Ultrastructural localization of calcium in the pigeon papillary muscle as demonstrated by cytochemical studies and X-ray microanalysis

Sætersdal, Thorvald; Myklebust, R.; Justesen, N.‐P. Berg; Olsen, W. Cato

doi:10.1007/bf00220284

Cited by 25 publications

(6 citation statements)

References 27 publications

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“…x 54,000 . distribution of Ca l l in the sarcoplasmic reticulum of pigeon ventricular muscle by pyroantimonate precipitation (29), which suggested that both the peripheral junctional and the corbular sarcoplasmic reticulum in pigeon myocardial cells might be involved in the regulation of cytoplasmic Ca 2+ during the contraction-relaxation cycle. However, the localization of Ca l l by this technique might be misleading due to diffusion of Caz+ during the precipitation procedure.…”

Section: Discussionmentioning

confidence: 96%

“…Since calsequestrin localized in the interior regions of the cytoplasm is mostly confined to the lumen of vesicular structures in the interfibrillar spaces in the I-band region but absent from vesicular structures in the A-band region, it is likely that the calsequestrin-containing vesicles present in the I-band region correspond to the specialized extensions on the free sarcoplasmic reticulum called corbular sarcoplasmic reticulum (27). distribution of Ca l l in the sarcoplasmic reticulum of pigeon ventricular muscle by pyroantimonate precipitation (29), which suggested that both the peripheral junctional and the corbular sarcoplasmic reticulum in pigeon myocardial cells might be involved in the regulation of cytoplasmic Ca 2+ during the contraction-relaxation cycle. However, the localization of Ca l l by this technique might be misleading due to diffusion of Caz+ during the precipitation procedure.…”

Section: Discussionmentioning

confidence: 99%

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Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum.

Jorgensen¹,

Campbell²

1984

The Journal of Cell Biology

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Localization of calsequestrin in chicken ventricular muscle cells was determined by indirect immunofluorescence and immuno-Protein A-colloidal gold labeling of cryostat and ultracryotomy sections, respectively . Calsequestrin was localized in the lumen of peripheral junctional sarcoplasmic reticulum, as well as in the lumen of membrane-bound structures present in the central region of the I-band, while being absent from the lumen of the sarcoplasmic reticulum in the A-band region of the cardiac muscle cells . Since chicken ventricular muscle cells lack transverse tubules, the presence of calsequestrin in membrane bound structures in the central region of the 1-band suggests that these cells contain nonjunctional regions of sarcoplasmic reticulum that are involved in Ca" storage and possibly Ca" release . It is likely that the calsequestrin containing structures present throughout the 1-band region of the muscle cells correspond to specialized regions of the free sarcoplasmic reticulum in the 1-band called corbular sarcoplasmic reticulum . It will be of interest to determine whether Ca" storage and possibly Ca" release from junctional and nonjunctional regions of the sarcoplasmic reticulum in chicken ventricular muscle cells are regulated by the same or different physiological signals.It is generally agreed that the sarcoplasmic reticulum plays an important role in the regulation of the cytoplasmic Cal' concentration and thereby the state ofcontraction and relaxation in mammalian and avian cardiac muscle (1-6). Determination of the subcellular distribution of calcium by autoradiography (7) and electron microprobe x-ray analysis (8,9) have clearly demonstrated that the lumen of the sarcoplasmic reticulum stores Ca l ' during relaxation and then releases Call following the depolarization of the transverse tubular membrane to induce contraction in frog skeletal muscle . Biochemical studies (10, 11) and immunocytochemical studies (12-14) have suggested that most of the sarcoplasmic reticulum protein calsequestrin is localized in the lumen of the terminal cisternae in rabbit (10,11,14) and rat (12, 13) skeletal muscle. These results strongly support the view that the function of the calcium binding protein calsequestrin in the lumen of the junctional sarcoplasmic reticulum is to "sequester" calcium during the relaxation of skeletal muscle (15, 16) .Recently we have identified, purified, and characterized calsequestrin from canine ventricular muscle tissue (17). In the same study, indirect immunofluorescence labeling showed that most of the calsequestrin is localized in the I-band region of rat and canine ventricular muscle cells, suggesting that most of the cardiac calsequestrin, like skeletal calsequestrin, THE JOURNAL OF CELL BIOLOGY -VOLUME 98 APRIL 1984 1597-1602 0 The Rockefeller University Press -0021-9525/84/04/1597/06 $1 .00 is confined to the region of the sarcoplasmic reticulum that is in close apposition to either the sarcolemma or the transverse tubules (i.e., the lumen of the peripheral and inter...

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum.

Jorgensen¹,

Campbell²

1984

The Journal of Cell Biology

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“…Several other groups have reported that intracellular antimonate deposits that have been shown by x-ray analysis to contain Ca" are not visible after either EGTA or EDTA treatment (7,18,47,48); Wooding and Morgan (49) found similar results with EDTA. Embedded K+-antimonate is not solubilized by distilled water or by chelators, and in vitro Na'-antimonate is likewise unaffected by EDTA or EGTA (37,47). Were K+ or FIGURE 8 Metaphase.…”

Section: Antimonate Localization Through Mitosis In Hordeurn Vulgarementioning

confidence: 99%

Localization of Ca++-containing antimonate precipitates during mitosis.

Wick¹,

Hepler²

1980

The Journal of Cell Biology

125

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Intracellular bound Ca" has been localized throughout mitosis and cytokinesis in two plant species by means of in situ precipitation with potassium antimonate and electron microscope visualization . Identification of Ca" as the major cation precipitated was made by comparing solubility properties in water, EDTA, and ECTA of the intracellular deposits with respect to those of K +-, Mg"-, and Ca"-antimonate standards. In spermatogenous cells of the water fern, Marsilea vestita, and stomatal complex cells of barley, Hordeum vulgare, antimonate deposits have been found associated with the endoplasmic reticulum (ER), vacuoles, euchromatin/nucleoplasm, and mitochondria . The last contain a much higher density of precipitates in Marsilea than in Hordeum . Dictyosomes and the nuclear envelope of Marsilea also contain antimonate deposits, as do the plasmalemma, cell wall, and phragmoplast vesicles of Hordeum . Microtubule-organizing centers such as kinetochores and the blepharoplast of Marsilea do not stain . In spite of differences in associated antimonate between certain organelles of the two species, the presence of antimonate along the ER throughout the cell cycle is common to both .Of particular interest are those precipitates seen along the tubules and cisternae of the extensive smooth ER that surrounds and invades the mitotic spindle in both species. The ability to bind divalent cations makes the mitotic apparatus (MA)-associated ER a likely candidate for regulation of free Ca" levels in the immediate vicinity of structural components and processes that are Ca'-sensitive and proposed to be Ca"-regulated.

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“…The precipitation of pyroantimonate with calcium has been con¬ firmed by (1) X-ray microanalysis (e.g. Saetersdal, Myklebust, Justesen & Olsen, 1974;Chandler & Battersby, 1976; Weakley, 1979;O'Brien & Baumrucker, 1980;Salisbury, 1982), (2) use of the calcium-chelating agent, ethyleneglycol te(aminoethylether)tetra-acetic acid (EGTA), before (Legato & Langer, 1969;Klein, Horton & Thureson-Klein, 1970;Klein, Yen & Thureson-Klein, 1972; Weakley, 1979) or after (Weakley, 1979;Kashiwa & Thiersch, 1984) fixation, or (3) floating sectioned material on an EGTA solution (Salisbury, 1982). Wick & Hepler (1982) …”

Section: Introductionmentioning

confidence: 99%

Ultrastructural localization of calcium ions in ram spermatozoa before and after cold shock as demonstrated by a pyroantimonate technique

Plummer¹,

Watson²

1985

Reproduction

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Summary. Ram spermatozoa were subjected to cold shock before fixation in pyroantimonate\p=n-\osmium. Ultrathin sections revealed an electron-dense particulate precipitate in association with the cells. The precipitate was shown to be related to the presence of calcium by exposure of the material to EGTA which reduced or completely eliminated the deposits.In the acrosome region, very little precipitate was evident when the plasma membrane was intact. Cold shock resulted in the disruption of the plasma membrane. When the acrosome remained intact, precipitate was concentrated just anterior to the equatorial segment, but many cells also had acrosomal disruption and then a more even distribution of precipitate was seen on the outer acrosomal membrane. Precipitate was rarely visible within or beneath the acrosome. Post-acrosomally, calcium pyroantimonate deposits were frequently present in the dense lamina beneath the plasma membrane and these became more intense after cold shock.Midpiece sections revealed a few large granules beneath the plasma membrane and a fine particulate precipitate within mitochondria. Similarly, the fine precipitate was also associated with the outer dense fibres in midpieces and tails. Cold shock did not apparently increase the extent or intensity of precipitates in these sites.

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Ultrastructural localization of calcium in the pigeon papillary muscle as demonstrated by cytochemical studies and X-ray microanalysis

Cited by 25 publications

References 27 publications

Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum.

Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum.

Localization of Ca++-containing antimonate precipitates during mitosis.

Ultrastructural localization of calcium ions in ram spermatozoa before and after cold shock as demonstrated by a pyroantimonate technique

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