Ultrastructural localization of ependymins in the endomeninx of the brain of the rainbow trout: possible association with collagen fibrils of the extracellular matrix

Schwarz, Heinz; Müller-Schmid, Angelika; Hoffmann, Werner

doi:10.1007/bf00333696

Cited by 27 publications

(19 citation statements)

References 35 publications

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“…Subsequently, the sections were treated with anti-hTFF3-1 antiserum (dilution 1:500), which was detected by rabbit anti-chicken 12-nm gold complexes (#303-205-033; Jackson Immuno Research, West Grove, Pa., USA). The labelled sections were stained with uranyl acetate and lead citrate for electron microscopy as previously described (Schwarz et al 1993).…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Ultrastructural co-localization of TFF3-peptide and oxytocin in the neural lobe of the porcine pituitary

Schwarz

Jagla

Wiede

et al. 2001

Cell and Tissue Research

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TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of many mucous epithelial cells. TFF3 is also synthesized in oxytocinergic neurons in the paraventricular and supraoptic nuclei of the human hypothalamus. Here, TFF3 and oxytocin are shown to be co-localized within the same secretory vesicles in the neural (posterior) lobe of the procine pituitary by means of immunoelectron microscopy. Relatively large amounts of TFF3, but not TFF1 and TFF2, are present in the neural lobe of the porcine pituitary, where it is probably released into the bloodstream.

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Section: Immunoelectron Microscopymentioning

confidence: 99%

Ultrastructural co-localization of TFF3-peptide and oxytocin in the neural lobe of the porcine pituitary

Schwarz

Jagla

Wiede

et al. 2001

Cell and Tissue Research

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“…Thin sections were either mounted on nickel grids or polylysine-coated coverslips. Immunostaining of the sections was performed according to Schwarz et al (1993). As a first antibody a rabbit anti biotin antibody (Ortho Diagnostics, Raritan, NJ) was used at a concentration of 5 Fg/ml.…”

Section: Electron Microscopymentioning

confidence: 99%

Epithelial cell polarity in early Xenopus development

Müller

Hausen

1995

Developmental Dynamics

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The Xenopus blastula consists of two morphologically distinct cell types. Polarized epithelial cells build up the embryonic surface and fence off an inner non-polarized cell population. We examined the establishment of this early functional cell diversification in the embryo by single cell analysis, in vitro cell culture, and transplantation experiments. Single blastomeres from a 64-cell embryo (1/64 cells) exhibit several features of polarized cells. The plasma membrane of 1/64 cells consists of an apical domain, which is inherited from the original egg membrane, and a basolateral domain derived from newly formed membrane during cleavage. These are inherent, cell-autonomous properties of the blastomeres, as they form and are maintained in blastomeres raised in the absence of any cell interactions in calcium free medium. Upon in vitro culture a single 1/64 cell gives rise to an aggregate of two different cell types. Cells carrying a part of the former egg membrane domain differentiate into polarized epithelial cells, whereas cells lacking this membrane domain are not polarized. These results demonstrate that the inclusion of the egg membrane, rather than external signals related to the position of a cell in the intact embryo, is required for the apicalhasolateral differentiation of the surface epithelium. This view is supported by cell transplantation studies. A single 1/64 cell was implanted into the blastocoel of a stage 8 blastula embryo. The progeny of the implanted cell proliferate within the host embryo and split into two morphologically distinct populations with different cell behaviours. Cells incorporating a part of the egg membrane form coherent patches of polarized epithelial cell sheets in the interior of the host embryo. In contrast, cells lacking egg membrane do not exhibit any characteristics of polarized cells and eventually spread into different regions of the host embryo. Our results show that the egg membrane and/or components of the submembrane cortex play a determinative role in the formation of the blastula epithelium.

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“…Hildebrand 1995) as being single-layered (the 'meninx primitiva' of Sterzi 1901), but the few detailed light and electron microscopical studies that have been done on this structure indicate that it has a more complex organization (Rovainen et al 1971;Nakao 1979;Momose et al 1988;Caruncho et al 1993;Schwarz et al 1993;Wang et al 1995). Only one of these reports (Nakao 1979), however, has focused on the spinal cord (of lamprey), so we first describe the organization of the meningeal sheath of the uninjured spinal cord of the eel, before assessing its structure and significance during regeneration.…”

Section: Introductionmentioning

confidence: 97%

The meningeal sheath of the regenerating spinal cord of the eel, Anguilla

Dervan

Roberts

2003

Anatomy and Embryology

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We describe here the meningeal sheath that encloses the spinal cord, and the sheath that develops when the cord regenerates after a total transection. This description is derived from electron and light microscopy. The sheath of the uninjured cord was found to be a single structure of two parts: an outer, thin melanocyte layer and an inner, thicker layer of 2 to 10 rows of fibroblasts, closely associated with collagen and elastic fibers. Soon after cord transection, the injured axons re-grow and, together with the reforming central canal, create a bridge that links the transected cord within 8 days of injury. This bridge is covered at first by a rudimentary meningeal sheath, formed of fibroblasts and macrophages, that later progressively thickens and becomes more compact. By about day 20, the fibroblasts are arranged as 16 to 20 loose rows that include bundles of collagen, oriented along the rostro-caudal axis of the cord. Even after 144 days, the meninx, although substantially thicker than normal because of the numerous fibroblast rows (20 to 30), still lacks the melanocyte layer. In cases in which the meninx at the transection site was mechanically and pharmacologically (6-hydroxydopamine) disrupted, bridge formation was essentially unchanged, and axonal regrowth continued; some regrowing axons, however, extruded from the denuded cord. Accordingly, our findings indicate that although the meningeal sheath is not essential for cord regeneration to take place, it may well facilitate recovery by providing mechanical guidance and support to the regrowing axons.

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Ultrastructural localization of ependymins in the endomeninx of the brain of the rainbow trout: possible association with collagen fibrils of the extracellular matrix

Cited by 27 publications

References 35 publications

Ultrastructural co-localization of TFF3-peptide and oxytocin in the neural lobe of the porcine pituitary

Ultrastructural co-localization of TFF3-peptide and oxytocin in the neural lobe of the porcine pituitary

Epithelial cell polarity in early Xenopus development

The meningeal sheath of the regenerating spinal cord of the eel, Anguilla

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