Ultrastructural Modifications of the Mouse Uterus upon Biogenic Amine Stimulation

Obayomi, S. M. Bukola; Peck, S.; Baluch, D. Page

doi:10.1017/s1431927619006512

Cited by 2 publications

(2 citation statements)

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“…Tyramine was shown to colocalize with synaptic vesicles in the mouse uterine plexus, highlighting a possible role in uterine contractions 48,49 . Altogether, these results highlight the potential connection between vaginal metabolites, metabolite levels in various reproductive organs, and preterm delivery.…”

Section: Metabolite Associations With Sptb Interact With Race and Sptb Timingmentioning

confidence: 99%

Preterm birth is associated with xenobiotics and predicted by the vaginal metabolome

Kindschuh

Baldini

Liu

et al. 2021

Preprint

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Spontaneous preterm birth (sPTB) is a leading cause of maternal and neonatal morbidity and mortality, yet both its prevention and early risk stratification are limited. The vaginal microbiome has been associated with PTB risk, possibly via metabolic or other interactions with its host. Here, we performed untargeted metabolomics on 232 vaginal samples, in which we have previously profiled the microbiota using 16S rRNA gene sequencing. Samples were collected at 20-24 weeks of gestation from women with singleton pregnancies, of which 80 delivered spontaneously before 37 weeks of gestation. We find that the vaginal metabolome correlates with the microbiome and separates into six clusters, three of which are associated with spontaneous preterm birth (sPTB) in Black women. Furthermore, while we identify five metabolites that associate with sPTB, another five associate with sPTB only when stratifying by race. We identify multiple microbial correlations with metabolites associated with sPTB, including intriguing correlations between vaginal bacteria that are considered sub-optimal and metabolites that were enriched in women who delivered at term. We propose that several sPTB-associated metabolites may be exogenous, and investigate another using metabolic models. Notably, we use machine learning models to predict sPTB risk using metabolite levels, weeks to months in advance, with high accuracy. We show that these predictions are more accurate than microbiome-based and maternal covariates-based models. Altogether, our results demonstrate the potential of vaginal metabolites as early biomarkers of sPTB and highlight exogenous exposures as potential risk factors for prematurity.

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Section: Metabolite Associations With Sptb Interact With Race and Sptb Timingmentioning

confidence: 99%

Preterm birth is associated with xenobiotics and predicted by the vaginal metabolome

Kindschuh

Baldini

Liu

et al. 2021

Preprint

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show abstract

“…Dopamine is a neurotransmitter that has been shown to inhibit smooth muscle contractions while a recent study has found that tyramine, a biogenic amine from the same biosynthetic pathway, and its specific receptor TAAR1 (trace amine associated receptor 1), are localized within the region of the uterine plexus and can induce uterine contractions in vitro. It was also observed that there was an increased synaptic vesicle recruitment in tyramine treated uterine tissues [2]. Research in multiple mammals, including humans, has shown pinocytic vesicle formation along the lumen of the uterus which is thought to serve as uptake mechanism to reduce inner lumen volume to draw the embryo close for implantation [3].…”

mentioning

confidence: 98%

Tyramine Localization Closely Corelates to Circular Vesicles Within the Mouse Uterine Horn Using Correlational Fluorescence and Scanning Electron Microscopy

Obayomi

Baluch²

2020

Microsc Microanal

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Ultrastructural Modifications of the Mouse Uterus upon Biogenic Amine Stimulation

Cited by 2 publications

References 4 publications

Preterm birth is associated with xenobiotics and predicted by the vaginal metabolome

Preterm birth is associated with xenobiotics and predicted by the vaginal metabolome

Tyramine Localization Closely Corelates to Circular Vesicles Within the Mouse Uterine Horn Using Correlational Fluorescence and Scanning Electron Microscopy

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