Ultrastructural Pathology of the Sperm Flagellum

Chemes, H; Olmedo, S. Brugo; Carrere, C.; Osés, R.; Carizza, C.; Leisner, M.; Blaquier, Jörge A.

doi:10.1097/00005392-199905000-00116

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional aberrations have included absence of the radial spokes [32], peripheral microtubule defects, dysplasia for the fibrous sheath [12], non-specific axoneme defects [33], complete ciliary aplasia, orientation defects [34], and absence of the central microtubule pair [28,31,35]. Wolff et al also reported a case of immotile cilia syndrome where the axonemes of the spermatozoa tails were complete except they lacked the two central microtubules.…”

Section: Discussionmentioning

confidence: 99%

“…In one of the few papers to look at more than just one or a few patients; 247 severely asthenozoospermic patients where assessed for ultrastructural defects. The ultrastructural studies showed two main alterations: 83% had non-specific flagellar anomalies (NSFA), affecting variable numbers of spermatozoa; 17% had dysplasia of the fibrous sheath which affected between 70% and 100% of the spermatozoa in several cases [12]. Yokota et al showed defects in the dynein arms, central microtubules, and total axoneme defects in one report [39] and still another report showed disorganization of mitochondria in the mid-piece's capsule and irregular arrangement of the axoneme's thick fibers in addition to two to four axonemes surrounded by the same cellular structure was also seen by other investigators [40].…”

Section: Discussionmentioning

confidence: 99%

“…Successful treatment of PCD with the birth of healthy babies following embryo transfer on day three or earlier has been reported using sub-zonal insemination (SUZI) [4][5][6] and intracytoplasmic sperm injection (ICSI) [7][8][9][10][11][12][13]. The hypo-osmotic swelling (HOS) test [14] was used in conjunction with ICSI of immotile spermatozoa with success in several cases as well [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture. Materials and methods Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage. Results 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks. Conclusions The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Kordus

Price

Davis

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…On the other hand, asthenozoospermia is a very frequent cause of male infertility. Previous reports demonstrated that ultrastructural abnormality of spermatozoa is observed in asthenozoospermic or teratozoospermic sterile men (26,27). A high incidence of flagellar pathology was found to be the underlying cause of motility disorders in severely asthenozoospermic patients (28).…”

Section: Kisimo Locus Required For Spermatogenesis 14793mentioning

confidence: 92%

Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis

Yanaka

Kobayashi

Wakimoto³

et al. 2000

Journal of Biological Chemistry

View full text Add to dashboard Cite

Spermatogenesis is a developmental process that occurs in several phases and is regulated by a large number of gene products. An insertional transgenic mouse mutant (termed kisimo mouse) has been isolated that results in abnormal germ-cell development, showing abnormal elongated spermatids in the lumina of seminiferous tubules. We cloned the disrupted locus of kisimo and identified a novel testis-specific gene, THEG, which is specifically expressed in spermatids and was disrupted in the transgenic mouse. The yeast two-hybrid screening method revealed that THEG protein strongly interacts with chaperonin containing t-complex polypeptide-1⑀, suggesting that THEG protein functions as a regulatory factor in protein assembly. Our findings indicate that the kisimo locus is essential for the maintenance of spermiogenesis and that a gene expression disorder may be involved in male infertility.The integration of foreign DNA into the mouse germ line by retroviral infection or microinjection can result in insertional disruption of endogenous genes with important roles in development (1-3). Foreign DNA insertion provides an approach to the cloning of disrupted host loci. By using the introduced DNA as a probe to screen genomic libraries from mutant animals, it has been possible in a few instances to isolate clones that contain DNA flanking the exogenous integrated material and, thus, include portions of the interrupted gene (4, 5). We have produced a series of 12 transgenic mouse lines with the human phosphodiesterase 5A (PDE5A) 1 gene (6). As the mice were bred, it became evident that many males of one line were sterile and that the sterility arose from a defect in spermatogenesis (we named this mutant kisimo for a Japanese goddess of easy delivery). Because the sterility segregated with the hemizygous transgene and occurred in the absence of the detectable expression of the transgene, we concluded that the abnormal phenotype was due to mutagenesis by insertion of the transgene. In this study, to clone the junctions between the inserted transgene and adjoining cellular DNA, we used thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) (7) and defined a genomic locus important for spermatogenesis. The process of spermatogenesis in the mouse has been well characterized at the morphological level. The spermatogenic process can be subdivided into three main phases. Spermatogonia, the germinal stem cells, undergo mitosis to produce additional spermatogonia, a portion of which develop into primary spermatocytes. The spermatocytes enter meiosis and proceed through two cell divisions to give rise to haploid round spermatids. These, in turn, undergo a complex morphological transformation involving nuclear condensation and elongation resulting in the production of mature spermatozoa. However, at the molecular level, relatively little is known about the control of cellular differentiation and the architectural changes during spermatogenesis.In this study, we found that an insertion of foreign DNA results in abnormal male ...

show abstract

Ultrastructural Pathology of the Sperm Flagellum

Cited by 2 publications

References 0 publications

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis

Contact Info

Product

Resources

About