondria and critically analyse the differences with respect to somatic mitochondria. Such a comparison has also been carried out between the functional characteristics of human sperm mitochondria and those of other mammalian species. A deeper understanding of mitochondrial energy metabolism could open up new avenues of investigation in bioenergetics of human sperm mitochondria, both in physiological and pathological conditions.

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“…Asthenozoospermia (AS), defined as low-motile spermatozoa in the ejaculate, is a frequent cause of human male infertility (Chemes et al 1998). It is reported that more than 80% of the spermatozoa from infertile patients had motility defects and 19% of them were diagnosed with AS (Curi et al 2003).…”

Section: Introductionmentioning

confidence: 99%

PARK7 protein translocating into spermatozoa mitochondria in Chinese asthenozoospermia

Sun¹,

Zhang²,

Zhao³

et al. 2014

Reproduction

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PARK7 (DJ1) is a multifunctional oxidative stress response protein that protects cells against reactive oxygen species (ROS) and mitochondrial damage. PARK7 defects are known to cause various physiological dysfunctions, including infertility. Asthenozoospermia (AS), i.e. low-motile spermatozoa in the ejaculate, is a common cause of human male infertility. In this study, we found that downregulation of PARK7 resulted in increased levels of lipid peroxide and ROS, decreased mitochondrial membrane potential, and reduced mitochondrial complex I enzyme activity in the spermatozoa from AS patients. Furthermore, it was observed that PARK7 was translocated into the mitochondria of damaged spermatozoa in AS. Finally, we examined the oxidative state of PARK7 and the results demonstrated the enhancement of oxidation, expressed by increased sulfonic acid residues, the highest form of oxidation, as the sperm motility decreased. Taken together, these results revealed that PARK7 deficiency may increase the oxidative stress damage to spermatozoa. Our present findings open new avenues of therapeutic intervention targeting PARK7 for the treatment of AS.

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Ultrastructural pathology of the sperm flagellum: association between flagellar pathology and fertility prognosis in severely asthenozoospermic men

Cited by 140 publications

References 13 publications

Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation

Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation

The role of mitochondria in energy production for human sperm motility

PARK7 protein translocating into spermatozoa mitochondria in Chinese asthenozoospermia

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