Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads

Prisant, Nadia; Escalier, Denise; Jc, Soufir; Morillon, Maurice; Schöevaërt, Damien; Misrahi, Micheline; Tachdjian, Gérard

doi:10.1093/humrep/del481

Cited by 36 publications

(31 citation statements)

References 45 publications

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“…In addition to reduced testis weight and sperm density, F 1 hybrid males from M. m. musculus PWD mothers presented abnormal sperm types similar to those previously connected with sterility. Abnormal sperm head shapes often arise as byproducts of aneuploidy (Prisant et al 2007;Perrin et al 2008;Revay et al 2009). In rodents, alterations of the sperm hook are correlated with decreases in fertilization success (Immler et al 2007;Firman and Simmons 2009), and severely amorphous sperm heads are inefficient at penetrating the ova (Krzanowska and Lorenc 1983;Oka et al 2007;Styrna 2008).…”

Section: Discussionmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F 1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F 2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F 1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice.

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Section: Discussionmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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“…The increased sperm head length results from an abnormally elongated nucleus, which also presents particular membranous layers between the outer and inner leaves of the nuclear envelope. The sperm nuclear anomalies are also associated with anomalies of the neck region, persistence of cytoplasmic residual material and an increased frequency of chromosomal aneuploidies, together with impaired chromatin compaction, possibly because of meiotic non-disjunction during spermatogenesis [63]. Low ICSI fertilization rates have also been found in men with severely elongated sper ma tozoa compared with other sperm morphology abnormalities [64].…”

Section: Elongated Spermatozoamentioning

confidence: 99%

Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen

Menkveld¹

2010

Asian J Androl

169

109

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The very low cut-off value for sperm morphology of 4% morphologically normal spermatozoa, as proposed in the new edition of the World Health Organization (WHO) manual on semen analysis, is in agreement with recently published values and reflects the trend of a decline in reported mean values for normal sperm morphology. The reduced value for morphologically normal spermatozoa over the years may be due to several factors. The first is the introduction of strict criteria for the evaluation of sperm morphology. Other reasons may include the introduction of additional criteria for sperm morphology abnormalities and the suggested decrease in semen parameters because of increasing negative environmental influences. Although on its own the newly proposed very low normal value may not provide the strong predictive value for a males' fertility potential, as originally reported for sperm morphology evaluated according to strict criteria, a good predictive value can still be obtained if the holistic, strict approach for sperm morphology evaluation is followed together with additional sperm morphology parameters now available, because certain morphology patterns and sperm abnormalities are now known to be of strong prognostic value. In addition, better international standardization of the technical methodology, consensus on the interpretation of sperm morphology evaluation criteria and standardized international external quality control (EQC) schemes, are of utmost importance to maintain the good predictive value of sperm morphology.

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“…An abnormally elongated nucleus shape of these spermatozoa is related with defects in the nuclear membrane, an increased frequency of chromosomal aneuploidies and altered chromatin compaction. Also anomalies of the neck region, persistence of cytoplasmic residues can often exist in these spermatozoa [52,53].…”

Section: Abnormalities Of the Headmentioning

confidence: 99%

Assessment of Human Sperm Cells Morphological Parameters

Lasienë¹

2018

Spermatozoa - Facts and Perspectives

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The quality of spermatozoa has a direct influence on the fertilization and developmental competence of embryos. The aim of this work was to review the methods of spermatozoa morphology assessment, features of the normal spermatozoa and the reasons of their several abnormalities. Three methods can be used for the evaluation of spermatozoa morphology in the in vitro fertilization (IVF) laboratory: (1) light microscopy of stained spermatozoa, (2) motile sperm organelle morphology examination (MSOME) and (3) polarized light microscopy. The analysis of spermatozoa morphology includes the assessment of head, neck, midpiece and tail. Morphologically abnormal spermatozoa are categorized into subgroups according to the defects of the head, neck, midpiece and/or tail. Before IVF and intracytoplasmic sperm injection (ICSI), the quality of spermatozoa must be estimated exactly, because this has the high influence on embryo development. Therefore the analysis of the morphological parameters of spermatozoa using the light microscopy, MSOME, in combination with precise head birefringence detection using the polarized microscopy, could give the best fertilization rate and embryo quality after IVF and ICSI.

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Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads

Abstract: This study demonstrates that impaired chromatin compaction and slightly increased chromosome aneuploidies are found in spermatozoa with an elongated head, suggesting possible mechanisms such as meiotic non-disjunctions or spermiogenesis anomalies.

Cited by 36 publications

References 45 publications

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen

Assessment of Human Sperm Cells Morphological Parameters

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