Ultrastructural localization of endogenous hormones and receptors in bone tissue: An immunocytological approach in frozen samples

Boivin, Georges; Anthoine-Terrier, Chantal; Morel, Gérard

doi:10.1016/0968-4328(94)90052-3

Cited by 14 publications

(7 citation statements)

References 84 publications

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“…To date, there have been no reports of the presence of PR mRNA or protein (7,15) in human adipose tissue, although specific progestin binding has been demonstrated in the adipose tissue of rat (16) and sheep (17). In addition to traditional target tissues, PR has been identified in such nonclassical human tissues as osteoclasts (18), the peripheral veins (19), and prostate stromal cells (20).…”

Section: Discussionmentioning

confidence: 95%

Identification of Progesterone Receptor in Human Subcutaneous Adipose Tissue

O’Brien

Welter

Mantzke

et al. 1998

The Journal of Clinical Endocrinology & Metabolism

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Sex steroids are postulated to play a role in adipose tissue regulation and distribution, because the amount and location of adipose tissue changes during puberty and menopause. Because of the nature of adipose tissue, receptors for the female sex steroids have been difficult to demonstrate. To date, estrogen receptor messenger RNA and protein have been identified in human subcutaneous adipose tissue, but the presence of progesterone receptor (PR) has not been reported. In this study, we demonstrate PR message by Northern blot analysis in RNA isolated from the abdominal subcutaneous adipose tissue of premenopausal women. These preliminary studies revealed that PR messenger RNA levels are higher in the stromal-vascular fraction as opposed to the adipocyte fraction. Western blot analysis demonstrates both PR protein isoforms (human PR-A and human PR-B) in human subcutaneous adipose tissue. Using an enzyme-linked immunosorbent assay, total PR could be quantitated. These studies substantiate that sex steroid receptors are present in human adipose tissue, thereby providing a direct route for regulation of adipose tissue by female sex steroids.

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Section: Discussionmentioning

confidence: 95%

Identification of Progesterone Receptor in Human Subcutaneous Adipose Tissue

O’Brien

Welter

Mantzke

et al. 1998

The Journal of Clinical Endocrinology & Metabolism

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“…Tissues which contain PR include the breast (Yu et al, 1994a), the endometrium (Clements and Mukhtar, 1994), brain meningiomas (Carrol et al, 1993), blood vessel walls (Bergqvist et al, 1993), urinary tract (Pacchioni et al, 1992) and osteoclasts (Boivin et al, 1994). In view of these and other findings, we believe that it is time to study in detail the biological role of PSA in non-prostatic tissues.…”

Section: Discussionmentioning

confidence: 96%

Steroid hormone regulation of prostate-specific antigen gene expression in breast cancer

Zarghami

Grass²,

Diamandis³

1997

Br J Cancer

113

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Summary We have recently reported that about 30-40% of female breast tumours produce prostate-specific antigen (PSA) and that PSA production is associated with the presence of oestrogen (ER) and progesterone (PR) receptors. We have now developed a tissue culture system to study the regulation of the PSA gene in breast cancer. The breast carcinoma cell line T-47D produces PSA when stimulated by androgens, progestins and glucocorticoids/mineralocorticoids but not oestrogens. PSA mRNA appears approximately 2 h after stimulation; PSA protein appears after 4-8 h. Among 38 compounds tested, only androgens and progestins were able to stimulate PSA production at concentrations below 10-9 M. Evidence that the progesterone and androgen receptors can regulate the PSA gene independently was provided as follows: (a) the progestin norgestimate, which does not bind to the androgen receptor, up-regulates the PSA gene at concentrations as low as 10-10 M; (b) triamicinolone acetonide, which does not bind to the androgen receptor (AR) but binds to the PR, acts similarly to norgestimate; (c) the antiandrogen cyproterone acetate, which blocks the androgen receptor but has progestational activity, up-regulates the PSA gene at concentrations as low as 10-10 M; (d) the antiprogestin mifepristone completely blocks the stimulation of the specific progestin norgestimate. Our tissue culture system identified androgen -progestin agonist activities of 17a-ethinyloestradiol, the antioestrogen RU56, 187 and the antiprogestin mifepristone. Our data suggest that the expression of the PSA gene in the female breast is under the control of androgens and progestins. Our tissue culture system is a highly sensitive in vitro method for evaluating the biological activity of candidate compounds having agonist and antagonist steroid hormone activity.

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“…PR mRNA and protein have also been detected in human osteoblastic cells [185,[210][211][212], and MacNamara and Loughrey [213] have recently shown that transcripts encoding the A and B forms of the PR (two isoforms transcribed from distinct promoters within the same gene [214]) are present. Immunohistochemical analysis of human bone localized PR protein to both osteoblasts [215] …”

Section: Progesterone Receptor (Pr) Expressionmentioning

confidence: 99%

Steroid hormone receptor expression and action in bone

BLAND

2000

Clinical Science

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The skeleton is a complex tissue, and hormonal control of bone remodelling is elaborate. The important role that steroid hormones play in bone cell development and in the maintenance of normal bone architecture is well established, but it is only relatively recently that it has become possible to describe their precise mechanism of action. This review focuses not only on the steroid hormones (oestrogens, corticosteroids, androgens and progesterone), but also on related hormones (vitamin D, thyroid hormone and the retinoids), all of which act via structurally homologous nuclear receptors that form part of the steroid/thyroid receptor superfamily. By examining the actions of all of these hormones in vivo and in vitro, this review gives a general overview of the current understanding of steroid hormone action in bone. In addition, a comprehensive review of steroid hormone receptor expression in bone cells is included. Finally, the role that future developments, such as steroid hormone receptor knockout mice, will play in our understanding of steroid hormone action in bone is considered.

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Ultrastructural localization of endogenous hormones and receptors in bone tissue: An immunocytological approach in frozen samples

Cited by 14 publications

References 84 publications

Identification of Progesterone Receptor in Human Subcutaneous Adipose Tissue

Identification of Progesterone Receptor in Human Subcutaneous Adipose Tissue

Steroid hormone regulation of prostate-specific antigen gene expression in breast cancer

Steroid hormone receptor expression and action in bone

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