Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)

Abstract

Calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate-pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.

Subcellular distribution of calcium during spermatogenesis of zebrafish, Danio rerio

“…While most calcium is deposited in limited areas during the spermatogonium and spermatocyte stages, it is mostly detected as unbound pools (free calcium) which were released from dense isolated deposits of earlier stages of spermatogenesis and distributed in larger areas of the spermatid and the spermatozoon particularly nucleus. Similar changes in the ultrastructural distribution of calcium were observed during spermatogenesis of sterlet, Acipencer ruthenus (Golpour et al, ).…”

Retracted: Gonad histology and serum 11‐KT profile during the annual reproductive cycle in sterlet sturgeon adult males, Acipenser ruthenus

“…Only preliminary results dealing with gonadal development in cultured sterlet have been published previously (Jackson et al., ). Moreover, study on annual gonadal cycle and changes in serum sex steroid profile of fish is crucial to examine germline development and spawning pattern in fish (Barcellos et al., ; Golpour, Pšenička, & Niksirat, ; Lee & Yang, ; Manosroi, Meng‐Umphan, & Manosroi, ; Tan‐Fermin, Ijiri, Ueda, Adachi, & Yamauchi, ). Gametogenesis and gonadal cycles were evaluated in cultured male and female of white sturgeon, Acipenser transmontanus (Doroshov, Moberg, & Van Eenennaam, ), female siberian sturgeon, Acipenser baerii (Williot, Brun, Rouault, & Rooryck, ), male and female of amur sturgeon, Acipenser schrenckii (Qu, Sun, Wan, & Ma, ), and both sexes of shortnose sturgeon, Acipenser brevirostrum (Colombo, Garvey, & Wills, ; Flynn & Benfey, ).…”

“…Moreover, study on annual gonadal cycle and changes in serum sex steroidprofileoffishiscrucialtoexaminegermlinedevelopmentand spawning pattern in fish (Barcellos etal., 2001;Golpour, Pšenička, & Niksirat, 2016b;Lee & Yang, 2002;Manosroi, Meng-Umphan, & Manosroi, 2003;Tan-Fermin, Ijiri, Ueda, Adachi, &Yamauchi, 1997).…”

Seasonal Changes in the Milt Quality of Waigieu Seaperch, Psammoperca waigiensis: Implications for Artificial Propagation

“…The head of sperm (<2 μm in width) in Waigieu seaperch was bigger than that of yellow croaker (<1.6 μm in width) (Le et al ) and filefish (<1.4 μm in width) (Le et al ), but smaller than that in other teleost fishes (from 2 to 4 μm in width) (Jamieson ). Additionally, the sperm has the kidney shape that is similar to Mediterranean rainbow wrasse, Coris julis (Jamieson ), and yellow croaker (Le et al ), but is different from other teleost fish such as ball‐shaped in northern pike, Esox lucius; ovoid‐shaped in cardinal fish, Apgon imberbis ; banana‐shaped in Atlantic eel, Anguilla anguilla (Jamieson ); horse‐shoe‐shaped in filefish (Le et al ); and elongated in sturgeon (Golpour et al ). Alavi et al () reported the changes in sperm morphological and ultrastructural parameters in the freshwater species Barbus barbus during the spawning season.…”