Ultrastructural Immunohistochemistry of Glomerulonephritisin Needle Biopsy

Rossmann, P; Jirka, J; Matoušovic, K; Zastava, V; Šefcová, J.

doi:10.1016/s0344-0338(84)80107-7

Cited by 11 publications

(2 citation statements)

References 28 publications

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“…Owing to the low morphological resolution of IF, it is difficult to determine the precise localization of the immune complex deposits inside the glomerulus. It is possible that the positive fluorescence, most often for IgG, was caused by labeling antigens in the plasma in the capillary lumen or by labeling antigens which had leaked I47 from the capillaries into the glomerular tissue (13). This theory is supported by the fact that IgG is the immunoglobulin with the highest concentration in plasma, and corresponds well with previous studies (14).…”

Section: Iem As Diagnostic Toolsupporting

confidence: 85%

Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases

Brorson

Strøm

Skjørten

1997

APMIS

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Twenty renal biopsies were studied by immunoelectron microscopy (IEM) after embedding in epoxy resin. Immunogold labeling for immunoglobulins and complement C3 was performed on the epoxy sections, which were not subjected to any kind of etching or deplasticizing prior to the immunolabeling. The concentration of accelerator, DMP‐30 (Tri (Dimethyl Amino Methyl) Phenol), was increased in the infiltration and embedding steps far beyond the values normally used to make immunolabeling of these antigens possible on epoxy sections. The sections were stained with tannic acid accompanied by uranyl acetate and lead citrate. Immunofluorescence (IF) for light microscopy was carried out on frozen sections of parallel tissue samples. Some cases with IgA‐nephritis demonstrated a higher sensitivity for IEM than IF, in the sense that smaller amounts of antigen were detectable with IEM. Ultrastructural preservation with this method was approximately the same as that usually seen on epoxy‐embedded material. By combining excellent immunolabeling with nearly optimal ultrastructural morphology in one procedure, this method is useful particularly in situations where the material available is limited, such as in studies of renal biopsies. As far as we know, this is the first time that immunoglobulins have been satisfactorily immunolabeled on epoxy sections without etching or deplasticizing.

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Section: Iem As Diagnostic Toolsupporting

confidence: 85%

Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases

Brorson

Strøm

Skjørten

1997

APMIS

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“…This might be carried out on frozen sections obtained from glomeruli concentrated in tissue embedding medium, such as OCT. Furthermore, elegant, immunolabeling investigations might more easily be carried out on isolated glomeruli, where penetration of reagents has been shown to be of value (Rossmann et al, 1984; Schreiner and Unanue, 1984). These avenues of investigation may prove important in serial investigation of the pathogenesis of kidney diseases where precise knowledge of anatomical localization of antigens is important.…”

Section: Discussionmentioning

confidence: 99%

Transmission electron microscopic analysis of kidney glomeruli obtained by fine‐needle aspiration

Rowden

Dean

Campbell

et al. 1985

J. Elec. Microsc. Tech.

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Aspiration biopsy of tumors and transplanted organs is a popular atraumatic method for diagnosis and also for the management of patients. Apart from the cytological analyses, it is clear that large aggregates of cells are obtained and these may be examined by electron microscopy. A study was carried out with dogs to assess the yield of intact glomeruli and tubules, resulting from a standard fine-needle aspiration procedure. Employing a 20-gauge needle, yields of approximately ten intact glomeruli were obtained with a tissue processing protocol that utilized filtration through a nylon mesh and backwashing, to resuspend the trapped aggregates. Processing for electron microscopy was carried out in microcentrifuge tubes. The ultrastructural preservation was excellent and the extension of these studies to investigations of not only renal transplantation, but also for diagnosis in human renal diseases of a broad spectrum, is suggested.

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Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis

et al. 1986

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The ultrastructural localization of immunoglobulins (Igs) in immunologically mediated glomerulonephritis was achieved in ultrathin sections of glutaraldehyde-fixed, post-osmicated, Epon-embedded human renal needle biopsies using the indirect immunogold staining procedure. Extracellular Igs were identified in electron-dense deposits in subendothelial, intramembranous, subepithelial and mesangial locations in different cases of immune-complex-mediated glomerulonephritis. The excellent preservation of the ultrastructural morphology of the tissue sections made it possible to precisely correlate the presence and distribution of the antigens with those of the electron-dense deposits, which are the ultrastructural hallmarks of these diseases. Our post-embedding staining procedure which allows extracellular Igs to the accurately localized should be a very useful tool for immunomorphological investigations of glomerulonephritis.

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Ultrastructural Immunohistochemistry of Glomerulonephritisin Needle Biopsy

Cited by 11 publications

References 28 publications

Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases

Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases

Transmission electron microscopic analysis of kidney glomeruli obtained by fine‐needle aspiration

Ultrastructural localization of extracellular immunoglobulins in immune-complex-mediated glomerulonephritis

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