Ultrastructural Pathology of the Sperm Flagellum: Association Between Flagellar Pathology and Fertility Prognosis in Severely Asthenozoospermic Men

Chemes, H; Olmedo, S. Brugo; Carrere, C.; Osés, R.; Carizza, C.; Leisner, M.; Blaquier, Jörge A.

doi:10.1016/s0022-5347(05)69031-3

Cited by 54 publications

(93 citation statements)

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“…More particularly, they share in the organization of 9+2 of the axonemal microtubule in sperm tail [24]. Chemes et al(1998) in an ultra structural study on asthenozoospermic patients with dysplasia of fibrous sheat (DFS) showed missing axonemal central pairs and complete distortion of 9+2 axonemal structure in many of patients, that not apparent on semen smears [25]. In this regard, reduction of TUBB2B might lead to the formation of defective microtubules of sperm flagellum and, consequently, result in defective sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…Petersen et al (1999) demonstrated that the absence of one or more outer dense fiber proteins could affect sperm motility and cause nonfunctional tails; thus, outer dense fiber proteins may act as markers of male factor infertility [28]. An ultra structural study of asthenozoospermia men with DFS by Chemes et al(1998) showed the abnormal extension of ODF to the principal piece [25]. The present study demonstrated that ODF2 was less expressed in asthenozoospermic patients that might result in abnormality of outer dense fibers and decreased elasticity of sperm flagellum which could affect sperm motility.…”

Section: Discussionmentioning

confidence: 99%

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A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Hashemitabar

Sabbagh

Orazizadeh

et al. 2015

J Assist Reprod Genet

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Purpose Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients. Methods Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/ decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/ time-of-flight (MALDI-TOF-TOF) mass spectrometry. Results Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p<0.05) were excised and exploited using MALDI-TOF-TOF mass spectrometry. They led to the identification of the following 14 unique proteins: Tubulin beta 2B; glutathione S-transferase Mu 3; keratin, type II cytoskeletal 1; outer dense fiber protein 2; voltage-dependent anionselective channel protein 2; A-kinase anchor protein 4; cytochrome c oxidase subunit 6B; sperm protein associated with the nucleus on the X chromosome B; phospholipid hydroperoxide glutathione peroxidase-mitochondrial; isoaspartyl peptidase/L-asparaginase; heat shock-related 70 kDa protein 2; stress-70 protein, mitochondrial; glyceraldehyde-3-phosphate dehydrogenase, testis-specific and clusterin. Conclusion Fourteen proteins present in different amounts in asthenozoospermic sperm tail samples were identified, four of which are reported here for the first time. These proteins might be used as markers for the better diagnosis of sperm dysfunctions, targets for male contraceptive development, and to predict embryo quality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Hashemitabar

Sabbagh

Orazizadeh

et al. 2015

J Assist Reprod Genet

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“…Chemes et al [87] proposed the term dysplasia of the fibrous sheath (DFS) for the disorder, which is also known as stump tail syndrome and short-tail spermatozoa. Spermatozoa have substantially reduced, if any, motility due to fibrous for DSF [88].…”

Section: Dysplasia Of the Fibrous Sheath Of The Tailmentioning

confidence: 99%

Ultrastructure of Spermatozoa from Infertility Patients

Брагина¹,

Bocharova²

2018

Spermatozoa - Facts and Perspectives

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Standard examination of human semen currently remains a main test for examination of male fertility disorders. Although parameters of sperm quality in fertile men are generally higher than in sterile ones, there is a substantial overlap between the two populations, indicating that other important factors affect fertility, but are not assessed in conventional assay. Currently, tests determining the functional properties of sperm have been intensively developed. This review considers an electron microscopic examination of sperm, which assesses the structure and function of the sperm nuclear, penetration and motor apparatus. The detection of sperm chromatin structure can help to understand the causes of early embryonic malformation. Genetically caused and functional disorders of the structure and function of spermatozoa are discussed. Indications for electron microscopic examination of spermatozoa in fertility disorders are given.

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“…First introduced by Chemes et al, [1] the denomination Dysplasia of the Fibrous Sheath (DFS) (including 'stump tail' and 'short tail' sperm defects) refers to a condition of absent or severely reduced sperm motility associated to major alterations in the fibrous sheath and dysplastic development of the tail during spermatogenesis [1,2]. At light microscopy this isolate defect, involving the majority of ejaculated sperm, show short and irregularly thick tails.…”

Section: Introductionmentioning

confidence: 99%

“…At light microscopy this isolate defect, involving the majority of ejaculated sperm, show short and irregularly thick tails. Ultrastructural studies revealed a heterogeneous array of sperm tail anomalies caused by a hyperplastic and disorganized fibrous sheath and the alteration of other cytoskeletal and axonemal components of the flagellar [2][3][4]. Frequent familial incidence of DFS has been reported that suggests a genetic origin of this syndrome [2,3,5].…”

Section: Introductionmentioning

confidence: 99%

Meiotic segregation and sperm DNA fragmentation in Tunisian men with dysplasia of the fibrous sheath (DFS) associated with head abnormalities

Ghédir

Mehri

Mogharrabi

et al. 2014

J Assist Reprod Genet

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Purpose Dysplasia of the Fibrous Sheath (DFS) is a primitive flagellar pathology for which a broad spectrum of ultrastructural flagellar abnormalities has been described responsible for a severe to total asthenozoospermia. To this phenotype other morphological abnormalities including cephalic and abnormalities in nuclear structure can be associated that could compromise embryonic development in case of use of Assisted Reproductive Technology (ART). The aim of this study was to evaluate the level of DNA fragmentation and aneuploidy rate in ejaculated spermatozoa of Tunisian men presented with DFS sperm defect associated to high percentage of head abnormalities and to compare the results with those from fertile men. Methods Sperm DNA fragmentation was evaluated by the terminal desoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick-end labelling (TUNEL) assay. The study of meiotic segregation was performed by Fluorescence in situ hybridization (FISH) for chromosomes X, Y and 18. Results The mean DNA fragmentation index was significantly higher in patients compared to the control group. FISH revealed a significantly higher incidence of sperm aneuploidies compared with controls. All patients showed elevated frequencies of sex chromosomes disomy, disomy 18 and diploidy. Conclusions In some cases of syndromic teratozoospermia due to sperm tail structural abnormalities, such as DFS, other morphological cephalic abnormalities may be associated. In these cases we have demonstrated impaired sperm nuclear quality which will affect the results in ICSI. Hence the interest of a thorough study of the sperm nucleus in these forms of infertility in order to predict the chances of success in ART.

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Ultrastructural Pathology of the Sperm Flagellum: Association Between Flagellar Pathology and Fertility Prognosis in Severely Asthenozoospermic Men

Cited by 54 publications

References 0 publications

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Ultrastructure of Spermatozoa from Infertility Patients

Meiotic segregation and sperm DNA fragmentation in Tunisian men with dysplasia of the fibrous sheath (DFS) associated with head abnormalities

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